Neeraj V. Gohad, Ph.D.
Assistant Research Professor
319 Jordan Hall
- BS, Microbiology, University of Pune, 2003
- PhD, Biological Sciences, Clemson University, 2008
Systems Biology: Organisms-Cells-Molecules
- Using a variety of invertebrate model systems my research focuses on
understanding the evolution and function of G-Protein Coupled Receptors (GPCR)
in development and sensory perception. Utilizing biophysical methods like
2-Photon Microscopy and Non-linear optics combined with genomic and proteomic
approaches, my goal is to; Understand commonalities in spatial distribution and
function of sensory GPCRs across phylogeny. Study the convergent evolution
and/or evolutionary conservation of receptor classes and signal transduction
pathways and to elucidate the crucial role GPCRs play during development and
metamorphosis. I am keenly interested in holometabolic metamorphic induction from
mechanistic, functional (imaging) as well as genetic standpoints (proteomics
Adhesion: Interactions between Biolgogical Systems and Engineered Polymer Surfaces
Utilizing a cross-disciplinary approach I am working to understand the
interactions between engineered polymer substrates and biological systems at several
scales (appendages—cells—receptors). A wealth of fundamental and translational
information can be garnered by characterizing this interaction at the molecular
level. Particularly, I am interested in understanding the effect of bioactive
polymers on transducers such as G-Protein Receptor Kinases (GRKs) and
downstream effectors like cytoskeletal elements through receptor stimulation. The
goal of this work is to understand the physical as well as biological
mechanisms behind these interactions and apply this knowledge to the development
of novel biomaterials for maritime and biomedical applications.
- I am also interested in understanding adhesion from a physicochemical
standpoint, especially in marine larval bio-adhesives. I am currently working
to understanding the chemical nature and synthesis of these adhesives along
with the biology of adhesive production. As these bio-adhesives are found in
extremely small amounts in nature, conventional analysis techniques are unusable;
to this end I am employing quantitative biophysical techniques such as 2-Photon
Microscopy combined with and through collaborative efforts, chemical imaging
techniques like Coherent Anti-Stokes Raman Scattering Spectroscopy to better
understand chemical composition of these adhesives. Understanding under water
adhesion has the potential to inspire design of synthetic adhesive systems
which have a variety of applications in medicine and surgery.
- Recent advances in optical
microscopy have transformed neuroscience, cell biology, and biomedicine. I am
working to construct and adapt instrumentation for biophysical techniques like
2-Photon Microscopy and CARS for studying developmental and metabolic processes
in invertebrate model systems.
- Aldred, N., N. V. Gohad,
L. Petrone, B. Orihuela, B. Liedberg, T. Ederth, A. Mount, D. Rittschof and A.
S. Clare. 2013. Confocal microscopy-based
goniometry of barnacle cyprid permanent adhesive. The Journal of Experimental
Biology 216(11): 1969-1972. (This article was on the
Cover of Journal of Experimental Biology)
- NV Gohad, N Aldred, B Orihueladiaz, A Clare, D Rittschof, AS. Mount. 2012.
Observations on the settlement and cementation of barnacle (Balanus
amphitrite) cyprid larvae after artificial exposure to noradrenaline and
the locations of adrenergic-like receptors. Journal of Experimental Marine
Biology and Ecology Volume 416-417 (2012) 153–161
- Philip M. Imbesi, NV Gohad*, Michael J. Eller, Beatriz
Orihuela, Dan Rittschof, Emile A. Schweikert, Andrew S. Mount, and Karen L
Wooley. 2012. Noradrenaline-Functionalized Hyperbranched
Fluoropolymer-Poly(ethylene glycol) Cross-Linked Networks As Dual-Mode,
Anti-Biofouling Coatings. ACS-Nano VOL. 6-NO. 2, 1503-1512 (* equal
- N Pradhan, NV Gohad*, T Burg, S Birchfield, AS
Mount. 2011. Autonomous Tracking and Analysis of Barnacle Cyprid
Exploratory Behavior. Journal of experimental marine biology and ecology:
Volume 410, 15 December 2011, Pages 21-28 (*equal contributor
- AS Mount, NV Gohad*, AT Metters, NM Shah. 2011.
Method and compositions for Biofouling Deterrence (2009), United
States patent application, Pub. No.: US 201110123477 Al (*equal
- AS Mount, NV Gohad*, D Hansen, K Hansen, MB Jonstone. 2010.
Deposition of nanocrystalline calcite on surfaces by tissue and cellular
biomineralization. United States Patent application, Pub. No.: US
2010/0150982 Al (*equal contributor author)
- NV Gohad*, NM Shah, AT Metters, AS Mount. 2010. Noradrenaline
deters marine invertebrate biofouling when covalently bound in polymeric
coatings. Journal of experimental marine biology and ecology. . 394(1-2):
(*equal contributor author)
- NV Gohad, GH Dickinson, B Orihueladiza, D Rittschof, AS Mount.
Visualizationof putative ion-transporting epithelia in Amphibalanus
amphitrite using correlative microscopy:potential function in
osmoregulation and biomineralization. Journal of experimental marine biology
and ecology. 380(1-2): 88-98.