Restriction Analysis of DNA

Description of the Activity

In this experiment students learn all about the structure of DNA and how it can be manipulated by using a variety of techniques. The procedure demonstrates the cutting of DNA from the bacteriophage lambda with restriction enzymes. The DNA fragments are then analyzed by agarose gel electrophoresis.

Objectives

  • Identify the structure and function of DNA
  • Define genetic polymorphism and comprehend how this is exploited for forensic applications
  • Explain the activity of restriction endonucleases
  • Understand the physics of gel electrophoresis
  • Learn techniques for collecting, transferring and loading micro amounts of liquid materials

Meets SC Content Standards