A biosensor is an analytical device constructed by incorporating an analyte specific bioelement (which creates a recognition event) and a sensing element (which transduces the recognition event).
My project is development and modification of electrochemical biosensor. Porous glassy carbon electrode is used as a platform for bioassays. High surface area, high void volume, low background current make porous carbon electrode advantageous over glassy carbon electrode.
The goal of the project is to develop a DNA biosensor using avidin-biotin chemistry with Hydroquinone diphosphate (HQDP) as a substrate, and modification of electrode to reduce non specific binding of protein and nucleic acid. DNA molecules are immobilized on modified electrode surface using avidin biotin coupling. Target DNA molecule is sandwich between immobilized DNA strand and enzyme linked biotinylated DNA strand. The analyte is then detected by enzymatic conversion of HQDP to HQ (hydroquinone) which is detected by cyclic voltametry