Analysis

Molecular characterization and quantification of metabolites and other chemical compounds is a vital technique to all disciplines whose emphasis ranges from genes to ecosystems. Along with the routine quantitation and characterization of compounds that are of natural and synthetic origins, MUAL undertakes method-development for improved and more sensitive quantitation of compounds through various sample purification/concentration and analytical approaches. We work closely with PIs and students from the setup of experiments to data analysis to fulfill all their analytical needs.

Some of the routine analysis we have and can perform are listed below. We are willing to work with researchers to optimize  other procedures for which we have the equipment capacity.  Please contact us to determine if we have the capability to conduct analytical techniques that may not be listed below.

Plant analysis

Metabolite profiling
Capable of identifying and quantifying >1000 metabolites from plant tissue matrices

Glucosinolate profiling
Detailed analysis of the glucosinolates and their respective isothiocyanates

Polyphenol profiling
Quantification and structural elucidation of simple and complex plant polyphenols

Root exudate characterization
Growth optimization for exudate harvest and detailed screening of secondary metabolites

Soil Analysis

Residue analysis of organic compounds

Degradation kinetics of organic compounds

Sorption desorption kinetics of organic compounds

Soil nutrient pools and dynamics
Nitrate, ammonia, organic nitrogen and phosphate dynamics

Potential ammonification, potential nitrification

Microbial enzyme analysis
Activity and dynamics of various C, N and P mineralizing extracellular enzymes

Amino acid profiling

Protein quantitation


Microbial identification & community analysis
(in pure-culture, soil and decomposing litter)

Fatty acid analysis (FAMEs)

Phospholipid fatty acids (PLFA)

Physiological profiling

Fungal: bacterial biomass
Based on ergosterol, muramic acid, glucosamine ratios