Clemson Translational Research Imaging Core Instrumentation

Attune NxT Flow Cytometer, Thermo Fisher

Features

• Acoustic-assisted hydrodynamic focusing for high accuracy even at high speed
• Holds tubes from 17x100mm to 8.5x45mm
• High-throughput workflow with an autosampler (standard/deep 96 and 384 well plates)
• Easy-to-use software
• 3 lasers: violet (405nm), blue (488nm), red (637nm)
• 13 parameters: VL1(440/50), VL2(512/25), VL3(603/48), VL4(710/50), FSC, SSC, BL1(488/10), BL2(574/26), BL3(695/40), BL4(780/60), RL1(670/14), RL2(720/30), RL3(780/60)

Applications

• Molecular biology (cell cycle, viability, apoptosis)
• Immunophenotyping
• Stem cell, cancer, plant biology

Jess Automated Western Blot system, Protein Simple

Features

• Automated, capillary-based immunoassay
• Sample volume: 3µl, total processing time: 3-4h
• 13 or 25 samples run simultaneously, independently
• Fluorescent (IR and NIR channel), chemiluminescent, total protein detection modes
• Recently introduced the reprobing capability
• Easy to use operating/analyzing software

Applications

• Molecular biology
• Stem cell, cancer biology

BZ-X810 All-in-one Fluorescent Microscope, Keyence

Features

• Inverted, fully- motorized microscope, built-in darkroom
• High-resolution brightfield, phase-contrast, fluorescent (DAPI, GFP, TRITC, Cy5, Cy7) imaging
• 2x, 10x, 20x, 40x lenses
• Accommodates slides, dishes, well-plates
• Z-stacking, image stitching, cell counting, optical sectioning, time-lapse imaging
• Incubation and live-cell imaging
• Easy to use operating/analyzing software

Applications

• Life-sciences, Immunology, neurology research
• Stem cell, cancer research
• Histology, histopathology

Biological safety cabinet 1300 Series Class II, Type A2, Thermo Scientific

Features

• UV light with safety lockout
• HEPA filters in the supply and exhaust airflow
• Constant downflow avoids cross-contamination
• Digital Airflow Verification with a visual and audible alarm
• Internal outlets and service valves on each side

Applications

Sterile cell culture work

Laboratory CO2 Incubator, Isotemp, Fisher Scientific

Features

• Water-jacketed, 6.5 cu ft capacity chamber
• Temperature setpoint range 10oC – 55oC
• Provide stable temperature, humidity, and CO2 level
• Low and high CO2, power interruption, and temperature deviation alarms
• Microbiological filters protect gas inlet and sample port
• Easy-to-use display

Applications

Sterile cell culture work

5417C Microcentrifuge, Eppendorf

Features

• Fixed angle rotor
• Max. speed: 14000RPM
• Max. capacity: 30 x 1.5 or 2ml tubes
• Timer: 0 to 99 min. in 1min. increment
• Backlit, full-digit display for easy reading

Applications

• Protein isolation
• Nucleic acid isolation

Scepter 2.0 handheld automated cell counter, Millipore

Features

• Cell size range: 6 - 36µm
• Concentration range: 10000 – 500000 cell/ml
• Processing time: 30s
• Screen displays cell concentration, average cell size and volume, a histogram of size or volume distribution
• Stores 72 histograms, USB cable port for data retrieving

Applications

• Cell counting
• Cell health monitoring
• Cell behavior monitoring

Guava Easy Cyte5 Flow Cytometer, Millipore

Features

• A microcapillary flow cytometry system
• Easy to operate, uses small sample volume (50µl)
• Excitation laser: Blue (488nm)
• Emission filters: Red1 (690/50nm), Green (525/30nm), Yellow (583/26nm)
• 2 scatters: Forward and Side

Applications

• Cell counting
• Immunophenotyping
• Cell signaling
• Cell health and toxicity screening
• Cell viability, cell cycle assays

Epoch microplate spectrophotometer, BioTek

Features

• Wavelength scanning, endpoint, kinetic and well area scanning
• Accommodates standard 6-,12-, 24-,48-,96- and 384 well microplates
• Wavelength range 200 to 900nm
• Intuitive and user-friendly Gen5 analysis software
• Special Take3 multi-volume plate for very low volume (2µl) of samples

Applications

• Colorimetric Elisa
• Protein quantification
• Nucleid acid (ssDNA, dsDNA, RNA) quantification

Mini-Protean 3 electrophoresis system, Bio-Rad

Features

• Accepts hand-cast and precasted BioRad gels
• Runs one or two gels simultaneously
• Additional blot module for performing the transfer
• Universal power supply

Applications

• SDS-PAGE protein separation
• Nucleic acid PAGE separation

ChemiDoc It2 Imager, UVP

Features

• Image capture, quantitative analysis of chemiluminescent blots and gels
• Transilluminator on a sliding tray for easy sample placement
• Epi White Lights built into the top for lighting and focusing
• Four-position filter wheel, with included ethidium-bromide one
• LED white light plate for Coomassie blue and silver staining
• VisionWorksLS Image Acquisition and Analysis software

Applications

• Protein gel
• DNA gel
• Chemiluminescent blot

Rotor-Gene Q real-time PCR cycler, Qiagen

Features

• Enables high-precision real-time PCR, endpoint PCR, and high resolution melt analysis
• Unique rotary format (36 or 72 well rotor) for thermal and optical uniformity
• 5 available channels: blue, green, yellow, orange, red, and crimson
• The comprehensive Rotor-Gene Q software supports all current real-time analysis procedures

Applications

• Gene expression analysis
• Genotyping
• Pathogen detection
• Methylation analysis

Vacuum infiltration processor, Tissue-Tek VIP5, Sakura

Features

• Automatic tissue processor holds up to 150 tissue cassettes or up to 300 cassettes
• Holds 15 bottles ( 10 for processing reagents, 3 for cleaning cycle, and 2 for waste)
• VIP software is programmable for up to twenty different programs for use in the fixation, dehydration, clearing, and paraffin infiltration
• Delay mode allows programming the desired end time (the program automatically calculates the start time)

Applications

Suitable for a variety of human, animal, or plant tissue specimens

Paraffin embedding workstation, HistoStar, Thermo Scientific

Features

• Brightly illuminated and clutter-free LEDlighting for uniform illumination of the workplace
• Smooth and insulated contact points
• 5L paraffin capacity, cold plate area for 72 base molds, large heated workspace, and specimen holding area
• Adjustable paraffin dispense paddle
• Heated wax trimmer built into the workspace
• Large, easy-to-read touchscreen display control

Applications

Embedding routine tissue samples into paraffin blocks for microtome sectioning

Automated cryostat, Leica CM1950, Leica Biosystems

Features

• -35oC cryo-chamber with 15 freezing stations
• Motorized sectioning with slow (300µm/s in 20µm steps) or fast (900µm/s) electric coarse feed
• Section thickness range: 1 to 100µm
• Automatic cryo-chamber defrosting
• Control panels comfortably positioned in the armrests
• Built-in UVC disinfection system and AgProtect antimicrobial nanosilver coating on the outside surfaces
• Section Waste Removal system with a series of three filters (including HEPA)

Applications

Sectioning frozen tissues

Automated rotary microtome, Leica RM2255, Leica Biosystems

Features

• Allows motorized as well as manual sectioning
• Motorized sectioning with slow (300µm/s) or fast (900µm/s) electric coarse feed
• Section thickness range: 0.5 to 100µm
• Precise specimen orientation with zero-point reference
• Intuitive control panel
• Spacious and easy-to-clean section waste tray

Applications

Sectioning paraffin and resin embedded specimens

BondMax automated IHC/ISH staining system, Leica Biosystems

Features

• Fully automation ensures the same high-quality staining each time
• Ready to use reagents eliminate preparation work
• Three independent slide trays ensure flexibility and continuous staining
• Stains more slides ( total capacity of 30) in less time offering greater efficiency
• Trays keep slides organized, not random

Applications

• immunohistochemistry (IHC)
• In situ hybridization (ISH)

Vevo 2100 Ultra Sound Imaging system, Fuji Film, Visual Sonic Inc

Features

• Easy to use real-time, dynamic screening
• B-mode (2D) imaging with 30micron resolution, frame rates up to 1000fps
• M-mode for motion assessment, Pulsed-wave Doppler for blood flow quantification, Color Doppler for flow direction, velocity, Power Doppler for relative quantification of flow
• MicroMarker Contrast Imaging for relative perfusion and targeted molecular data
• Warmed platform, integrated physiological monitoring
• Dedicated micro-injection system
• Transducer mounting system

Applications

• Cardiovascular, Cancer, Developmental biology
• Regenerative medicine, Drug development, Contrast Imaging

Vevo IVIS Lumina XR, Perkin Elmer

Features

• Small animal optical (bioluminescent and fluorescent) imaging system and X-ray
• Optical and X-ray overlay
• Up to 21 emission filter sets to be used to capture emission from green to near-infrared
• Superior spectral unmixing
• Heated stage, anesthesia connection, syringe injection system

Applications

• Orthopedics, Infectious diseases, oncology
• Regenerative medicine, Drug delivery, Nanoparticle tracing

SkyScan 1176 MicroCT, Bruker

Features

• 11-megapixel X-ray detector
• Image field width 68mm, length 220mm: full body scan of mouse and rat
• User determined resolution: 9, 18, 35µm
• Integrated physiological monitoring
• Easy to use software with bone morphometry, 2D & 3D quantitative analysis, 3D visualization

Applications

• Bone and soft tissue imaging
• Material sciences

Aquilion 16 CT scanner, Toshiba

Features

• Multi-helical CT scanner
• 60kW generator, 7.5MHU anode grounded tube
• 0.5mmX16 detector row
• Leading low contrast resolution
• Quantum denoising software
• 12frames/s image reconstruction and display
• Dual LCS flat-panel monitors

Applications

• General imaging, trauma, emergency situations
• Basic lung and cardiac studies

Vet-MR Grande, Esaote

Features

• High-quality MRI scanner
• 0.25 Tesla C-shaped, permanent magnet
• Light patented RF shielding
• Complete set of dedicated linear and DPA coils
• DICOM compliant
• Storage PACS workstation

Applications

Ideal for swine, sheep, and other large animals