The Clemson Light Imaging Facility is housed on the ground floor of the Life Sciences Facility on the Clemson University campus. The facility contains the following state of the art light microscopes:
The Eclipse Ti is a fully motorized, perfect focus microscope. The system is fully motorized and computer controlled for six dimensional imaging. The motorized encoded Z drive has three sensitivities with a minimum step size of 25 nm. The Eclipse Ti microscope is equipped with brightfield and DIC, as well as epi-fluorescence and TIRF laser sources. A variety of objective lenses are available for the Eclipse Ti, which may be coupled with a 1.5X intermediate zoom. The Clemson Light Imaging Facility has dry, water immersion and oil immersion objective lenses ranging from 4X to 100X, including the Nikon 60X TIRF oil immersion lens with the world’s highest numerical aperture of 1.49.
The Nikon AZ100 Multizoom
The Nikon AZ100 Multizoom is a multipurpose zoom microscope with a wide range of magnification from 5X-400X. Due to its smooth zoom mechanism, a single specimen can be examined from a macro to a micro scale. The microscope is equipped with both transmitted and episcopic DIC, epifluorescence and brightfield observation capabilities.
The Eclipse Ti and the Nikon AZ100 Multizoom may be coupled to either the DS Ri-1,12mp Color Camera or the CoolSnap HQ2 High Sensitivity Quantitative Monochrome Camera for widefield imaging or to the Nikon C1si spectral imaging confocal laser scanning system for confocal or spectral imaging. The laser scanning confocal system utilizes four tunable lasers ranging from 404 to 638 nm, allowing for a wide range of fluorescence imaging. This system allows for the acquisition of 32 channels of fluorescence spectra over a 320 nm wide wavelength range in a single pass. The spectral imaging system cleanly separates overlapping spectra of different fluorescent labels and autoflourescence from true signal.
The Nikon imaging software, NIS-elements provides integrated control of the microscope and imaging systems. This software platform offers a wide range of tools for measurement, documentation and databasing. In addition, Adobe Photoshop is available for image analysis in the CLIF.
This universal design upright microscope allows users to image both “traditional” biological samples, such as thin tissue sections prepared on a slide and sealed under a coverslip, as well as larger specimens that do not fit on a slide. This feature is facilitated by the stage, which can be moved in the Z-direction, and the objectives, some of which are extra-long working distance lenses. Observation modes include transmitted and reflected light, darkfield, and fluorescence. Extended depth of focus images can be prepared using either the integrated NIS-Elements software package, or by using the 3rd party software package, Helicon Focus.
The LEXT 3D laser measuring microscope system performs non-contact roughness measurements, 3D measurements, and measurements of acute-angled specimens (slopes up to 85o). Images larger than the field of view can be collected using the stitching program. The Z-drive moves in steps less than 1 nm. Accuracy and repeatability guaranteed by Olympus.
Leica SPE Confocal
This spectral confocal features four laser lines with tunable filters. Objectives ranging from 10X to 63X are available, and the system features scan speeds ranging from 400 to 800 Hz. Other features include tile scan for stitching large sample areas or mark and find for automatic Z stacks of multiple areas of interest.
Leica SP8X Multiphoton Confocal
This high-speed multiphoton imaging system is equipped with a Coherent Vision S Ti-Sapphire laser for maximum flexibility in stain choices, and high sensitivity GaASp detectors for increased signal to noise ratio. Multiphoton imaging is less phototoxic to live cells since IF wavelengths, as opposed to UV wavelengths, are utilized.
The Cytoviva uses patented illumination technology to collect high signal-to-noise, transmitted darkfield images of scattered light from nanoparticles. The hyperspectral imaging system captures the visible and near infrared spectrum within each pixel of the field of view. This allows for detailed spectral analysis of the scattered light from scanned materials. This "spectral library" for specific nanomaterials can be used to confirm the presence of the nanomaterials in cells, tissue, or composites.
Leica DM750P Polarized Light
The DM750P is an entry-level polarized light microscope. The object guide allows precise X/Y specimen positioning, and the stage brake prevents stage movement. The scope features a strain-free condenser for magnifications from 4X to 100X and 4 strain-free objectives (10X, 40X, 63X, and 100X). The microscope can be coupled to a Leica DFC290 HD 3 MP color camera for image capture.
|Objective||Numerical Apeture||Immersion Media|
|100X||1.25||Hi PLan Oil|
Leica M125 Stereoscope
This ergonomic and modular routine stereoscope features an ESD design, a large field of view, and a 10:1 zoom range. The microscope is equipped with the Leica LED3000 NVITM LED light source and a Leica DFC290HD, 3 mp color camera. Additional light sources are available, including spotlight LEDs and a LED ring light.
Leica Laser Microdissection
The laser microdissection (LMD) system, also known as laser capture microscopy (LCM), allows users to use a non-contact method to isolate homogenous samples from heterogenous starting material. After dissecting regions of interest from the initial tissue sample, or isolating cells from cell culture, purified samples can be analyzed downstream using a variety of analysis methods such as PCR or proteomic studies. The starting material can be arranged on slides or petri dishes, and the collected samples are collected in individual PCR tubes or strips.