Bioengineering

Faculty Directory

Anthony Guiseppi-Elie, ScD

Prof. of Bioengineering and Director of the Center
for Bioelectronics, Biosensors, and Biochips

B.Sc. Hons. First Class, 1979 University

of the West Indies, Jamaica
M.Sc., 1980 Univ. of Manchester Institute of Science

and Technology, England
Sc.D. Polymer Materials Science and Engineering (III),

1983 Massachusetts Institute of Technology

Research Interests

Biomedical Biosensors for Hemorrhagic Shock
Prognostic & Diagnostic DNA Biochips for Cancer
Whole Cell Biosensors for Neurotoxicity Testing

Contact

E mail:aguisep@clemson.edu
Office: 132 Earle Hall
Phone: 864.656.1712

C3B Laboratories ...more

Honors, Awards and Professional Activities

Keynote address: 2005 Annual General Meeting of the Virginia Space Grant Consortium
Keynote address: 2005 Annual General Meeting of the Richmond Joint Engineers Council
Invited Lecturer in Nanobiotechnology (Apr 2004), Technology Forum on Nanobiotechnology,

US Patent and Trademark Office (USPTO), Washington DC, USA. Distinguished Lecture in Bioengineering (Mar 2004), National Security Agency and Univ.

of Maryland Distinguished Lecture in Pioneering Biomedical Research (Oct 2003), Purdue University
Distinguished Lecture in Polymer Research (Oct 2003), Program in Polymer Science and

Technology (PPST), Massachusetts Institute of Technology. 1999 SEAM Award “… for taking conductive polymers to biological applications”. Herman

Mark Polymer Institute, Polytechnic University, Brooklyn, New York. Visiting Scientist (06/1995 - 06/1996): Department of Biomedical Engineering, School of

Medicine, Johns Hopkins University Adjunct Professor of Materials Science (09/1993 - 06/1995): Department of Materials Science,

Polymer Science Program, Penn State University Invited Lecturer: The First Asia -Pacific Symposium on Biosensors; University of Wollongong:

Australia; December 4 - 6, 1995. Plenary Lecturer: The XIth Conference of Chemistry and Chemical Engineering; University of

the West Indies, St. Augustine: Republic of Trinidad and Tobago; March 6 - 10, 1995 Symposium Chairman, Symposium on Transducer-active Polymers, ACS National Meeting, 1994

Co-chairman, Symposium on Field Responsive Polymers, 35th IUPAC Macro Meeting, Akron

Ohio, 1994.
Senior Fellow, Center for the Study of Biological Complexity, Virginia Commonwealth Univ.
Fellow, American Institute of Chemists
Life Member, MIT Chapter Sigma Xi.
Commonwealth Postgraduate Scholarship Award.
UWI Postgraduate Scholarship Award.
UWI Undergraduate Bursary Award for academic merit.
Member, Editorial Advisory Board, Journal of Bioactive and Compatible Polymers (2002-Current);

SAGE Publications Founding Member, Editorial Advisory Board, NanoBiotechnology (2004 - Current); Humana Press
Member, Editorial Advisory Board, Your World: Biotechnology and You (2000 - Current);

Biotechnology Institute Member NIH NIBIB Study Section on Biomaterials and Biointerfaces

(2004 – current).
NSF EGRC, NSF IUCRC, NSF NSEC and NSF IGERT site reviewer.
Member EPSCoR Foundation Board (2006 – current)
Founder, President and Scientific Director, ABTECH Scientific, Inc. (1995 - current)

Current Research

Implantable Biosensors for in-vivo Monitoring of Glucose & Lactate Related to Trauma

Trauma that leads to hemorrhage results in poor peripheral perfusion, i.e., comprimised blood flow to the tissues, vasoconstriction, tissue hypoxia (depleted oxygen levels), hyperlactatemia (elevated lactic acid levels) and eventually hemorrhagic shock, a condition that results in multiple organ dysfunction syndrome (MODS). Our center is engaged in the development of the PSMBioChip (physiologic status monitoring biochip), an implantable biosensor system that can measure and remotely report on intramuscular or subcutaneous levels of lactate and glucose during hemorrhage. Present studies use a hemorrhage rat model and explore the development of the bioactive smart biorecognition layers for the biosensor, the integration of the miniaturized instrumentation, and the wireless communication between sensor and base station. Useful for severely injured soldiers and civilian trauma victims, this device promises to aid in the development of new resuscitation strategies.

Gene Expression Profiling of Primary Brain Tumors & Development of DNA Biochips

There are approximately 17,000 brain cancer victims in the United States each year. Current pathological methods for classifying the grades and stages of these tumors are subjective and prone to misdiagnosis. Experiments are being conducted using DNA microarrays to identify targeted suites of genes associated with astrocytomas. These genes will be used as probes in a brain tumor biochip. Such a chip will delineate that the World Health Organization has defined as classes of astrocytomas for improved diagnostics and will lead to imprived therapeutic efficacy. DNA biochips have the potential to revolutionize the way cancer tumors are classified and patients are stratified for targeted therapies.

Whole-Cell Neurotoxicity Biosensor as an Alternative to the Use of Animals

Cell-based neurotoxicity array biosensors increase testing throughput by providing multiple wells integrated with cell-based biosensing elements. By using an eight-well array system of differentiated neuronal cells, scientists can test for the effects of a toxin on eight differentiated cell cultures or eight toxins on a given cell culture. This platform project also offers researchers an alternative to using live animals in their investigations by providing differentiated cell cultures as proxies for living tissues.

Recent Publications

Anthony Guiseppi-Elie, Scott Taylor, Louise Lingerfelt, Chris Nixon, Ryan Georgiana, Joy Kim, Stephanie Smith, Brad Mangrum and Nicholas Farell “Studies of the Interaction of Platinum Drugs with DNA Using Oligonucleotide Microarrays” Macromolecular Symposia (2006) 235(1), 115-120.

Anthony Guiseppi-Elie, “Bioactive Hydrogels” Materials Matters 2006, 1(1), 8.

Amy Yu, Tim Savas, G. Scott Taylor, Anthony Guiseppi-Elie, Henry I. Smith, Francesco Stellacci “Supramolecular Nano Contact-Printing: using DNA as a moveable type.” Nano Letters (2005), 5(6), 1061-1064.

Anthony Guiseppi-Elie, Sean Brahim, Gymama Slaughter and Kevin R. Ward “Design of a Subcutaneous Implantable Biochip for Monitoring of Glucose and Lactate” IEEE Sensors Journal (2005), 5(3), 345-355.

Sheena Abraham, Sean Brahim, Kazuhiko Ishihara and Anthony Guiseppi-Elie “Molecularly engineered hydrogels for implant biocompatibility” Biomaterials (2005), 26(23), 4767-4778.

Department of Bioengineering
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