Professor, Biological Sciences
Professor, Environmental Toxicology
Biological Sciences Department
Office: 239 Long Hall
Fax: (864) 656-0435
North Carolina State University 1997
B.S. Environmental Health Sciences
University of Georgia 1992
ETOX 6300 - Toxicology
BIOL 4610 - Cell Biology
BIOL 4930 - Senior Seminar
Understanding the mechanisms by which arsenic (and other chemicals) impact development and cell fate determination
Research in the Bain laboratory focuses on the mechanisms by which cells respond to toxicants, such as arsenic, chromium, and pharmaceuticals. We are currently investigating how arsenic delays cellular and organismal differentiation and development. Arsenic is a contaminant in drinking water in many parts of the world, and has been found at appreciable levels in rice and other crops. Arsenic readily crosses the placental barrier and exposure is correlated with adverse developmental outcomes such as stillbirths, spontaneous abortions, neonatal mortality, low birth weight, delays in the use of musculature, and altered neuronal function.
We are examining arsenic’s effects on development and cellular differentiation in several different stem cell lines and in killifish embryos using a variety of molecular (microarrays, qPCR, ChiP), epigenetic (miR expression, histone modification) biochemical and immunohistochemical techniques.
Professional organizations and scientific meetings typically attending by laboratory members include the Society of Toxicology, the Society of Environmental Toxicology and Chemistry, and Pollutant Responses in Marine Organisms
Szymkowicz DB, Sims KC, Castro NM, Bridges WC, and Bain LJ (2017) Embryonic-only arsenic exposure in killifish (Fundulus heteroclitus) reduces growth and alters muscle IGF levels one year later, Aquatic Toxicology, 186:1-10.
Bain LJ, Liu J-T, and League RE (2016) Arsenic inhibits stem cell differentiation by altering the interplay between the Wnt3a and Notch signaling pathways, Toxicology Reports, 3:405-413.
Wojyoldo JV, Vogelbeing, W, Bain LJ, and Rice CD (2016) AhR-related activities in a Creosote-adapted population of adult Atlantic killifish, Fundulus heteroclitus, two decades post-EPA Superfund status at the Atlantic Wood site, Portsmouth, VA USA, Aquatic Toxicology, 177:74-85.
McCoy CM, Stadelman BS, Brumaghim JL, Liu J-T, and Bain LJ (2015) Arsenic and its methylated metabolites inhibit the differentiation of neural plate border specifier cells, Chemical Research in Toxicology, 28:1409-1421.
Liu J-T and Bain LJ (2014) Arsenic inhibits hedgehog signaling during P19 cell differentiation, Toxicology and Applied Pharmacology, 281:243-253.
D’Amico AR, Gibson AW, and Bain LJ (2014) Embryonic arsenic exposure reduces the number of muscle fibers in killifish (Fundulus heteroclitus), Aquatic Toxicology, 146, 196-204.
Sivils JC, Ancrum TM, and Bain LJ (2013) Loss of Mrp1 alters detoxification enzyme expression in a tissue- and hormonal-status specific manner, Journal of Applied Toxicology, 33, 766-773.
Green BR and Bain LJ (2013) Mrp2 is involved in the efflux and disposition of fosinopril, Journal of Applied Toxicology, 33:458-465.
Bain LJ “Ecological Risk Assessment and Animal Models” in the Encyclopedia of Sustainability Science and Technology: Environmental Toxicology (2012) ed, E. Laws, ISBN: 1461457637, pp. 181-202.
Hong G-M and Bain LJ (2012) Arsenic exposure inhibits myogenesis and neurogenesis in P19 stem cells through repression of the beta-catenin signaling pathway, Toxicological Sciences, 129:146-156.
Hong G-M and Bain LJ (2012) Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1, Toxicology and Applied Pharmacology, 260:250-259.
Gaworecki KM, Chapman RW, Neely MG, D’Amico AR, and Bain LJ (2012) Arsenic exposure in killifish during embryogenesis alters muscle development and structure, Toxicological Sciences, 125:522-531.
Steffens AA, Hong G-M, and Bain LJ (2011) Sodium arsenite delays the differentiation of C2C12 mouse myoblast cells and alters methylation patterns on the transcription factor myogenin, Toxicology and Applied Pharmacology, 250: 154-161.
Ordonez C, Lougheed VL, Gardea-Torresdey JL, and Bain LJ (2011) Impact of metals on macroinvertebrate assemblages in the Forgotten Stretch of the Rio Grande, Archives of Environmental Contamination and Toxicology, 60: 426-436.
Gonzalez HO, Hu J, Gaworecki KM, Roling JA, Baldwin WS, Gardea-Torresdey JL, and Bain LJ (2010) Dose-responsive gene expression changes in juvenile and adult mummichogs (Fundulus heteroclitus) after arsenic exposure, Marine Environmental Research, 70:133-141.
Sivils JC, Gonzalez I, and Bain LJ (2010) Mice lacking MRP1 have reduced testicular steroid hormone levels and alterations in steroid biosynthetic enzymes, General and Comparative Endocrinology, 167:51-59.
LinksSupport for reducing the amount of arsenic in apple juice
Using killifish as a model for developmental toxicity
Clemson scientist receives $367K grant to trace how arsenic disrupts developing cells