Dr. Feng Chen currently has a food chemistry/Phytochemistry lab in 2000 square feet (about 200 M2), which is located in the 2nd floor of the Poole Agricultural Center (P&A) Building in the main campus of Clemson University, Clemson, SC. This lab is equipped with some modern, highly sensitive analytical instruments for chemical analysis. The following equipments are available for this proposed research:
Agilent LC-MS (1200 LC-6110 Quadrupole MS), which is routinely used for analyses of small molecules such as pharmaceuticals, pesticides, nutraceuticlas, etc.
5 Shimadzu’s HPLC systems including: UFLC-UV, 2 HPLC-PDA, HPLC-ELSD, HPLC-fluorescence systems. These HPLC instruments are used to perform essential separation, and identification, as well as quantitative and qualitative calculation of various non-volatile chemicals, including phytochemicals, pesticides, and pharmaceuticals;
Shimadzu’s 17A-QP5050 GC-MS with NIST, Wiley MS database and operating system. It is used for separation, identification, and quantitative analysis of volatile chemicals;
Shimadzu’s GC17A-FID-sniffing port: this instrument permits the identification of the most important or aroma-impact volatile chemicals via AEDA (aroma extraction and dilution analysis) method;
Open-columns in various sizes of length from 30-100 cm, and diameter from 1-10 cm packed with different resins in properties of ion-exchange, size exclusion, polar and non-polar for chemical separation.
Benchmark Genesys UV spectrophotometers and 2 BioTek multiplate readers with UV, fluorescence and chemiluminescence dectrors
*Above chromatographic instruments are all computer connected and remote controlled.
In addition, Dr Chen has an independent cell culture lab (500 square feet) that has a new Labconco biosafety hood, CO2 incubators, PCR, gel electrophoresis, Buchi and Yamoto vacuum evaporators, invert microscopes, Fotodyne transilluminator system, temperate-controlled high speed centrifuges, and auto-collectors, -80C freezer, Metrohm 735 Racimat for OSI index, fermentor, etc., for enzymology, genetic, tissue culture and other food chemistry, phytochemical, and medicinal researches that are funded by NIH, DoD-Natick and other agencies. These projects focus on searching bioactive components from food, medicinal plants, and other agricultural matrix with potent antioxidant, antimicrobial and anticancer bioactivities.