Post Doc, Massachusetts Institute of Technology, 1983
Research Interests: Biotechnical Polymers, Stimulated Release, Enzyme Engineering
Prof. Guiseppi-Elie's research in chemical and biomolecular engineering focuses on...
Biotechnical Polymers: The synthesis, characterization and pre-clinical application of bioactive monomers for inclusion into a broad range of bioactive and biosmart (biotechnical) polymers for biomedical applications. This is illustrated by the demonstrated development of an all synthetic pancreas wherein the polymer alters its rate of release of insulin (a polypeptide) in response to changing levels of glucose (a simple sugar) (see Guiseppi-Elie et al. Advanced Materials (2002) 14(10): 743-746.). Of interest are: i) The use of biological molecules that confer molecular recognition, such as peptide sequences, RNA fragments and enzymes for integration with synthetic polymers to make bioactive membranes of biosensors. ii) The use of bio-derived or bio-inspired monomer that confer biocompatibility through mitigation of the foreign body response associated with implantable devices.
Bioactive Controlled Release: The synthesis and characterization of chemically responsive smart polymeric materials and the design and pre-clinical deployment of smart structures for the programmed release of bioactive agents such as anti-inflammatory drugs and potentiating small molecules and ions. Such polymers respond to externally derived electrical, chemical, magnetic or optical stimuli with the release of bioactive agents. This is illustrated by the demonstrated development of electroconductive hydrogels for the programmed release of Ca++ ions to stimulate cellular activity (See Guiseppi-Elie, Biomaterials (2010) on line and Justin and Guiseppi-Elie, Biomacromolecules (2009) 10(9):2539-2549.). Of interest are: i) The integration of one-dimensional organic conductors (conductive polymers) with hydrogels to make bioactive interfaces of neural prostheses. ii) The integration of carbon nanotubes and metallic nanoparticles with hydrogels to make bioactive interfaces of implantable devices.
Enzyme Engineering: The purposeful alteration of protein structure to effect new and desirable properties in such applications as bioacatalytic transformations in the pharmaceutical and fine chemical industries, bioanalytical devices such as biosensors and in diagnostic microfluidic devices such as lateral flow devices and bioMEMS devices. This is illustrated by the demonstrated formation of enzyme-carbon nanotube (CNT) conjugates for Type 3 biosensors with direct electron transfer between cofactor and electrode (see Guiseppi-Elie et al. NanoBiotechnology (2009), 4, 9-17 and Guiseppi-Elie et al. Nanotechnology (2002) 13 (5) 559-564). Of interest are: i) Site directed mutagenesis for oxidoreductase enzymes for stabilization via PEGylation. ii) The physico-chemical characterization of enzyme-nano materials interactions for cytotoxicity. iii) Enzyme adsorption to nano-structured electrodes.
A. Nolan Wilson and Anthony Guiseppi-Elie “Bioresponsive Hydrogels” Advanced Healthcare Materials (Published on-line December 10th, 2012) doi:10.1002/adhm.201200332
Research premiered on Germany’s nation-wide Deutschlandradio program ‘Science in Focus’ (Sunday, October 28th, 2012). http://www.dradio.de/dlf/sendungen/wib/1905639/
Research featured on NPR/South Carolina (ETV Radio Network) newscast ‘Your Day’ Interviewed by Dr. Peter Kent. (Wednesday, November 21st, 2012). http://yourday.clemson.edu/?q=node/1035
Anthony Guiseppi-Elie, Christian N. Kotanen, Olukayode Karunwei and A. Nolan Wilson “Engineering the Bio-Abio Interface to Enable Next Gen Bionics" Special Issue on Functional Diversification of Semiconductor Electronics” ECS Transactions (2012) (accepted) http://ma.ecsdl.org/content/MA2012-02/36/2785.short.
Christian N. Kotanen and Anthony Guiseppi-Elie “Bioactive Electroconductive Hydrogels Yield Novel Biotransducers for Glucose” Macromolecular Symposia (2012) 317-318(1): 187-197. Issue doi:10.1002/masy.201100164
Anthony Guiseppi-Elie, Chenbo Dong and Cerasela Zoica Dinu “Crosslink Density of a Biomimetic Poly(HEMA)-based Hydrogel Influences Growth and Proliferation of Attachment Dependent RMS 13 Cells” Journal of Materials Chemistry: Themed Issue on Materials for Biosurfaces (invited) J. Mater. Chem. (2012) 22 (37), 19529 – 19539 doi: 10.1039/C2JM32516K (IF 5.10)
Sandro Carrara, Sara Seyedeh Ghoreishizadeh, Jacopo Olivo, Irene Taurino, Camilla Baj-Rossi, Andrea Cavallini, Maaike Op de Beeck, Wayne P. Burleson, Francis Gabriel Moussy, Anthony Guiseppi-Elie, Giovanni De Micheli “Fully Integrated Biochip Platforms for Advanced Healthcare” Sensors: Biochips (2012) 12(8), 11013-11060 (IF 1.92) doi: 10.3390/s120811013
A. Nolan Wilson, Ruth Salas and Anthony Guiseppi-Elie “Bioactive Hydrogels Demonstrate Mediated Release of a Chromophore by Chymotrypsin” Journal of Controlled Release (2012) 150(1) 41-47. doi: 10.1016/j.jconrel.2012.02.026 (IF 6.49)
Christian N. Kotanen, Francis Gabriel Moussy, Sandro Carrara and Anthony Guiseppi-Elie “Implantable Enzyme Amperometric Biosensors” Biosensors and Bioelectronics (2012) 35(1) 14-26. doi: 10.1016/j.bios.2012.03.016 (IF 5.10)
Christian N. Kotanen, A. Nolan Wilson, Ann M. Wilson, Kazuhiko Ishihara and Anthony Guiseppi-Elie “Biomimetic Hydrogels Gate Transport of Calcium Ions Across Cell Culture Inserts” Biomedical Microdevices (2012) 14(3) 549-558. doi: 10.1007/s10544-012-9632-0 (IF 3.39)
Christian N. Kotanen, Chaker Tlili and Anthony Guiseppi-Elie “Bioactive Electroconductive Hydrogels: The effects of electropolymerization charge density on the storage stability of an enzyme based biosensor” Journal of Applied Biochemistry and Biotechnology (2012) 166(4) 878–888. doi: 10.1007/s12010-011-9477-7 (IF 3.17)
Sarah M. Strycharz-Glaven, Rachel M. Snider, Anthony Guiseppi-Elie and Leonard M. Tender “On electrical conductivity of microbial nanowires and biofilms” Energy and Environmental Science (2011) 4 4366-4379. doi:10.1039/C1EE01753E (IF 9.4)
Liju Yang, Adilah Guiseppi-Wilson, Anthony Guiseppi-Elie “Design Considerations in the Use of Interdigitated Microsensor Electrode Arrays (IMEs) for Impedimetric Characterization of Biomimetic Hydrogels” Biomedical Microdevices (2011) 13(2):279-89. doi:10.1007/s10544-010-9492-4 (IF: 2.92)
Anthony Guiseppi-Elie “An Implantable Biochip to Influence Patient Outcomes Following Trauma-induced Hemorrhage” Journal Analytical and Bioanalytical Chemistry (2011) 399(1), 403-419. doi:10.1007/s00216-010-4271-x (IF: 3.84)
Anthony Guiseppi-Elie, Lauren Koch, Stephen H. Finley and Gary E. Wnek “The Effect of Temperature on the Impedimetric Response of Bioreceptor Hosting Hydrogels” Biosensors and Bioelectronics (2011) 26(5) 2275–2280. doi:10.1016/j.bios.2010.09.050 (IF: 5.10)
Christian Kotanen and Anthony Guiseppi-Elie “Development of an implantable biosensor system for physiological status monitoring during long duration space flights” Gravitational and Space Biology (2010), 23(2) 55-63.
Gusphyl Justin and Anthony Guiseppi-Elie*, "An Electroconductive Blend of p(HEMA-co-PEGMA-co-HMMA-co-SPMA) Hydrogels and p(Py-co-PyBA): In Vitro Biocompatibility" Journal of Bioactive and Compatible Polymers (2010), 25(2) 121-140. doi:10.1177/0883911509350660) (IF=0.93)
Anthony Guiseppi-Elie “Electroconductive Hydrogels: Synthesis, Characterization and Biomedical Applications” Biomaterials, (2010) 31(10) 2701-2716. doi:10.1016/j.biomaterials.2009.12.052 (IF: 3.8)
Anthony Guiseppi-Elie, Abdur Rub Abdur Rahman and Nikhil K. Shukla “SAM-modified Microdisc Electrode Arrays (MDEAs) With Functionalized Carbon Nanotubes” Elctrochimica Acta (2010) 55(14), 4247-4255 doi:10.1016/j.electacta.2008.12.043 (IF=2.85)