specific biotechnological goal of this area of research is the
development of biocatalytic devices for analytical sensing. Such
analytical, bioelectronic devices may lead to enzyme sensors, made
independent of molecular oxygen or co-factors often needed for their
function. Of particular interest is the development of fast,
highly-selective and regenerable amperometric biosensors that are based
on the flow of direct interfacial current between metallic or
semiconductor electrodes and immobilized enzymes.
although longer term technological goal, is the development of in-vivo
devices that may be directly involved in electrical communication with
signal producing or controlling molecules, organelles or structures
within living systems.
projects include: (i) direct electrochemistry and characterization of
cytochrome C Oxidase in bilayer modified-metallic electrodes. (ii) The
development of biocompatible electrodes using ultrathin self-assembled
organic films. (iii) Nanoparticle modification of glassy carbon and
microfabricated electrodes for biological redox mediation.
in bioelectronics and bioelectrochemistry: (i) Techniques and
procedures for reproducibly preparing bioactive monolayers on solid
state electrodes. (ii) Retained bioactivity in bioelectronic layer.
(iii) Vulnerability of the biotransducer to foulants and interferences.
directions in bioelectronics and bioelectrochemistry: (i) The
use of genetically engineered proteins for control of biophysical
properties and enhanced stability in mono and bi-layers. (ii) Methods
for electrode surface modification for enhanced stability and of
bioactive layers. (iii) The use of enzyme modified electrodes to assay
pharmaceutical enzyme inhibitors. (iv) The use of cytochrome c oxidase
modified electrodes to assay toxins. (v) The development of
high-throughput methods using enzyme modified electrodes.
in molecular bioelectronics and bioelectrochemistry have the potential
to greatly impact our future approaches to in-vivo and invitro analysis
and to therapy.