Anthony Guiseppi-Elie, Sc.D.

Education

Post Doc, Massachusetts Institute of Technology, 1983
Sc.D., Massachusetts Institute of Technology, 1983
M.Sc., University of Manchester Institute of Science and Technology, 1980
B.Sc., (First Class) University of the West Indies, 1979

Research Interests: Biotechnical Polymers, Stimulated Release, Enzyme Engineering

Prof. Guiseppi-Elie came to Clemson University in January 2006 after being a professor of Emergency Medicine (SoM) and Prof. of Chemical and Life Sciences Engineering (SoE) at Virginia Commonwealth University. Prof. Guiseppi-Elie serves as Director of the Center for Bioelectronics Biosensors and Biochips, a plura-disciplinary faculty cluster represented by BioE, ChBE, Chemistry, ECE, and Mat. Sci. of the College of Engineering and Science.

Prof. Guiseppi-Elie's research in chemical and biomolecular engineering focuses on...

Biotechnical Polymers: The synthesis, characterization and pre-clinical application of bioactive monomers for inclusion into a broad range of bioactive and biosmart (biotechnical) polymers for biomedical applications. This is illustrated by the demonstrated development of an all synthetic pancreas wherein the polymer alters its rate of release of insulin (a polypeptide) in response to changing levels of glucose (a simple sugar) (see Guiseppi-Elie et al. Advanced Materials (2002) 14(10): 743-746.). Of interest are: i) The use of biological molecules that confer molecular recognition, such as peptide sequences, RNA fragments and enzymes for integration with synthetic polymers to make bioactive membranes of biosensors. ii) The use of bio-derived or bio-inspired monomer that confer biocompatibility through mitigation of the foreign body response associated with implantable devices.

Bioactive Controlled Release: The synthesis and characterization of chemically responsive smart polymeric materials and the design and pre-clinical deployment of smart structures for the programmed release of bioactive agents such as anti-inflammatory drugs and potentiating small molecules and ions. Such polymers respond to externally derived electrical, chemical, magnetic or optical stimuli with the release of bioactive agents. This is illustrated by the demonstrated development of electroconductive hydrogels for the programmed release of Ca⁺⁺ ions to stimulate cellular activity (See Guiseppi-Elie, Biomaterials (2010) on line and Justin and Guiseppi-Elie, Biomacromolecules (2009) 10(9):2539-2549.). Of interest are: i) The integration of one-dimensional organic conductors (conductive polymers) with hydrogels to make bioactive interfaces of neural prostheses. ii) The integration of carbon nanotubes and metallic nanoparticles with hydrogels to make bioactive interfaces of implantable devices.

Enzyme Engineering: The purposeful alteration of protein structure to effect new and desirable properties in such applications as bioacatalytic transformations in the pharmaceutical and fine chemical industries, bioanalytical devices such as biosensors and in diagnostic microfluidic devices such as lateral flow devices and bioMEMS devices. This is illustrated by the demonstrated formation of enzyme-carbon nanotube (CNT) conjugates for Type 3 biosensors with direct electron transfer between cofactor and electrode (see Guiseppi-Elie et al. NanoBiotechnology (2009), 4, 9-17 and Guiseppi-Elie et al. Nanotechnology (2002) 13 (5) 559-564). Of interest are: i) Site directed mutagenesis for oxidoreductase enzymes for stabilization via PEGylation. ii) The physico-chemical characterization of enzyme-nano materials interactions for cytotoxicity. iii) Enzyme adsorption to nano-structured electrodes.

Selected Publications

Sarah M. Strycharz-Glaven, Rachel M. Snider, Anthony Guiseppi-Elie and Leonard M. Tender, ”On electrical conductivity of microbial nanowires and biofilms” Journal of Environmental Science and Technology (2011) (accepted)

Anthony Guiseppi-Elie, Christian Kotanen and A. Nolan Wilson “Frontiers of More than Moore in Bioelectronics and the Required Metrology Needs” In D.G. Seiler, A.C. Diebold, R. McDonald, A. Chabli, and E.M. Secula, Frontiers of Characterization and Metrology for Nanoelectronics: 2010, American Institute of Physics, Melville, NY, Vol. XXXX, 398 pp. (30 September 2010)

Liju Yang, Adilah Guiseppi-Wilson, Anthony Guiseppi-Elie “Design Considerations in the Use of Interdigitated Microsensor Electrode Arrays (IMEs) for Impedimetric Characterization of Biomimetic Hydrogels” Biomedical Microdevices (2011) 13(2):279-89.  doi:10.1007/s10544-010-9492-4 (IF: 2.92)

Anthony Guiseppi-Elie “An Implantable Biochip to Influence Patient Outcomes Following Trauma-induced Hemorrhage” Journal Analytical and Bioanalytical Chemistry (2011) 399(1), 403-419.   doi:10.1007/s00216-010-4271-x (IF: 3.84)

Anthony Guiseppi-Elie, Lauren Koch*, Stephen H. Finley+ and Gary E. Wnek “The Effect of Temperature on the Impedimetric Response of Bioreceptor Hosting Hydrogels” Biosensors and Bioelectronics (2011) 26(5) 2275–2280. doi:10.1016/j.bios.2010.09.050 (IF: 5.10) (*Governor’s High School Student) (+Clemson Undergraduate)

Christian Kotanen and Anthony Guiseppi-Elie “Development of an implantable biosensor system for physiological status monitoring during long duration space flights” Gravitational and Space Biology (2010), 23(2) 55-63

Gusphyl Justin and Anthony Guiseppi-Elie*, "An Electroconductive Blend of p(HEMA-co-PEGMA-co-HMMA-co-SPMA) Hydrogels and p(Py-co-PyBA): In Vitro Biocompatibility" Journal of Bioactive and Compatible Polymers (2010), 25(2) 121-140. doi:10.1177/0883911509350660) (IF=0.93) 

Anthony Guiseppi-Elie “Electroconductive Hydrogels: Synthesis, Characterization and Biomedical Applications” Biomaterials, (2010) 31(10) 2701-2716.  doi:10.1016/j.biomaterials.2009.12.052 (IF: 3.8)

Anthony Guiseppi-Elie, Abdur Rub Abdur Rahman and Nikhil K. Shukla “SAM-modified Microdisc Electrode Arrays (MDEAs) With Functionalized Carbon Nanotubes” Elctrochimica Acta (2010) 55(14), 4247-4255   doi:10.1016/j.electacta.2008.12.043  (IF=2.85)

Abdur Rub Abdur Rahman, Gusphyl Justin, Adilah Guiseppi-Wilson and Anthony Guiseppi-Elie* “Fabrication and Packaging of a Dual Sensing Electrochemical Biotransducer for Glucose and Lactate Useful in Intramuscular Physiologic Status Monitoring” IEEE Sensors Journal (2009) 9(12): 1856-1863  doi: 10.1109/JSEN.2009.2031347 (IF=1.17)

Gusphyl Justin and Anthony Guiseppi-Elie*, "Characterization of Electroconductive Blends of p(HEMA-co-PEGMA-co-HMMA-co-SPMA) Hydrogels and p(Py-co-PyBA)" Biomacromolecules (2009) 10(9):2539-2549. doi:10.1021/bm900486d (IF=4.15)

Ali Ozgur Boztas and Anthony Guiseppi-Elie* “Immobilization and Release of the Redox Mediator Ferrocene Monocarboxylic Acid from within Cross-linked p(HEMA-co-PEGMA-co-HMMA) Hydrogels” Biomacromolecules (2009) 10(8):2135-2143. doi:10.1021/bm900299b (IF=4.15)

Y. Zhou, B. Yu, A. Guiseppi-Elie, V. Sergeyev and K. Levon* “Potentiometric Monitoring of DNA Hybridization” Biosensors and Bioelectronics (2009) 24, 3275-3280.  (IF=4.13) doi:10.1016/j.bios.2009.04.023

Abdur Rub Abdur Rahman, Gusphyl Justin and Anthony Guiseppi-Elie* “Bioactive Hydrogel Layers on Microdisc Electrode Arrays: Impedance Measurements and Equivalent Circuit Modeling” Electroanalysis (2009) 21(10), 1135-1144. doi:10.1002/elan.200804540  (IF=3.08)

Gusphyl Justin, Abdur Rub Abdur Rahman and Anthony Guiseppi-Elie* “Bioactive Hydrogel Layers on Microdisc Electrode Arrays: Cyclic Voltammetry Experiments and Simulations” Electroanalysis (2009) 21(10), 1125-1134. doi:10.1002/elan.200804548 (IF=3.08)

Anthony Guiseppi-Elie, Sung-Ho Choi and Kurt E. Geckeler “Ultrasonic Processing of Enzymes: Effect on Enzymatic Activity of Glucose Oxidase” Journal of Molecular Catalysis B: Enzymatic (2009) 58, 118–123.  http://dx.doi.org/10.1016/j.molcatb.2008.12.005  (IF=2.01)

Abdur Rub Abdur Rahman and Anthony Guiseppi-Elie “Design Considerations in the Development and Application of Microdisc Electrode Arrays (MDEAs) for Implantable Biosensors” Biomedical Microdevices: BioMEMS and Biomedical NanoTechnology (2009) 11:701-710. http://www.doi.org/10.1007/s10544-008-9283-3 (IF=2.92)

Gusphyl Justin, Stephen Finley, Abdur Rub Abdur Rahman, and Anthony Guiseppi-Elie “Biomimetic Hydrogels for Biosensor Implant Biocompatibility: Electrochemical Characterization using Micro-Disc Electrode Arrays (MDEAs)” Biomedical Microdevices: BioMEMS and Biomedical NanoTechnology (2009) 11:1, 103. http://dx.doi.org/10.1007/s10544-008-9214-3 (IF=2.92)

Abdur Rub Abdur Rahman, Gusphyl Justin and Anthony Guiseppi-Elie “Towards an Implantable Biochip for Glucose and Lactate Monitoring using Micro-Disc Electrode Arrays (MDEAs)” Biomedical Microdevices: BioMEMS and Biomedical NanoTechnology (2009) 11:1, 75. http://dx.doi.org/10.1007/s10544-008-9211-6 (IF=2.92)