CURF Home>>Technology Showcase >>Stabilizing Multi-Well Plate Insert for Biomaterial Testing>>Existing Method

Existing Approaches and Their Drawbacks:

Summary of ASTM F813

  1. Cells grown to confluent monolayer
  2. Biomaterial sample placed on top
  3. Incubate for 24 hours
  4. Evaluate cells underneath or beside material

Limitations of ASTM F 813 which hinder detailed biocompatibility analysis

Problem
Remedy
Effect
Mechanical damage to cell monolayer caused by crushing or sliding of material None
  • Test not measuring material properties
  • Skews quantitative assays
Cells underneath have no access to media None
  • Partitioning effect skews quantitative assays
Loose contact between cells and material surfaces None
  • Test not measuring material properties
Material may float Glues or weights
  • Introduces foreign substance
  • Awkward

Summary of Alternative Method

  1. Biomaterial sample placed in well
  2. Cells seeded on top in minimum volume and allowed to adhere; additional media added
  3. Incubate
  4. Evaluate cells

Limitations of Alternative Method which hinder detailed biocompatibility analysis

Problem
Remedy
Effect
Material may float Glues or weights
  • Introduces foreign substance
  • Awkward
  Flip material and culture cells upside down
  • It is difficult to assess the real consequences of upside down cell culture

Cells may migrate around / underneath material:

  • Crushing / sliding damage to cell monolayer
  • Multiple cell / material interfaces
 Anti-adhesive coatings (BSA)
  • Additional procedure
  • Introduces additional variables
  • BSA hinders only attachment to well plate, cells may still migrate on material

 

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