Cell, Tissue and Molecular Analysis (CTMA) core instruments

Dr. Dan Simionescu, Core Director

1) Biological safety cabinet 1300 Series Class II, type A2 , Thermo Scientific 1. Biological safety cabinet 1300 Series Class II, type A2 , Thermo Scientific

Application:

  • sterile cell culture work

Features:

  • UV light with safety lockout
  • HEPA filters in the supply and exhaust airflow
  • Constant downflow avoids cross-contamination
  • Digital Airflow Verification with visual and audible alarm
  • Internal outlets and service valves in each side

Laboratory CO2 Incubator, Isotemp, Fisher Scientific2. Laboratory CO2 Incubator, Isotemp, Fisher Scientific

Application:

  • sterile cell culture work

Features:

  • Water-jacketed, 6.5 cu ft capacity chamber
  • Temperature setpoint range 10oC – 55oC
  • Provide stable temperature, humidity and CO2 level
  • Low and high CO2, power interruption and temperature deviation alarms
  • Microbiological filters protect gas inlet and sample port
  • Easy-to-use display

Refrigerated centrifuge, Sorvall ST 40R, Thermo Scientific3. Refrigerated centrifuge, Sorvall ST 40R, Thermo Scientific

Applications:

  • Cell culture
  • Primary cell isolation

Features:

  • TX-750 4x750ml swinging bucket rotor
  • Holds 15ml and 50ml tubes
  • Max. speed 4700 RPM
  • Temperature range: -10oC to +40oC
  • User-friendly control panel to pre-set speed, running time, temperature, running profile

Digital control water bath, Isotemp 210, Fisher Scientific 4. Digital control water bath, Isotemp 210, Fisher Scientific

Application:

  • suitable for clinical, pharmaceutical or biomedical procedures

Features:

  • 10L capacity
  • Temperature range: ambient to 100oC
  • Temperature resolution: ±0.1°C
  • Temperature stability: ±0.5°C
  • Easy to remove cover for larger bottles
  • Drain pump to empty reservoir

Benchtop Incubator shaker, Excella E24, Eppendorf5. Benchtop Incubator shaker, Excella E24, Eppendorf

Applications:

  • Cell and tissue cultures
  • Microbiology
  • Bacteriology 

Features:

  • Clear large lid for excellent visibility
  • Temperature range: 7°C above ambient to 60°C
  • Shaking speed: 50 – 400 rpm; orbit: 1.9cm
  • Continuous or timed (0.1-99.9h) run
  • Digital keypad control and LED display for speed, run time, temperature
  • Alarms: open lid, 5 rpm speed or 1°C temperature deviation

Scepter 2.0 handheld automated cell counter, Millipore6. Scepter 2.0  handheld automated cell counter, Millipore

Applications:

  • Cell counting
  • Cell health monitoring
  • Cell behavoir monitoring

Features:

  • Cell size range: 6-36 mm
  • Concentration range: 10000–500000 cell/ml
  • Processing time: 30s
  • Screen displays cell concentration, average cell size and volume, histogram of size or volume distribution
  • Stores 72 histograms, USB cable port for data retrieving

Guava Easy Cyte5 Flow Cytometer, Millipore7. Guava Easy Cyte5 Flow Cytometer, Millipore

Applications:

  • Cell counting
  • Immunophenotyping
  • Cell signaling
  • Cell health and toxicity screening
  • Cell viability, cell cycle assays

Features:

  • A microcapillary flow cytometry system
  • Easy to operate, uses small sample volume (50ml)
  • Excitation laser: Blue (488nm)
  • Emission filters: Red1 (690/50nm), Green (525/30nm), Yellow (583/26nm)
  • 2 scatters: Forward and Side

Vacuum infiltration processor, Tissue-Tek VIP5, Sakura8. Vacuum infiltration processor, Tissue-Tek VIP5, Sakura

Application:

  • suitable for a variety of human, animal, or plant tissue specimens

Features:

  • Automatic tissue processor, holds up to 150 tissue cassettes or up to 300 cassettes
  • Holds 15 bottles (10 for processing reagents, 3 for cleaning cycle and 2 for waste)
  • VIP software is programmable for up to twenty different programs for use in the fixation, dehydration, clearing, and paraffin infiltration
  • Delay mode allows programming a desired end time (the program automatically calculates the start time)

Paraffin embedding workstation, HistoStar, Thermo Scientific9. Paraffin embedding workstation, HistoStar, Thermo Scientific

Application:

  • Embedding routine tissue samples into paraffin blocks for microtome sectioning  

Features:

  • Brightly illuminated and clutter free LED lighting for uniform illumination of the workplace
  • Smooth and insulated contact points
  • 5L paraffin capacity, coldplate area for 72 base molds, large heated workspace and specimen holding area
  • Adjustable paraffin dispense paddle
  • Heated wax trimmer built into the workspace
  • Large, easy-to-read touchscreen display control

Automated cryostat, Leica CM1950, Leica Biosystems10. Automated cryostat, Leica CM1950, Leica Biosystems

Application:

  • sectioning  frozen tissues

Features:

  • -35oC cryochamber with 15 freezing stations
  • Motorized sectioning with slow (300mm/s in 20mm steps) or fast (900mm/s) electric coarse feed
  • Section thickness range: 1 to 100mm
  • Automatic cryochamber defrosting
  • Control panels comfortably positioned in the armrests
  • Built-in UVC disinfection system and  AgProtect antimicrobial nanosilver coating on the outside surfaces
  • Section Waste Removal system with a series of threefilters (including HEPA)

Automated rotary microtome, Leica RM2255, Leica Biosystems11. Automated rotary microtome, Leica RM2255, Leica Biosystems

Application:

  • sectioning paraffin and resin embedded specimens

Features:

  • Allows motorized as well as manual sectioning
  • Motorized sectioning with slow (300mm/s) or fast (900mm/s) electric coarse feed
  • Section thickness range: 0.5 to 100mm
  • Precise specimen orientation with zero point reference
  • Intuitive control panel
  • Spacious and easy-to-clean section waste tray

BondMax automated IHC/ISH staining system , Leica Biosystems12. BondMax automated IHC/ISH staining system , Leica Biosystems

Applications:

  • immunohistochemistry (IHC)
  • In situ hybridization (ISH)

Features:

  • Fully automation ensures the same high-quality staining each time
  • Ready to use reagents eliminate preparation work
  • Three independent slide trays ensure flexibility and continuous staining
  • Stains more slides ( total capacity of 30) in less time offering greater efficiency
  • Trays keep slides organized, not random

Automated glass coverslipper, Leica CV5030, Leica Biosystems13. Automated glass coverslipper, Leica CV5030, Leica Biosystems

Applications:

  • Histology sections
  • Cytology smears
  • Monolayer preparations

Features:

  • Optional wet or dry coverslipping, with approximately 400 slides/hour speed
  • The load vessel accepts up to 60 samples
  • Accepts different sizes of coverglass, automatically discarding broken ones
  • Easy-to-use control panel enables settings to be customized
  • 250ml mounting media bottle (accepts any commercially available media)
  • Integrated fume extraction system with activated charcoal filter

PXC-200 Analytical balance, Intell-Lab14. PXC-200 Analytical balance, Intell-Lab

Application:

  • measuring dry components of solutions used in cell culture, biochemistry and molecular biology

Features:

  • Three door glass draft shield
  • Internal calibration
  • Large backlit LCD display
  • Capacity: 200g
  • Resolution: 0.0001g (0.1mg)

Milli-Q Direct8 water purification system, Millipore15. Milli-Q Direct8 water purification system, Millipore

Applicationa:

  • Pure water for everyday laboratory work (dishwashing, cleaning, etc)
  • Ultrapure water for any cell culture, biochemistry and molecular Biology procedure

Features:

  • Provides pure and ultrapure water directly from tap water
  • 60L storage tank for the pure water
  • Ultrapure water: resistivity 18.2 MΩ•cm, TOC<5ppb
  • Built-in 185nm UV lamp for TOC reduction
  • Intuitive screen commands for operation, maintenance and

AB15 digital pH meter, Fisher Scientific16. AB15 digital pH meter, Fisher Scientific

Application:

  • optimizing pH of solutions used in cell culture, biochemistry and molecular biology

Features:

  • Measures pH and temperature
  • pH range: -1.99 to 19.99, resolution: 0.1/001, accuracy: ±0.0
  • Temp. range: -5 to 105oC, resolution: 0.1oC, accuracy: ±0.5oC
  • Large LCD display with easy to follow instructions

5417C Microcentrifuge, Eppendorf17. 5417C Microcentrifuge, Eppendorf

Applications:

  • Protein isolation
  • Nucleic acid isolation

Features:

  • Fixed angle rotor
  • Max. speed: 14000RPM
  • Max. capacity: 30 x 1.5 or 2ml tubes
  • Timer: 0 to 99 min. in 1min. increment
  • Backlit, full-digit display for easy reading

Mini-Protean 3 electrophoresis system, Bio-Rad18. Mini-Protean 3 electrophoresis system, Bio-Rad

Applications:

  • SDS-PAGE protein separation
  • Nucleic acid PAGE separation

Features:

  • Accepts hand cast and precasted BioRad gels
  • Runs one or two gels simultaneously
  • Additional blot module for performing the transfer
  • Universal power supply

Epoch microplate spectrophotometer, BioTek19. Epoch microplate spectrophotometer, BioTek

Applications:

  • Colorimetric Elisa
  • Protein quantification
  • Nucleid acid (ssDNA, dsDNA, RNA) quantification

Features:

  • Wavelength scanning, end point, kinetic and well area scanning
  • Accommodates standard 6-,12-, 24-,48-,96- and 384 well microplates
  • Wavelength range 200 to 900nm
  • Intuitive and user-friendly Gen5 analysis software
  • Special Take3 multi-volume plate for very low volume (2ml) of samples

ChemiDoc It2 Imager, UVP20. ChemiDoc It2 Imager, UVP

Applications:

  • Protein gel
  • DNA gel
  • Chemiluminescent blot

Features:

  • Image capture, quantitative analysis of chemiluminescent blots and gels
  • Transilluminator on a sliding tray for easy sample placement
  • Epi White Lights built into the top for lighting and focusing
  • Four-position filter wheel, with included ethidium-bromide one
  • LED white light plate for Coomassie blue and silver staining
  • VisionWorksLS Image Acquisition and Analysis software

Rotor-Gene Q real-time PCR cycler, Qiagen21. Rotor-Gene Q real-time PCR cycler, Qiagen

Applications:

  • Gene expression analysis
  • Genotyping
  • Pathogen detection
  • Methylation analysis

Features:

  • Enables high-precision real-time PCR, end point PCR, and high resolution melt analysis
  • Unique rotary format (36 or 72 well rotor) for thermal and optical uniformity
  • 5 available channels: blue, gree, yellow, orange, red and crimson
  • The comprehensive Rotor-Gene Q software supports all current real-time analysis procedures