The Eclipse TiE is a fully motorized, perfect focus microscope. The system is fully motorized and computer controlled for six-dimensional imaging. The motorized encoded Z drive has three sensitivities with a minimum step size of 25 nm. The Eclipse TiE microscope is equipped with brightfield and DIC, as well as epi-fluorescence and TIRF laser sources. A variety of objective lenses are available for the Eclipse TiE, which may be coupled with a 1.5X intermediate zoom. The Clemson Light Imaging Facility has dry, water immersion and oil immersion objective lenses ranging from 4X to 100X, including the Nikon 60X TIRF oil immersion lens with the world's highest numerical aperture of 1.49.