Ph.D., University of Windsor; 1993; Biology
B.Sc., McGill University; 1987 Microbiology and Immunology
The overall goal of the research in Dr. Temesvari's laboratory is to understand the pathogenesis of the human protozoan parasite, Entamoeba histolytica. This is the causative agent of amoebic dysentery and amoebic liver abscess; up to 50,000,000 people world-wide are infected annually. Secretion, phagocytosis, and parasite-host interactions are important virulence functions of E. histolytica. Thus, using state-of-art molecular, biochemical, genetic and cellular techniques, the Temesvari laboratory seeks to understand the virulence functions by studying (i) four Rab GTPases (master regulators of vesicle trafficking), (ii) several components of the PI 3-kinase signaling pathway, and (iii) lipid rafts. Insight gained through these studies may lead to rational design of novel anti-Entamoeba agents.
Koushik, A.B., Powell, R.R., and Temesvari, L.A. Localization of phosphatidylinositol 4,5-bisphosphate to lipid rafts and uroids in the human protozoan parasite, Entamoeba histolytica. Infect Immun. 81, 2145-2155.
King, A.V., Welter, B.H., Koushik, A.B., and Temesvari, L.A. A Genome-Wide Over-Expression Screen Identifies Genes Involved in Phagocytosis in the Human Protozoan Parasite, Entamoeba histolytica, PloS ONE 7, e43025.
Goldston A.M., Powell R.R., Koushik A.B., and Temesvari L.A. Exposure to Host Ligands Correlates with Co-localization of Gal/GalNAc Lectin Subunits in Lipid Rafts and PIP2 Signaling in Entamoeba histolytica. Eukaryot. Cell. 11, 743-751. Editors’ Choice Feature Article
Goldston, A. M., Powell, R.R., and Temesvari, L.A. Sink or swim: lipid rafts in parasite pathogenesis. Trends Parasitol. 28, 417-426.
Welter, B.H., Goldston, A.M., and Temesvari, L.A. Localisation to lipid rafts correlates with increased function of the Gal/GalNAc lectin in the human protozoan parasite, Entamoeba histolytica. Int. J. Parasitol. 41, 1409-1419.