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Tissue culture study seeks optimum storage conditions
A study of light and temperature levels is seeking to determine the optimum storage conditions and acclimatization techniques for tissue culture propagation. By understanding the process of storage and acclimatization, greenhouse growers can extend the time that plants are available for sale.

The study is using tissue culture samples, called micropropagules, of several Hosta varieties to test their response to varying levels of light and temperature, as well as liquid sucrose, during storage. After a storage period of 2 – 6 months, researchers will measure plantlet growth, photosynthetic characteristics, and the amount of soluble sugars and starch in shoots and roots. The plantlets will then be transplanted and placed in the greenhouse to investigate methods for improving acclimatization before sale. 
(For more information, contact Nihal Rajapakse nrjpks@clemson.edu, Clemson Horticulture Department.)  

Another tissue culture study is being conducted using medicinal plants in a system that was developed and patented by Clemson scientists in 1998. This study is growing four medicinal plant species in the patented system as a way to increase production levels and standardize chemical contents.
(For more information, contact Jeff Adelberg jadlbrg@clemson.edu, Clemson Horticulture Department.)

Technical articles:
Nutrient transfer in a thin film system
(pdf 67 KB)

Capillary Surface Materials fibers in potting mix
(pdf 159 KB)