Mass Spectrometers

Listed below are the current major instrumentation in MUAL, along with key specifications and primary application.

Liquid Chromatography- Mass Spectrometry

Thermo Orbitrap Fusion™ Tribrid™ Mass Spectrometer

Thermo Orbitrap Fusion Tribrid Mass Spectrometer
  • Combines quadrupole, ion trap and Orbitrap mass analysis in a revolutionary Tribrid architecture
  • Ultrahigh resolution up to 500,000 FWHM
  • Sub-1-ppm mass accuracy
  • Multiple dissociation techniques—CID, HCD, ETD
  • ID-X capability for small molecule analysis
  • Coupled to ESI and nano-ESI interface
  • UltiMate 3000 RS UHPLC & UltiMate 3000 RSLCnano
  • Primary application: Global metabolomics, Proteomics, Characterization of unknowns

Shimadzu LCMS-8040

Shimadzu LCMS-8030 instrument
  • Triplequadrupole mass spectrometer
  • Fast scan speeds with ultrafast polarity switching
  • 15,000 u/second with a 0.1 Dalton step width without the loss of mass accuracy.
  • Redesigned pseudo-quadrupole collision cell, allows for 1msec pause times to eliminate crosstalk
  • Coupled to ESI interface

Waters Xevo TQ-XS

Waters Xevo TQ-XS
  • Triple quadrupole mass spectrometer
  • Equipped with StepWave XS™ ion guide to enhance sensitivity, T-Wave and ScanWave Collision Cell for rapid simultaneous MS/MS and MS scanning, and Xtended Dynamic Range (XDR) photomultiplier detector allows for larger ranges of linearity.
  • Ultrafast scan speeds (20,000 Da/second) with rapid polarity switching.
  • Coupled to ESI interface
  • MRM tuning with ESI infusion for unmatched sensitivity
  • Primary application: Trace analysis, targeted metabolomics, Quantitative proteomics, Quantitative analysis

Gas Chromatography- Mass Spectrometry

Agilent 7250 GC/QTOF

Agilent 7250 GC/QTOF instrument
  • Quadrupole Time-of-flight mass spectrometer
  • Delivers full-spectrum, high-resolution, accurate-mass data
  • Low-energy EI for softer ionization and molecular ion enhancement
  • Elucidates chemical structures with MS/MS capabilities
  • TOF mass accuracy- < 2ppm RMS
  • TOF Resolution > 25,000 at m/z 271.896
  • Data acquisition rates of up to 50 spectra per second
  • Electron Ionization, settable 5-200 eV
  • Primary application: Metabolomics, Characterization & quantitation of GC-amenable compounds

Agilent 5975C Series GC/MSD System

Agilent 5975C Series GC/MSD System
  • Single quadrupole mass spectrometer
  • Standard Electron Ionization
  • Triple-Axis Detector for superior sensitivity and spectral integrity
  • Coupled to a Pyroprobe 5150 Pyrolyzer
  • Can sample solid samples
  • Ambient to 1400°C and programmable in 1°C increments
  • Cryofocusing to produces sharper peaks
  • Primary application: Metabolomics, Characterization & quantitation of GC-amenable compounds

Agilent 240 GC/MS Ion Trap System

Agilent 240 GC/MS Ion Trap System
  • Ion Trap mass spectrometer
  • Standard Electron Ionization
  • Features MS/MS and MSn capabilities
  • Coupled to a Pyroprobe 5150 Pyrolyzer
  • Selective ion trapping for higher sensitivity
  • Full spectrum scan without any ion loss
  • Off-campus
  • Primary application: Characterization & quantitation of GC-amenable small molecules


Liquid Chromatographs

Thermo UltiMate 3000 UPLC; Thermo UltiMate™ 3000 RSLCnano; Waters ACQUITY UPLC I-Class PLUS; Shimadzu UFLC

Thermo UltiMate 3000 UPLC
Thermo UltiMate 3000 RSLCnano
Shimadzu UFLC
  • Coupled to mass specs and to diode-array and florescence detectors.

Shimadzu 2010 plus, Agilent 7890B, 7890A

Shimadzu 2010 plus
Agilent 7890B
Agilent 7890A
  • Coupled to flame ionization detectors

Other Instruments

Thermo Scientific X-Series 2 ICP-MS; Astoria Pacific Nutrient Analyzer

Thermo Scientific X-Series 2 ICP-MS
Astoria Pacific Nutrient Analyzer
  • Elemental and nutrient analysis