Mini-Workshops
These hands-on mini-workshops focus on experimental techniques used in biomaterials research such as hard tissue histotechnology, immunohistochemistry, surface spectroscopy, tissue procurement for cell culture, bioinstrumentation, and confocal microscopy.
Mini-Workshop 1: Introduction to Biomaterials
Introductory workshop providing a comprehensive overview of materials used in the medical field. Classic material constructs in orthopaedics, vascular and dental applications will be covered, and attention will be given to modern formulations of metals, ceramics, and polymers for use in regenerative medicine. Topics of materials selection, implant design, and mechanical testing will be addressed. In addition, physical and mechanical properties of tissue as related to microstructure and issues of corrosion and wear in the body will be investigated.
Mini-Workshop 2: Cell and Tissue Culture
Methods for studying cell structure and function including basic cell culture techniques and in vitro biocompatibility analyses; tissue procurement techniques and cell isolation. Cell count and different types of assays used to evaluate cell response; principles and enzyme identification; spectrofluorometry.
Mini-Workshop 3: Hard Tissue Histology and Immunohistochemistry
Techniques used for the processing of implants and surrounding tissue; plastic resin selection, embedding, sectioning, grinding, and staining protocols specifics to plastic embedded sections (EXAKT system, Buhler instruments). Techniques; monoclonal and polyclonal antibody preparations; enzyme selection for specific tissues; data interpretation. Slide libraries (includes a microscope tutorial).
Mini-Workshop 4: Surface Spectroscopy
Principles of chemical characterization of surfaces. Sample handling; data acquisition; data library. Spectrum analysis (Fourier transformed infra-red spectroscopy, x-ray photoelectron spectroscopy, UV-VIS spectroscopy).
Mini-Workshops 5 and 6: Bioinstrumentation and Confocal Microscopy
This workshop will provide its participants with a hands-on experience into the field of human electronics interfaces and medical imaging technology. Basic lectures on circuit design and instrumentation will be applied in bioinstrumentation workshops that will focus on the acquisition and monitoring of human vital signs. In addition, participants will explore the field of medical imaging and learn about recent advances in health care diagnosis and monitoring of human systems. The second day will focus on fluorescence and confocal microscopy techniques for cell culture analysis.
Department of Bioengineering
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