Lesly A. Temesvari

Alumni Distinguished Professor


Contact Information

Phone: 864 656-6387
FAX: 864 656-0435
Email: ltemesv@clemson.edu


Education

  • Postdoctoral Fellow, LSU Medical Center, 1993-1997
  • Ph.D. Biology, University of Windsor, 1993
  • B.S. Microbiology and Immunology(major) and Chemical Engineering (minor), McGill University, 1987

Research Interests

The overall goal of the research in Dr. Temesvari's laboratory is to understand the molecular mechanisms that govern vesicle trafficking and cell-cell interactions. Using state-of-art molecular and cellular techniques the members of her laboratory are approaching these questions in several ways. First, her laboratory is examining the role of (i) four Rab GTPases (master regulators of vesicle trafficking), (ii) several components of the PI 3-kinase signaling pathway, and (iii) lipids in the pathogenesis of the human protozoan parasite, Entamoeba histolytica. This parasite is the causative agent of amoebic dysentery and infects 50,000,000 people world-wide annually. Insight gained through these studies may lead to rational design of novel anti-Entamoeba agents. Second, her laboratory is examining the role of Rab8 in the regulation of secretion and cell adhesion during development using mammalian cells and the non-pathogenic amoeba, Dictyostelium discoideum. Rab8 is implicated in several disorders including melanoma and Alzheimer disease. These latter studies may provide important information regarding the evolution of vesicle trafficking pathways in eukaryotic cells, the role of vesicle trafficking during differentiation, and the molecular etiology of several disorders.


Selected Publications

  • 2008 Mittal, K., Welter, B.H., and Temesvari, L.A. Entamoeba histolytica: Lipid rafts are involved in adhesion of trophozoites to host extracellular matrix components. Exp. Parasitol. In Press (doi:10.1016/j.exppara.2008.06.003)
  • 2007 Dinolfo, J., Heifferon, B., and Temesvari, L.A. Seeing Cells: Teaching the Visual/Verbal Rhetoric of Biology. Journal of Technical Writing and Communication. 37, 395-417. Nominated as Paper of the Year by National Council of Teachers of English
  • 2006 Welter, B.H., Powell, R.R., Laughlin, R.C., McGugan, G., Bonner, M., King, A., and Temesvari, L.A. Entamoeba histolytica: Comparison of the role of receptors and filamentous actin among various endocytic processes. Exp. Parasitol. 113, 91-99.  Chosen as Cover for Experimental Parasitology June 2006 Issue
  • 2006 Powell, R.R., Welter, B.H., Hwu, R., Bowersox, B., Attaway, C., and Temesvari, L.A. Entamoeba histolytica: FYVE-finger domains, phosphatidylinositol 3-phosphate biosensors, associate with phagosomes but not fluid filled endosomes. Exp. Parasitol. 112, 221-231. Chosen as Cover for Experimental Parasitology April 2006 Issue
  • 2005 Welter, B.H., Powell, R.R., and Temesvari, L.A. A unique Rab GTPase, EhRabA, is involved in motility and polarization of Entamoeba histolytica cells. Mol. Biochem. Parasitol. 140, 161-173.
  • 2004 Laughlin, R.C., McGugan, G.C., Powell, R.R., Welter, B.H., and Temesvari, L.A. Characterization of lipid microdomains of Entamoeba histolytica. Infect. Immun. 72, 5349-5357. Highlighted in Nature Reviews Microbiology (Nat. Rev. Microbiol. 2, 770)
  • 2004 Powell, R.R., and Temesvari, L.A. Characterization of a family of Rab GTPases of Dictyostelium discoideum involved in cell-cell adhesion and regulated secretion during development. Microbiology 150, 2513-2525.
  • 2004 Welter, B.H., and Temesvari, L.A. A unique Rab GTPase, EhRabA, of Entamoeba histolytica, localizes to the leading edge of motile cells. Mol. Biochem. Parasitol. 135, 185-195.

Recent Courses

  • Biosc 461, 462 - Cell Biology, Cell Biology Laboratory
  • Biosc 871 - Advanced Topics in Cell Biology
  • Biosc 490 – Science Communication for Life Sciences Undergraduate Students
  • Biosc 871 - Science Communication for Life Sciences Undergraduate Students

Graduate Students

  • Ms. Terri Bruce, (Ph.D. Candidate, Biological Sciences)
  • Ms. Priyanka Shah (Ph.D. Student, Microbiology)
  • Ms. Rhonda Powell (M.S. Student, Microbiology)

Professional Affiliations

  • American Society for Microbiology
  • American Society for Cell Biology
  • Sigma Xi Honorary Research Society