Hewlett Packard (Agilent Technologies) High Pressure Liquid Chromatograph

High pressure liquid chromatography (HPLC) is an analytical separation technique with a wide variety of separation modes to choose from based on the stationary phase or column. Separation phases can be classified according to the mechanism by which they separate molecules: partition phases, adsorption phases, ion exchanges phases and size exclusion phases. The most common column material is reverse phase, separating by partition and some adsorption by non-protected silanol groups, with the analytes retained on the less polar stationary phase until eluted with a sufficiently polar mobile phase. Often the polar phase is primarily water which reduces the cost of solvents used for the analysis. The 1090 Hewlett Packard HPLC located in the Multi-User Analytical laboratory has a tertiary solvent system (3 solvent pumping capacity). The flow rate is dependent on the column and packing used. When using a 3 µm C18 packing material in a 10 cm x 4.5 mm column the flow rate is generally 0.5 ml/min. The detectors available on the HPLC system are a diode array detector (190-600 nm) with the ability to obtain spectra during the analysis run and a fluorescence detector. The system has an autosampler with 100 sample capacity which allows for 24 hour/day operation. The system is controlled by HP ChemStation software on a personal computer and has a LaserJet printer