This spectral confocal features four laser lines (405 nm, 488 nm, 532 nm, and 635 nm) and tunable emission filters, with scan speeds ranging from 400 to 800 Hz. Brightfield and DIC modes are also available. We offer four ACS APO objectives on this system: 10x/0.3 dry, 20x/0.6 mixed immersion, 40x/1.15 oil, and 63x/1.3 oil. Software features include tile scan for stitching large sample areas or mark and find for choosing multiple areas of interest for imaging. Leica’s 3D viewer software allows users to save images as a volume view, a shadow view, or a maximum intensity projection. Individual snapshots or all images from a Z-stack may also be exported.