Instrumentation

Mass Spectrometers

Listed below are the current major instrumentation in MUAL, along with key specifications and primary application.

• Combines quadrupole, ion trap and Orbitrap mass analysis in a revolutionary Tribrid architecture
• Ultrahigh resolution up to 500,000 FWHM
• Sub-1-ppm mass accuracy
• Multiple dissociation techniques—CID, HCD, ETD
• ID-X capability for small molecule analysis
• Coupled to ESI and nano-ESI interface
• UltiMate 3000 RS UHPLC & UltiMate 3000 RSLCnano

• Primary application: Global metabolomics, Proteomics, Characterization of unknowns

Shimadzu LCMS-8040

• Triplequadrupole mass spectrometer
• Fast scan speeds with ultrafast polarity switching
• 15,000 u/second with a 0.1 Dalton step width without the loss of mass accuracy.
• Redesigned pseudo-quadrupole collision cell, allows for 1msec pause times to eliminate crosstalk
• Coupled to ESI interface

Waters Xevo TQ-XS

• Triple quadrupole mass spectrometer
• Equipped with StepWave XS™ ion guide to enhance sensitivity, T-Wave and ScanWave Collision Cell for rapid simultaneous MS/MS and MS scanning, and Xtended Dynamic Range (XDR) photomultiplier detector allows for larger ranges of linearity.
• Ultrafast scan speeds (20,000 Da/second) with rapid polarity switching.
• Coupled to ESI interface
• MRM tuning with ESI infusion for unmatched sensitivity

• Primary application: Trace analysis, targeted metabolomics, Quantitative proteomics, Quantitative analysis

Agilent 7250 GC/QTOF

• Quadrupole Time-of-flight mass spectrometer
• Delivers full-spectrum, high-resolution, accurate-mass data
• Low-energy EI for softer ionization and molecular ion enhancement
• Elucidates chemical structures with MS/MS capabilities
• TOF mass accuracy- < 2ppm RMS
• TOF Resolution > 25,000 at m/z 271.896
• Data acquisition rates of up to 50 spectra per second
• Electron Ionization, settable 5-200 eV

• Primary application: Metabolomics, Characterization & quantitation of GC-amenable compounds

Agilent 5975C Series GC/MSD System

• Single quadrupole mass spectrometer
• Standard Electron Ionization
• Triple-Axis Detector for superior sensitivity and spectral integrity
• Coupled to a Pyroprobe 5150 Pyrolyzer
• Can sample solid samples
• Ambient to 1400°C and programmable in 1°C increments
• Cryofocusing to produces sharper peaks

• Primary application: Metabolomics, Characterization & quantitation of GC-amenable compounds

Agilent 240 GC/MS Ion Trap System

• Ion Trap mass spectrometer
• Standard Electron Ionization
• Features MS/MS and MSn capabilities
• Coupled to a Pyroprobe 5150 Pyrolyzer
• Selective ion trapping for higher sensitivity
• Full spectrum scan without any ion loss
• Off-campus

• Primary application: Characterization & quantitation of GC-amenable small molecules

Chromatographs

Thermo UltiMate 3000 UPLC; Thermo UltiMate™ 3000 RSLCnano; Waters ACQUITY UPLC I-Class PLUS; Shimadzu UFLC

• Coupled to mass specs and to diode-array and florescence detectors.

Shimadzu 2010 plus, Agilent 7890B, 7890A

• Coupled to flame ionization detectors

Thermo Scientific X-Series 2 ICP-MS; Astoria Pacific Nutrient Analyzer

• Elemental and nutrient analysis