Bio-Rad GS-710 Imaging Densitometer

Imaging densitometer with 12-bit precision, 42 micron scan resolution, 3 optical density linear dynamic range and variable wavelength light source from 400 to 700 nm. The Model GS-710 had an internal calibration strip for accurate quantification of optical density. Associated with the densitometer a personal computer, Quantity One® image analysis software and a SCSI interface. The system allows for transmittance and reflectance modes of measurement to suit many different types of samples. The Model GS-710 imaging densitometer is for high sensitivity imaging. With red, green, and blue color CCD technology, scanning can be done with almost any sample (1-D and 2D gels, dot and slot blots, autoradiograms, slides, and photographs) at the proper wavelengths for greater sensitivity and accuracy. Scans can even be done of red-colored samples without the sensitivity problems found with laser densitometers. Scanning area is 20 x 25 cm.

Quality One®; is a powerful, flexible software package for imaging and analyzing 1-D electrophoresis gels, dot blots, slots blots and colonies. The software has an easy to use graphical interface with standard pull-down menus, toolbars, and keyboard commands. Quantity One can quantitate and analyze a wide variety of biological data, including radioactive, chemiluminescent,.fluorescent and color-stained samples acquired form densitometers, phosphor imagers, fluorescent imagers and gel documentation systems. The lane based functions can be used to calculate molecular weights, isoelectric points, VNTRs, Rf values, and other values. The software can measure total and average quantities, determine relative and actual amounts of protein, and count colonies in a Petri dish. The software can cope with distortions in the shape of lances and bands. Lances can be adjusted along their lengths to compensate for any curvature or smiling of gels. Irregularly shaped, non-lane-based data can be accurately quantitated using the volume tools.

Data captured by the imaging device are stored in a file under a user- defined name. Files can be easily converted into TIFF format for compatibility with other Macintosh and Windows applications.

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