Clemson Translational Research Imaging Core (CTRIC)

Our projects are supported by state-of-the-art core facilities in Clemson, Greenville, and Charleston. CTRIC is lead by core director: Dan Simionescu, a Jerry E. and Harriet Calvert Dempsey Professor, and core co-directors: Terri Bruce, Research Assistant Professor and Clemson Light Imaging Facility Director and Russell Norris, Associate Professor at Regenerative Medicine Department at MUSC.

Project Overview

The Clemson Translational Research Imaging Core (CTRIC) is supported by two thrusts and serves the needs of our COBRE projects by serving as a portal to expertise, training, and mentoring in the following areas: preclinical validation of regenerative medicine approaches, animal models, surgery and bioimaging, utilization of stem cells, molecular biology, histology, and advanced microscopical imaging and biophotonics. The Core provides all the necessary support to develop ideas, overcome technical challenges, and lead projects toward significant outcomes. CTRIC labs, facilities, and expertise are available to all previous and current COBRE scientists and students as well to all SC specialists from academia, medical research institutions, and industry. Services are provided by two individual thrusts.

Focus Areas

Thrust I: Bio-Analytical

  • Stem Cell Biology: Stem cell isolation, Stem cell differentiation, Cell phenotyping
  • Advanced imaging: Light, polarized microscopy, Fluorescent microscopy, Electron microscopy
  • Advanced Histology: Tissue processing, Classical/special histology, Immunostaining
  • Molecular biology: Protein expression, Gene expression, Gene transfection, silencing
  • Digital Image Processing: 3D reconstruction

Thrust II: Pre-Clinical Validation Thrust

  • Consultations: Animal models, Grant proposals, IACUC protocols
  • Animal care: Pre-operative care, Post-operative care
  • In Vivo Imaging: Ultrasound, Doppler, MicroCT, IVIS Lumina XR
  • Technical Services: Surgical assistance, Euthanasia, Blood, sample collection
  • Clinical imaging: Large animal MRI, Large animal CT

Thrust Facilities and Locations Leader and Support Personnel Services

I. Bio-Analytical

Regenerative Medicine and Stem Cell Laboratory at Greenville and Clemson

Dr. Dan Simionescu

Dr. Agnes Nagy-Mehesz

Stem cell biology and differentiation, cell sorting and in vivo tracking

Light Imaging and Electron Microscopy Facility at Clemson and Charleston

Dr. Terri Bruce

Dr. Russell Norris

Confocal, hyperspectral imaging, FACS, TEM, SEM, 3D cell and tissue imaging

II. Pre-Clinical Validation

Godley Snell Research Center and Experimental Farm at Clemson

Dr. John Parrish

Travis Pruett

Development and oversight of IACUC protocols, small animal model studies, bioimaging

Godley Snell Research Center, Experimental Farm at Clemson, AVS Image Analysis Laboratory

Dr. Jeryl Jones

Tina Parker

Development and oversight of IACUC protocols, large animal model studies, clinical bioimaging

View a list of instruments in the core. Additional information about complete instrumentation and services provided through the Clemson Light Imaging Facility (CLIF), Clemson Electron Microscopy, and Godley Snell Research Center

For more information about our core facilities, services, and consultations please contact our core director, Dr. Simionescu, or lab manager Dr. Nagy Mehesz.


CTRIC Core Billing Rates

CTRIC Rates
CTRIC Core Billing Rates, FY 22/23
Description of Commodity or Service Unit of Billing Internal Rates
(Academia)
External Rates
(Corporate)
Cell culture room (sterile room with all utilities for cell culture work) per hour $ 15.00 $ 30.00
Classical histology staining (supplies included)   per slide $ 5.00 $ 10.00
Frozen embedding (supplies included) per hour $ 20.00 $ 40.00
Frozen sectioning (cryotome)(supplies included) per hour $ 20.00 $ 40.00
Flow Cytometry (Attune NxT) per hour $ 50.00 $ 100.00
Fluorescent microscopy, Keyence BZ-X810 per hour $ 15.00 $ 30.00
H&E Staining per hour $ 1.00 $ 3.00
Immunohistochemistry (supplies, but not primary antibody included) per slide $ 7.00 $ 20.00
Paraffin embedding (embedding station) (supplies included) per hour $ 15.00 $ 30.00
Paraffin sectioning (microtome) Supplies included per hour $ 15.00 $ 30.00
qPCR, Reverse Transcription (Rotor gene Q) per hour $ 30.00 $ 60.00
Western Blot, automated (Jess) per one run $ 130.00 $ 300.00
Tissue Processing for paraffin embedding (tissue processor) (supplies included) per one run $ 30.00 $ 60.00
Consultation per hour n/a $ 100.00
Technical Assistance per hour n/a $ 100.00