LIsa J. Bain
Associate Professor
Contact Information
Phone: 864-646-2187
Fax: 864-646-2277
Email: lbain@clemson.edu
Education
- Post-doctoral Associate, Fox Chase Cancer Center, Medical Oncology, 1998
- Ph.D., North Carolina State University, Toxicology, 1997.
- B.S., University of Georgia, Environmental Health Sciences, 1992.
Research Interests
- Understanding how altered gene expression in fish exposed to arsenic, chromium, or PAHs correlates with altered metabolism, reproduction, and development
- Determining ATP-dependent transport protein regulation and how this affects the metabolism, disposition, and elimination of drugs and toxicants
Research in my laboratory focuses on determining the mechanisms of protection and/or resistance organisms develop when exposed to chemicals. There are two specific projects that are currently underway. The first examines ATP-dependent transport proteins, specifically MRP1, MRP2, and MRP3, which are involved in the elimination of compounds from the liver, kidney, and intestine. This active elimination lowers both the concentration and the toxicity of the compound to the organism. We are elucidating which toxicants and drugs are substrates, what their normal physiological roles are, how these transporters are regulated, and the impacts of drug-drug, drug-toxicant, and toxicant-toxicant interactions.
The second project investigates altered gene expression in fish upon exposure to contaminants, such as chromium, arsenic, and polycyclic aromatic hydrocarbons. When organisms are exposed to toxicants, they alter the proteins they produce to deal specifically with the stress at hand. We are using cDNA arrays and real-time PCR to ascertain what genes are being altered, what the resulting consequence or cost is to the organism, and whether these genes can be used as biomarker probes to assess exposure to other contaminants. Our current project is specifically examining environmentally realistic concentrations of arsenic to ascertain its mechanism of developmental toxicity.
Selected Publications
- Roling JA., Bain LJ, Gardea-Torresdey J, Key PB, and Baldwin WS (2007) Using Fundulus heteroclitus arrays to monitor the effectiveness of Superfund site remediation at Shipyard Creek in Charleston, SC. Environmental Toxicology and Chemistry, 26:1205-1213.
- Gonzalez HO, Roling JA, Baldwin WS, and Bain LJ (2006) Physiological changes and differential gene expression in mummichogs (Fundulus heteroclitus) exposed to arsenic, Aquatic Toxicology, 77:43-52.
- Roling JA, Bain LJ, Gardea-Torresdey J, Bader J, and Baldwin WS (2006) Hexavalent chromium reduces larvae growth and alters gene expression in Fundulus heteroclitus, Environmental Toxicology and Chemistry, 25:2725-2733.
- Belinsky MG, Dawson PA, Shchaveleva I, Bain LJ, Wang R, Ling V, Chen Z-S, Grinberg A, Westphal H, Klein-Szanto A, Lerro A, and Kruh GD (2005) Analysis of the in vivo functions of Mrp3, Molecular Pharmacology, 68:160-168.
- Maples NL and Bain LJ (2004) Trivalent chromium alters gene expression in the mummichog (Fundulus heteroclitus), Environmental Toxicology and Chemistry, 23:626-631.
- Peterson JSK and Bain LJ (2004) Differential gene expression in anthracene-exposed mummichogs (Fundulus heteroclitus), Aquatic Toxicology, 66:345-355.
- Tribull TE, Bruner RH, and Bain LJ (2003) The Multidrug Resistance-Associated Protein 1 (MRP1/ABCC1) transports methoxychlor and protects the seminiferous epithelium from injury, Toxicology Letters, 142:61-70.
- Bain LJ and Feldman RA (2003) Altered expression of sulfotransferases, glucuronosyltransferases, and MRP transporters in FVB/mrp1-/- mice, Xenobiotica, 33:1173-1184.
Recent Courses
- ENTOX 430/630 Toxicology
- BIOSCI 461/661 Cell Biology
Previous Courses Taught
- Pathobiology
- Environmental Stressors in the Ecosystem
- General Biology
Graduate Students
- Benjamin Green
- Gia-Ming Hong
- Amanda Steffens
Professional Affiliations
- Society of Toxicology
- Society of Environmental Toxicology and Chemistry
- International Society for the Study of Xenobiotics