Neeraj V. Gohad, Ph.D.

Assistant Research Professor

Contact Information

319 Jordan Hall
Phone: 864-656-3597
Fax: 864-656-0435
Email: neerajg@clemson.edu


Education 

  • BS, Microbiology, University of Pune, 2003
  • PhD, Biological Sciences, Clemson University, 2008

Research Interests

Systems Biology: Organisms-Cells-Molecules

  • Using a variety of invertebrate model systems my research focuses on understanding the evolution and function of G-Protein Coupled Receptors (GPCR) in development and sensory perception. Utilizing biophysical methods like 2-Photon Microscopy and Non-linear optics combined with genomic and proteomic approaches, my goal is to; Understand commonalities in spatial distribution and function of sensory GPCRs across phylogeny. Study the convergent evolution and/or evolutionary conservation of receptor classes and signal transduction pathways and to elucidate the crucial role GPCRs play during development and metamorphosis. I am keenly interested in holometabolic metamorphic induction from mechanistic, functional (imaging) as well as genetic standpoints (proteomics and transcriptomics).
Images by Neeraj Gohad

Adhesion: Interactions between Biolgogical Systems and Engineered Polymer Surfaces

  • Utilizing a cross-disciplinary approach I am working to understand the interactions between engineered polymer substrates and biological systems at several scales (appendages—cells—receptors). A wealth of fundamental and translational information can be garnered by characterizing this interaction at the molecular level. Particularly, I am interested in understanding the effect of bioactive polymers on transducers such as G-Protein Receptor Kinases (GRKs) and downstream effectors like cytoskeletal elements through receptor stimulation. The goal of this work is to understand the physical as well as biological mechanisms behind these interactions and apply this knowledge to the development of novel biomaterials for maritime and biomedical applications.

Images by Dr. Neeraj Gohad

  • I am also interested in understanding adhesion from a physicochemical standpoint, especially in marine larval bio-adhesives. I am currently working to understanding the chemical nature and synthesis of these adhesives along with the biology of adhesive production. As these bio-adhesives are found in extremely small amounts in nature, conventional analysis techniques are unusable; to this end I am employing quantitative biophysical techniques such as 2-Photon Microscopy combined with and through collaborative efforts, chemical imaging techniques like Coherent Anti-Stokes Raman Scattering Spectroscopy to better understand chemical composition of these adhesives. Understanding under water adhesion has the potential to inspire design of synthetic adhesive systems which have a variety of applications in medicine and surgery.

Images by Dr. Neeraj Gohad

Instrumentation Development

  • Recent advances in optical microscopy have transformed neuroscience, cell biology, and biomedicine. I am working to construct and adapt instrumentation for biophysical techniques like 2-Photon Microscopy and CARS for studying developmental and metabolic processes in invertebrate model systems.

Images by Dr. Neeraj Gohad


Selected Publications

  1. Aldred, N., N. V. Gohad, L. Petrone, B. Orihuela, B. Liedberg, T. Ederth, A. Mount, D. Rittschof and A. S. Clare. 2013. Confocal microscopy-based goniometry of barnacle cyprid permanent adhesive. The Journal of Experimental Biology 216(11): 1969-1972.  (This article was on the Cover of Journal of Experimental Biology)
  2. NV Gohad, N Aldred, B Orihueladiaz, A Clare, D Rittschof, AS. Mount. 2012. Observations on the settlement and cementation of barnacle (Balanus amphitrite) cyprid larvae after artificial exposure to noradrenaline and the locations of adrenergic-like receptors. Journal of Experimental Marine Biology and Ecology Volume 416-417 (2012) 153–161
  3. Philip M. Imbesi, NV Gohad*, Michael J. Eller, Beatriz Orihuela, Dan Rittschof, Emile A. Schweikert, Andrew S. Mount, and Karen L Wooley. 2012. Noradrenaline-Functionalized Hyperbranched Fluoropolymer-Poly(ethylene glycol) Cross-Linked Networks As Dual-Mode, Anti-Biofouling Coatings. ACS-Nano VOL. 6-NO. 2, 1503-1512 (* equal contributor author)
  4. N Pradhan, NV Gohad*, T Burg, S Birchfield, AS  Mount. 2011. Autonomous Tracking and Analysis of Barnacle Cyprid Exploratory Behavior. Journal of experimental marine biology and ecology: Volume 410, 15 December 2011, Pages 21-28 (*equal contributor author)
  5. AS Mount, NV Gohad*, AT Metters, NM Shah. 2011 Method and compositions for Biofouling Deterrence (2009), United States patent application, Pub. No.: US 201110123477 Al (*equal contributor author)
  6. AS Mount, NV Gohad*, D Hansen, K Hansen, MB Jonstone. 2010. Deposition of nanocrystalline calcite on surfaces by tissue and cellular biomineralization. United States Patent application, Pub. No.: US 2010/0150982 Al (*equal contributor author)
  7. NV Gohad*, NM Shah, AT Metters, AS Mount. 2010. Noradrenaline deters marine invertebrate biofouling when covalently bound in polymeric coatings. Journal of experimental marine biology and ecology. . 394(1-2): 63-73
    (*equal contributor author)
  8. NV Gohad, GH Dickinson, B Orihueladiza, D Rittschof, AS Mount. Visualizationof putative ion-transporting epithelia in Amphibalanus amphitrite using correlative microscopy:potential function in osmoregulation and biomineralization. Journal of experimental marine biology and ecology.  380(1-2): 88-98.

     

Neeraj V. Gohad

 


Grant Support:


Office of Naval Research (ONR)
Development of Marine Antifouling Coatings Utilizing a Fouling Deterrence strategy
Co-PI with Andrew S. Mount.

Defense University Research Instrumentation Proposal (DURIP)
Multidimensional 2-Photon Imaging System for the Investigation of Biofouling and Biomineralization Mechanisms in Marine Invertebrates
Co-PI with Andrew S. Mount

Air Force Office of Scientific Research (AFOSR)
Investigation of hemocyte adhesion and surface interactions
Co-PI with Andrew S. Mount

Professional Affiliations


  • American Society for Cell Biology (ASCB)
  • Society for Integrative and Comparative Biology (SICB)
  • Microscopy Society of America (MSA)
  • Materials Research Society (MRS)National Shellfisheries Association (NSA)
  • The American Association for the Advancement of Science (AAAS)
  • Sigma Xi: The Scientific Research Society