Genetics and Biochemistry

Dr. Leigh Anne Clark

Leigh Anne Clark

Assistant Professor

Ph.D. Genetics
2004, Texas A&M University

Contact Information
Office: 057B Life Sciences Building
Phone: (864) 656-4696
Email: lclark4@clemson.edu
Web: Clemson Canine Genetics
Facebook: Canine Genetics Research

Research Focus Areas
Canine Genetics

 

Research Activities

My laboratory studies canine inherited diseases to 1) improve the health and quality of life for dogs and 2) use the dog as a model to better understand the genetics underlying mammalian hereditary diseases. Dog breeds are homogenous populations that exhibit unrivaled phenotypic diversity and present unique opportunities for analysis of both simple and complex traits. Specifically, my research concerns the developmental genetics of canine pigmentation patterns. One focus of this work is merle, a semi-dominant coat pattern characterized by patches of full pigment on a dilute background (Figure 1). Dogs homozygous for merle exhibit auditory and ocular anomalies that are phenotypically similar to those of Waardenburg Syndrome type 2 in the human. Another focus is harlequin coat patterning, a bigenic trait resulting from the interaction of the harlequin locus and the merle locus. Harlequin is a dominant modifier of merle that removes the dilute pigment, leaving the background white (Figure 1). Another facet of my research concerns dermatomyositis, an inflammatory disease of the skin and muscle that is found predominantly in Collies and Shetland Sheepdogs. Finally, my laboratory is investigating the genetic basis of two prominent diseases of the German Shepherd Dog: pancreatic acinar atrophy, a disorder that causes exocrine pancreatic insufficiency (Figure 2) and megaesophagus, a congenital dysfunction of the esophagus that causes neonatal mortality. To dissect the genetic bases for these disorders my laboratory employs various tools to carry out genome-wide association studies. A major goal of our research is to develop commercially available tests to allow for early detection of disease and enable breeders to eliminate affected and carrier dogs from breeding programs.

Great Dane coat patterns
Figure 1. Great Dane puppies with three different coat patterns (from left to right): merle, harlequin, and solid. Solid-coated puppies may or may not carry harlequin.

German Shepard with Exocrine Pancreas Insufficiency
Figure 2. A German Shepherd Dog with exocrine pancreatic insufficiency.

 

Grant Support

National Institutes of Health
Naturally Occurring Dog Model for Juvenile Dermatomyositis

Collie Health Foundation
Collie Whole-genome Sequence and Genome-wide Association Studies

Great Dane Club of America Charitable Trust
Great Dane Whole-genome Sequence Assembly

 

Awards

Clemson University Research Grant Committee Award
 

Research Associates

 

Graduate Students

 

Undergraduates

Brittany Rosener
Mark Neese

 

Courses Taught

GEN 4500/6500
Comparative Genetics

GEN 4700/6700
Human Genetics

Resources

 

Recent Publications

Rinz, C.J., J. Levine, K.M. Minor, H.D. Humphries, R. Lara, A.N. Starr-Moss, L.T. Guo, G.D. Shelton, and L.A. Clark (2014) A COLQ Missense Mutation in Labrador Retrievers Having Congenital Myasthenic Syndrome. PLoS ONE 9(8): e106425.

Clark, L.A. and A.N. Starr-Moss (2013). Genetics and Genomics of the Domestic Dog. In: Animal Molecular and Quantitative Genetics. Ed: H. Khatib. Hoboken, New Jersey. Wiley-Blackwell. (in press)

Tsai, K.L., A.N. Starr-Moss, G.M. Venkataraman, C. Robinson, L.j. Kennedy, J.M. Steiner, and L.A. Clark (2013). Alleles of the major histocompatibility complex play a role in the pathogenesis of pancreatic acinar atrophy in dogs. Immunogenetics 65: 501-509.

Noorai, R.E., N.H. Freese, L.M. Wright, S.C. Chapman* and L.A. Clark* (2012). Genome-wide Association Mapping and Identification of Candidate Genes for Rumpless and Ear-tufted Traits of the Araucana Chicken. PLoS ONE 7(7):e40974.

Clark, L.A. and M.L. Cox (2012). Current status of genetic studies of exocrine pancreatic insufficiency in dogs. Topics in Companion Animal Medicine 10.1053/j.tcam.2012.04.001.  

Tsai, K.L., R.E. Noorai, A.N. Starr-Moss, P. Quignon, C.J. Rinz, E.A. Ostrander, J.M. Steiner, K.E. Murphy and L.A. Clark (2012). Genome-wide association studies for multiple diseases of the German Shepherd Dog. Mammalian Genome 23:203-211. 

Gill, J.L., K.L. Tsai, C. Krey, R.E. Noorai, J.F. Vanbellinghen, L.S. Garosl, G.D. Shelton, L.A. Clark* and R.J. Harvey* (2012). A canine BCAN microdeletion associated with episodic falling syndrome. Neurobiology of Disease 45 (1):130-136.

Clark, L.A., K.L. Tsai, A.N. Starr, K.L. Nowend and K.E. Murphy (2011). A missense mutation in the 20S proteasome B2 subunit of Great Danes having harlequin coat patterning. Genomics 97: 244-248.

Spadafora, D., E.C. Hawkins, K.E. Murphy, L.A. Clark and S. T. Ballard (2010). Naturally-occurring mutations in the canine CFTR gene. Physiological Genomics 42: 480-485.

Strain, G, L.A. Clark, J.M. Wahl, A. Turner and K.E. Murphy (2009). Prevalence of deafness in dogs heterozygous or homozygous for the merle allele. Journal of Veterinary Internal Medicine 23: 282-286.