Bacterial Engineering

Description of the Activity

In this laboratory students will have the opportunity to work with bacterium E. coli. Two new genes will be inserted into this bacterial genome, one for the production of green fluorescent protein and another for resistance to ampillicin. A promotor sequence will also be transferred, allowing the expression of the two new genes to be regulated. After incubation, the transformed culture will be compared to the control to testi for the ability to produce glow in the dark colonies grow in the presence of ampicillin.

Objectives

  • Identify the structure of bacterial cells
  • Describe the bacterial genome
  • Explain the process of genetic transformation
  • List the parameters necessary for successful transformation
  • Discuss how phenotype is a result of genotype
  • Identify the role of selective markers in genetic engineering
  • Understand the process of gene regulation

Meets SC Content Standards