C3B InVivo Biosensors Challenges

Biocompatibility Issues and approaches Use of multifunctional, heterogeneous, biomimetic materials Emulate the extracellular matrix – form and function Employ transplant strategies – site specific and systemic Controlled release of mediators – local modulation Sensor performance Interferences – protein adsorption and denaturation (fouling) Materials reliability Telemetry Data at a distance Informatics Complexity – space and time Multiple data sources – plurality of inputs Attachment of the bioactive layer to the solid state transducer Stability of the bioactive layer in the biotransducer Large scale manufacture - (The I-Stat story) Biocompatibility of in vivo biosensors Reproducible placement of small volumes of bioactive materials onto devices Microarraying and microfluidic techniques. Photolithographic patterning of bioactive layers