Dr. Robert Latour, Core Director, Supervisor
Dr. Gulya Korneva(firstname.lastname@example.org), Lab Support, 864-656-5134
Bioactive Matrix Synthesis and Bioreactor Design:
Dr. Ken Webb, Supervisor
Dr. Gulya Korneva, Lab Support
Dr. Jiro Nagatomi, Supervisor
Dr. Gulya Korneva, Lab Support
The overarching theme of SC BioCRAFT is regenerative medicine, a highly interdisciplinary field; expertise and support in bioengineering methods as well as cell and molecular biology are required to succeed. Junior investigators in our IDeA state need core support to further their research and compete nationally for grant funding. From our experience in Phase I and from several discussions with our internal advisory committee and targeted PIs, we have identified four bioengineering thrust areas that PIs will need core support in. To meet these needs, the previously named “Materials Synthesis, Characterization, and Testing Core” has been expanded and renamed the “Bioengineering and Bioimaging Core (B5)”. This is the only bioengineering core in the COBRE programs throughout the US (out of > 200 cores). This Core will provide support for both COBRE and outside investigators for their research needs under the areas of Biomaterials characterization, Bioactive matrix synthesis and Bioreactor design, Biomechanics, and in vivo Bioimaging. The core will maintain a broad range of instruments and provide technical support and training for facilities for each of these areas.
Clemson University has significantly invested in developing this core in Phase I, including the addition of new laboratory space and advance instruments at Clemson’s main campus and at Clemson University Biomedical Engineering Innovation Campus (CUBEInC) in nearby Greenville, SC, and addition of faculty with specific expertise in different thrust areas of this core that include targeted investigators in Phase I.
The overall goal of the Bioengineering and Bioimaging Core is to provide resources, facilities, and training to support research related to tissue engineering, regenerative medicine, and drug delivery-system design for designated COBRE PIs and other research groups within and outside the State of South Carolina to increase the state’s competiveness in biomedical research. This goal will be achieved through four specific aims:
Instruments at the BB Lab:
Spectropolarimeter (Jasco J-810)
|Temperature Controlled Automatic Refractometer AR700 (Reichert)
Specifications: Reading range of 1.33000 to 1.60000 Refractive Index and the full Brix scale. Precision: to 0.01 Brix or 0.00001 RI
Applications: Measures refractive index of transparent, translucent, and opaque liquids.
|Contact angle goniometer DSA-20E (Krüss)
|Variable-angle Spectroscopic Ellipsometer GES 5 (Sopra Inc)
Features: Thickness measured: from monoatomic to several microns thick. The beam size: 1 mm2 and 10 mm2; The variable angles: from 7º to 90º with variable +/- 0.01º; spectral range from 200 nm to 800 nm
|Flame Atomic Absorption Spectrophotometer - AAnalyst 200 Instrument (Perkin Elmer)
Specifications: Wavelength range from 189 to 900 nm.
Applications: For determining very precisely the concentration of a particular element in a liquid sample. Can detect from 1 to 1000 µg/L. For each element, the specific lamp is required. Ca, Cu, and Fe lamps are available. The list of lamps/elements for analysis on AAnalyst 200 can be found here: VWR
|Surface Plasmon Resonance Spectrometer Biacore X
Biacore is a methodology for measuring the interactions mostly between proteins, in real time using the phenomenon of surface Plasmon resonance (SPR).
|Materials Testing System (MTS) Synergie 100
Applications: Tensile, Flex, Peel/Tear, Strain, and Compression test methods.
|HPLC/GPC System (Waters)
Applications: MW determination, high resolution separation and purification.
Features: Binary Gradient Pumping System, Autosampler, Photodiode Array Detector (Wavelength Range from 190 to 800 nm with 1.2 nm resolution with wavelength accuracy: ± 1 nm); Refractive Index Detector (Refractive Index Range: 1.00 to 1.75 RIU with temperature control from 30 to 55oC); Multi Lambda Fluorescence Detector (Operates from 200 to 900 nm); Column Heater (Temperature Range from 20oC to 60oC; with temperature accuracy of ±0.8oC; Column Capacity of up to four 7.8 mm x 300 mm columns); Fraction Collector (Methods of Operation: time, drop count, drop volume, signal).
|Expanded Plasma Surface Treatment Unit PDC-001 (Harrick Plasma)
Dr. Rick Visconti
Dr. Dan Simionescu
Dr. Shibnath Ghatak
Histology and Advanced Imaging:
Dr. Terri Bruce, Co-Director
Dr. Bruce Gao
Stem Cell Biology:
Dr. Carol Brenner, Co-Director
Dr. Dan Simionescu
Cell, tissue, and molecular biology techniques are the cornerstone of biomedical research and regenerative medicine endeavors. A major obstacle for junior faculty is lack of state-of-the-art facilities and specialized expertise commonly found in states with major NIH funding. This often hinders the generation of preliminary data necessary to be competitive for NIH R01 grants. In the State of South Carolina, there are several strong facilities and successful investigators who have received generous NIH funding and have gained significant tissue, cell, and molecular expertise. However, junior faculty in SC have not benefited optimally from these opportunities. To close this gap, we developed the Cell, Tissue and Molecular Analysis Core (CTMA core) to be affiliated with SC BioCRAFT.
After conferring with the PI of the COBRE proposal and the five targeted PIs, we have identified three thrusts that will serve the needs of all our COBRE projects: 1) molecular biology, 2) histology and advanced imaging, and 3) stem cell biology. Outstanding facilities and labs directed by expert investigators in these three thrusts have been identified and brought together to serve the needs of COBRE PIs and those of other investigators in the State of South Carolina in CTMA core. Areas of expertise in this core include regenerative medicine, gene and protein expression and manipulation, adult and induced stem cells, and advanced histology, electron microscopy, and biophotonics. The Core will provide support to develop ideas, overcome technical challenges, and lead the targeted projects towards significant outcomes as outlined in the following specific aims: