PhD Chemistry and Biochemistry
The University of Southern Mississippi 2009
BS Life Sciences
Fudan University (Shanghai, China) 2002
Toxoplasma gondii widely infects human population. Approximately, one-third of the human population is infected with Toxoplasma parasites. As an obligate intracellular parasite, Toxoplasma has to invade host cells, replicate, and egress to infect another host cell. My lab focuses on two questions of Toxoplasma infection.
1) How Toxoplasma uses its proteases to disseminate infection?
During the lytic cycle of infection, the parasites secrete proteases to maturate their invasion and egress effectors for their proper functions. One of my publications documented that Toxoplasma regulates the activity of one surface-anchored subtilisin-like protease (TgSUB1) by secreting a polypeptide (Saouros, Dou, Marchant, Carruthers, & Matthews, 2012). Toxoplasma also stores its proteases within the Vacuolar Compartment (VAC), a lysosome-equivalent structure, to catabolize ingested proteins for supporting its intracellular replication (Dou, McGovern, Di Cristina, & Carruthers, 2014). The VAC also can help parasite eat “itself” via an autophagy process to maintain its chronic infection (Di Cristina et al., 2017).
2) How Toxoplasma acquires and utilizes nutrients from host cells?
During its intracellular infection stage, Toxoplasma is encapsulated in a membrane-bound niche, termed the parasitophorous vacuole (PV). The membrane of the PV (PVM) limits diffusion of host substances. Moreover, the PV is non-fusogenic, blocking the parasite’s access to ample nutrients generated by the host lysosome. To help acquire host nutrients, Toxoplasma secretes proteins from the unique organelle, the dense granule, that decorate the PV. The putative nutrient pores are believed to be distributed on the PVM, which allow small substances whose molecular weights are less than 1,200 Da to cross. Toxoplasma also can utilize a unique structure within the PV, termed intravacuolar membrane network (IVN), to acquire host macromolecular substances via endocytosis (Dou et al., 2014).
Using a combination of molecular biology, biochemistry, and cell biological approaches, my laboratory will study these two questions at molecular and cellular levels. Our work will shed light on the development of novel strategies to specifically block the proteolytic activity and the nutrient acquisition within the parasites to benefit clinical management of infection.
Di Cristina, M., Dou, Z., Lunghi, M., Kannan, G., Huynh, M.H., McGovern, O., Schultz, T., Schultz, A., Miller, A., Hayes, B., van der Linden, W., Emiliani, C., Bogyo, M., Besteiro, S., Coppens, I., Carruthers, V.B., 2017, “Toxoplasma depends on lysosomal consumption of autophagosomes for persistent infection”, Nature Microbiology, 2, 17096
Cai, F., Dou, Z., Bernstein, S.L., Leverenz, R., Williams, E.B., Heinhorst, S., Shively, J.M., Cannon, G.C., and Kerfeld, C.A., 2014, “Advances in understanding carboxysome assembly in Prochlorococcus and Synechococcus implicate CsoS2 as a critical component”, Life, 5(2): 1141-71
Dou, Z., McGovern, O., Di Cristina, M., and Carruthers, V.B., 2014, “Toxoplasma gondii ingests and digests host cytosolic proteins”, mBio, 5(4): e01188-14 (Highlighted by a spotlight article in Trends in Parasitology)
Warring, S., Dou, Z., Carruthers, V.B., McFadden, G., and van Dooren, G., 2014, “Characterization of the Chloroquine Resistance Transporter homologue in Toxoplasma gondii”, Eukaryotic Cell, 13(11): 1360-70 (cover article)
Liu, J., Pace, D., Dou, Z., Guidot, D., Carruthers, V.B., and Moreno, N.J., 2014, “A Vacuolar-H+-Pyrophosphatase (TgVP1) is Required for Microneme Secretion, Host Cell Invasion, and Extracellular Survival of Toxoplasma gondii”, Mol Microbiol, 93(4):698-712
Dou, Z., Carruthers, V.B., 2013, TgCPL peptidase. For Handbook of Proteolytic Enzymes, Third Edition. Elsevier Academic Press
Dou, , Coppens, I., and Carruthers, V.B., 2013, “Non-canonical maturation of two papain-family proteases in Toxoplasma gondii”, J Biol Chem, 288:3523-34, (cover article)
Saouros, S.*, Dou, Z.*, Henry, M., Simpson, P., Carruthers, V.B., and Matthews, S., 2012, “Inhibition of SUB1 from Toxoplasma gondii by MIC5 via a mechanism similar to subtilisin prodomains”, J Biol Chem, 287:36029-40, (* co-first authors)
Dou, and Carruthers, V.B., 2011, “Cathepsin proteases in Toxoplasma gondii”, Cysteine Proteases of Pathogenic Organisms, Adv Exp Med Biol, 712:49-61.
Iancu, C.V., Morris, D.M., Dou, Z., Heinhorst, S., Cannon, G.C., and Jensen, G.J., 2010, “Organization, structure, and assembly of α-carboxysomes determined by electron cryotomography of intact cells”, J Mol Biol, 396:105-17
Menon, B.B., Dou, Z., Heinhorst, S., Shively, J.M., and Cannon, G.C., 2008, “Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species”, PLoS One, 3, e3570
Dou, Z., Heinhorst, S., Williams, E.B., Murin, C.D., Shively, J.M. and Cannon, G.C., 2008, “CO2 Fixation kinetics of Halothiobacillus neapolitanus mutant carboxysomes lacking carbonic anhydrase suggest the shell acts as a diffusion barrier for CO2”, J Biol Chem, 283:10377-84