Fluorescent

Now, read through the following descriptions and determine which fits your needs best.

  • If you need to obtain 3D information on a sample that is 5-30 microns thick with very birght staining then you will need to use Confocal imaging methods.

    Confocal

    Image of neon green and yellow butterfly proboscis on a black background

  • If your sample is relatively thin (5-20 microns), not too opaque, with only light staining, and you want to obtain 3D information then you need to use Deconvolution.

    Deconvolution

  • If you need to obtain 3D information on a think sample (greater then 30 microns thick, e.g. entire embryo, whole tissue, brain slice, etc...), if you need very high z-resolution, or if you are using UV-excitable dye in live cells then you will need to use Multiphoton.

    Multiphoton

  • If you need 2D information on a thin sample (less than 5 microns thick) you should use Widefield.

    Widefield

    Image of a brown and yellow-green butterfly proboscis on a black background