Assistant Professor of Molecular Breeding and Faculty Scholar in Clemson University School of Health Research
Adjunct Assistant Professor in Dept. of Crop & Soil Sciences at Wash. State Univ., Pullman
Plant and Environmental Sciences Department, PeeDee Research and Education Center
Office: John B. Pitner Center, Pee Dee REC
Vita: Download CV
Ph.D. Crop Biotechnology
C.C.S. University, Meerut 2006
M.J.P. Rohilkhand University, Bareilly 2001
B.S. Biology and Chemistry
M.J.P. Rohilkhand University, Bareilly 1999
D.I.S.M. Diploma in Information Systems Management
Aptech, Chandausi 2000
Dr. Sachin Rustgi is currently affiliated as Assistant Professor in Molecular Breeding with the Department of Plant and Environmental Sciences at Clemson University, and Adjunct Assistant Professor with the Department of Crop and Soil Sciences at the Washington State University, Pullman. He holds a master’s degree in Botany from MJP Rohilkhand University, Bareilly and a Ph.D. degree in Crop Biotechnology from CCS University, Meerut. Dr. Rustgi’s research largely focuses on the improvement of nutritional value in the small grain cereals and legumes especially by elimination or detoxification of antinutritional elements and enhancement of health-promoting factors. In order to achieve these objectives, he has developed a multidisciplinary team of experts across different Colleges of the Clemson University, the Medical University of South Carolina and the Greenville Health System. His research has resulted in a number of high impact publications in internationally recognized journals including the Proceedings of the National Academy of Sciences of the United States of America, the Journal of Experimental Botany, Plant Molecular Biology, Heredity, PLoS One, and Nutrients, to name a few. In addition to it, Dr. Rustgi authored two monographic books on his research. In view of his academic contributions, he was awarded with the 2014 Faculty Excellence in Research Award of the Washington State University College of Agricultural, Human, and Natural Resource Sciences and invited to join as a member of the Wheat Initiative Expert Working Group on Wheat Quality for Processing and Health and the American Society for Nutrition.
My current research focus is bipartite: On one hand, I am involved in the understanding of basic plant processes such as transport of proteins and transfer RNA from the cytoplasm to the endosymbiotic organelles (mitochondria and chloroplast) in the plant cells, and the molecular mechanism of insect pest deterrence in plants. And on the other hand, I focus on the development of novel dietary therapies for the ‘gluten syndrome’ and other two major food allergens, peanuts, and soybean, as well as the development of novel RNA interference based pesticides for major insect pests in the Southeast US.
Total citations = 2633; h-index = 20; i10-index = 28
1. Gill US, Sun L, Rustgi S, Tang Y, von Wettstein D, Mysore KS (2018) Deciphering the molecular mechanism of phosphite mediated suppression of rust pathogens, Puccinia emaculata and Phakopsora pachyrhizi. The Plant Journal 93: 894-904.
2. Rustgi S, Boex-Fontvieille E, Reinbothe C, von Wettstein D, Reinbothe S (2018) The complex world of plant protease inhibitors: Insights into a Kunitz-type cysteine protease inhibitor of Arabidopsis thaliana. Communicative & Integrative Biology 11:e1368599.
3. Rustgi S, Gandhi, N, von Wettstein D, Ankrah N, Gemini R, Reisenauer P (2017) ‘Celiac-safe’ wheat genotypes: A target not beyond reach. Ann Wheat Newslet 63:85-88.
4. Rossig C, Gray J, Valdes O, Rustgi S, von Wettstein D, Reinbothe C, Reinbothe S (2017) Distinct HP30‐2, a mitochondrial PRAT protein for import of signal sequence‐less precursor proteins in Arabidopsis thaliana. J Integr Plant Biol doi: 10.1111/jipb.12555.
5. Yang MM, Gao X, Dong J, Gandhi N, Cai H, von Wettstein D, Rustgi S, Wen S (2017) Pattern of protein expression in developing wheat grains identified through proteomic analysis. Front Plant Sci 8:962.
6. Buhr F, Lahroussi A, Springer A, Rustgi S, von Wettstein D, Reinbothe C, Reinbothe S (2017) NADPH:protochlorophyllide oxidoreductase B (PORB) action in Arabidopsis thaliana revisited through transgenic expression of engineered barley PORB mutant proteins. Plant Mol Biol 94:45-59.
7. Rustgi S, Boex-Fontvieille E, Reinbothe C, von Wettstein D, Reinbothe D (2017) Serpin 1 and the water-soluble chlorophyll binding Kunitz protease inhibitor WSCP differentially regulate the activity of the cysteine protease RD21 (RESPONSIVE TO DESICCATION) during plant development in Arabidopsis thaliana. Proc Natl Acad Sci USA 114:2212–2217.
8. Rustgi S, Ankrah N, Brew-Appiah RAT, Sun Y, Liu W, von Wettstein D (2017) Doubled haploid transgenic wheat lines by microspore transformation, In: Bhalla P, Singh MB (eds.), Wheat Biotechnology, Methods and Protocols, Series: Methods in Molecular Biology, 1679, 213-234.
9. Rustgi S, von Wettstein D, Ankrah N, Ou X, Sun Y, Gemini R (2016) Quest for celiac-safe wheat. Ann Wheat Newslet 62:89-92.
10. Boex-Fontvieille E, Rustgi S, von Wettstein D, Pollmann S, Reinbothe S and Reinbothe C (2016) An ethylene-protected Achilles’ heel of etiolated seedlings for arthropod deterrence. Front Plant Sci 7:1246.
11. Boex-Fontvieille E, Rustgi S, von Wettstein D, Pollmann S, Reinbothe S, Reinbothe C (2016) Jasmonic acid protects etiolated seedlings of Arabidopsis thaliana against herbivorous arthropods. Plant Signaling and Behavior 11(8):e1214349.
12. Springer A, Kang C, Rustgi S, von Wettstein D, Reinbothe C, Pollmann S, Reinbothe S (2016) Programmed chloroplast destruction during leaf senescence involves 13-lipoxygenase (13-LOX). Proc Natl Acad Sci USA 113:3383–3388.
13. Balyan HS, Varshney RK, Roy JK, Prasad M, Kulwal PL, Kumar N, Rustgi S, Kumar A, Mir RR, Jaiswal V (2016) From development of molecular markers to marker-assisted breeding: A successful journey. In: the International Conference on Genomics and Translational Research in Crop Improvement, C.C.S. University, Meerut, Dec. 14-16, 2016.
14. Gray J, Rustgi S, von Wettstein D, Reinbothe C, Reinbothe S (2015) Common functions of the chloroplast and mitochondrial co-chaperones cpDnaJL (CDF1) and mtDnaJ (PAM16) in protein import and ROS scavenging in Arabidopsis thaliana. Commun Integr Biol 9: e1119343.
15. Boex-Fontvieille E, Rustgi S, Reinbothe S, Reinbothe C (2015) A Kunitz-type protease inhibitor regulates programmed cell death during flower development in Arabidopsis thaliana. J Exp Bot 66:6119-6135.
16. Rustgi S, von Wettstein D, Ankrah N, Brew-Appiah RAT, WenN, Mitchell SM, Gemini R, Reisenauer P (2015) Celiac-safe wheat genotypes: A dietary solution to the gluten-induced disorders. Ann Wheat Newslet 61:99-103.
17. Boex-Fontvieille E, Rustgi S, von Wettstein D, Reinbothe S, Reinbothe C (2015) Water-Soluble chlorophyll protein (WSCP) is involved in light-dependent herbivore resistance activation during greening of Arabidopsis thaliana. Proc Natl Acad Sci USA 112:7303–7308.
18. Rustgi S, von Wettetsin D (2015) Breeding barley ornamented with the novel agronomical attributes. Med Aromat Plants 4:e158.
19. Reinbothe S, Gray J, Rustgi S, von Wettstein D, Reinbothe C (2015) Cell growth defect factor 1 is crucial for the plastid import of NADPH:protochlorophyllide oxidoreductase A in Arabidopsis thaliana. Proc Natl Acad Sci USA 112: 838–5843.
20. Ou X, Zhuang T, Yin W, Miao Y, Zhang Y, Lin X, Xu C, von Wettstein D, Rustgi S, Liu B (2015) DNA methylation changes induced in rice by exposure to high concentrations of the nitric oxide modulator, sodium nitroprusside. Plant Mol Biol Rep 33:1428-1440.
21. Rustgi S, Pollmann S, Buhr F, Springer A, Reinbothe C, von Wettstein D, Reinbothe S, (2014) JIP60-mediated, jasmonate- and senescence-induced molecular switch in translation toward stress and defense protein synthesis. Proc Natl Acad Sci USA 111:14181-14186.
22. Bhullar R, Nagarajan R, Bennypaul H, Sidhu GK, Sidhu G, Rustgi S, von Wettstein D, Gill KS (2014) Silencing of a metaphase I-specific gene results in a phenotype similar to that of the Pairing homeologous 1 (Ph1) gene mutations. Proc Natl Acad Sci USA 111:14187-14192.
23. Yang C, Zhao L, Yang Z, Xu C, Zhang H, Wen S, Zhang C, Rustgi S, von Wettstein D, Liu B (2014) Evolution of physiological responses to salt stress in hexaploid wheat. Proc Natl Acad Sci USA 111:11882-11887.
24. Rustgi S, von Wettstein D, Ankrah N, Brew-Appiah RAT, Wen N, Mitchell SM, Gemini R, Reisenauer P, Brabb I (2014) Breeding celiac-safe wheat cultivars a future market class of wheat. Ann Wheat Newslet 60:143-146.
25. Hu L, Li N, Xu C, Zhong S, Lin X, Yang J, Zhou T, Yuliang A, Cao X, Zemach A, Rustgi S, von Wettstein D, Liu B (2014) Mutation of a major CG methylase in rice causes genome-wide hypomethylation, dysregulated genome expression, and seedling lethality. Proc Natl Acad Sci USA 111:10642-10647.
26. Rustgi S, Matanguihan J, Mejías J, Gemini R, Osorio C, Wen N, Ankrah N, Brew-Appiah R, Murphy KM, von Wettstein D (2014) Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance. PLOS One 9:e100998.
27. Rustgi S (2014) Discussion with Assistant Research Professor Sachin Rustgi on the genetic modification of wheat to make it safe for celiacs. Journal of Gluten Sensitivity 13(2):11-14.
28. Mejias JH, Lu X, Osorio C, Ullman JL, von Wettstein D, Rustgi S (2014) Analysis of wheat prolamins, the causative agents of celiac sprue, using reversed phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Nutrients 6:1578–1597.
29. Zhang H, Bian Y, Gou X, Zhu B, Xu C, Qi B, Li N, Rustgi S, Zhou H, Han F, Jiang J, von Wettstein D, Liu B (2013) Persistent whole-chromosome aneuploidy is generally associated with nascent allohexaploid wheat. Proc Natl Acad Sci USA 110:3447-3452.
30. Gupta PK, Rustgi S, Mir RR (2013) Array-based high-throughput DNA markers for cereal genetics and genomics. In: Gupta PK, Varshney RK (Eds.), Cereal Genomics II, Springer, ISBN: 978-94-007-6401-9.
31. Kumar N, Rustgi S (2013) Agronomically relevant traits transferred to major crop plants by alien introgressions. In: Pratap A, Kumar J (Eds.), Alien Gene Transfer in Crop Plants: Innovations, Methods and Risk Assessment (Vol. I), Springer, New York, USA, ISBN: 978-1-4614-8584-1.
32. Rustgi S, Shafqat MN, Kumar N, Ali ML, Dweikat I, Baenziger P, Gill KS (2013) Genetic dissection of yield and its component traits using high-density composite map of wheat chromosome 3A: Bridging gaps between QTLs and underlying genes. PLoS ONE 8: e70526.
33. Rustgi S, von Wettstein D, Ankrah N, Mejias JH, Brew-Appiah RAT, Wen S, Wen N, Osorio C, Gemini R, Reisenauer P, Mohan J, Brabb I (2013) A natural dietary-therapy for gluten intolerance, sensitivity and allergenicity. Ann Wheat Newslet 59:151-155.
34. Rustgi S (2013) Engineering wheat genotypes compatible for gluten sensitive, allergenic and intolerant individuals. Int J Plant Biol Res 1:1003.
35. Brew-Appiah RAT, Ankrah N, Liu W, Konzak CF, von Wettstein D, Rustgi S (2013) Generation of doubled haploid transgenic wheat lines by microspore transformation. PLoS ONE 8:e80155.
36. Zhang H, Bian Y, Gou X, Dong Y, Rustgi S, Zhang B, Xu C, Li N, Qi B, Han F, von Wettstein D, Liu B (2013) Intrinsic karyotype stability and gene copy number variations may have laid the foundation for tetraploid wheat formation. Proc Natl Acad Sci USA 110:19466-19471.
37. Rustgi S (2013) Bringing barley back in crop rotation by breeding for imidazolinone resistance. Med Aromat Plants 2:e148.
38. Bennypaul HS, Mutti JS, Rustgi S, Kumar N, Okubara PA, Gill KS (2012) Virus-induced gene silencing (VIGS) of genes expressed in root, leaf and meiotic tissues of wheat, Funct Integr Genomics 12:143-156.
39. Osorio C, Wen N, Gemini R, Zemetra R, von Wettstein D, Rustgi S (2012) Targeted modification of wheat grain protein to reduce the content of celiac causing epitopes, Funct Integr Genomics 12:417-438.
40. Rustgi S, von Wettstein D, Ankrah N, Brew-Appiah RAT, Wen S, Wen N, Osorio C, Gemini R, Reisenauer P, Lu X, Mejias JH (2012) Engineering wheat for celiac patients. Ann Wheat Newslet 58:248-253.
41. Wen S, Wen N, Pang J, Langen G, Brew-Appiah RAT, Mejias JH, Osorio C, Yang MM, Gemini R, Moehs CP, Zemetra RS, Kogel KH, Liu B, Wang X, von Wettstein D, Rustgi S (2012) Structural genes of wheat and barley 5-methylcytosine DNA glycosylases and their potential applications for human health. Proc Natl Acad Sci USA 109:20543-20548.
42. Riar DS, Rustgi S, Burke IC, Gill KS, Yenish JP (2011) EST-SSR development from 5 Lactuca species and their use in studying genetic diversity among L. serriola biotypes, J Heredity 102:17-28.
43. Lee H, Rustgi S, Kumar N, Burke IC, Yenish JP, Gill KS, von Wettstein D, Ullrich SE (2011) Single nucleotide mutation in the barley acetohydroxy acid synthase (AHAS) gene confers resistance to imidazolinone herbicides. Proc Natl Acad Sci USA 108:8909-8913.
44. Bandopadhyay R, Rustgi S, Chaudhuri RK, Khurana P, Khurana JP, Tyagi AK, Balyan HS, Houben A, Gupta PK (2011) Use of methylation filtration and C0t fractionation for analysis of genome composition and comparative genomics in bread wheat. J Genet Genomics 38:315-325.
45. Rustgi S, Balyan HS, Gupta PK (2011) MSL and SNPL: Softwares for in silico mining of SSRs and SNPs. Int J Biotechnol Biosci 1:353-355.
46. Baenziger PS, Dweikat I, Gill K, Eskridge K, Berke T, Shah M, Campbell BT, Ali ML, Mengistu N, Mahmood A, Auvuchanon A, Yen Y, Rustgi S, Moreno-Sevilla B, Mujeeb-Kazi A, Morris RM (2011) Understanding grain yield: It is a journey, not a destination. Checz J Genet Plant Breed 47:S77-S84.
47. von Wettstein D, Rustgi S, Kannangara CG, Ankrah N, Brew-Appiah RAT, Wen S, Wen N, Gemini R, Brueggeman R, Reisenauer P, Gill KS, Moehs CP, Liu B, Pang J, Wang X, Langen G, Kogel KH (2010) Multipronged approach to develop nutritionally improved celiac safe wheat varieties. Ann Wheat Newslet 56:261-264.
48. Rustgi S, Bandopadhyay R, Balyan HS, Gupta PK (2009) EST-SNPs in bread wheat: Discovery, validation, genotyping and haplotype structure. Checz J Genet Plant Breed 45:106-116.
49. Sidhu GK, Rustgi S, Shafqat MN, von Wettstein D, Gill KS (2008) Fine structure mapping of a gene-rich region of wheat carrying Ph1, a suppressor of crossing over between homoeologous chromosomes. Proc Natl Acad Sci USA 105:5815-5820.
50. Gupta PK, Rustgi S, Mir RR (2008) Array-based high-throughput DNA markers for crop improvement. Heredity 101:5-18.
51. Mir RR, Rustgi S, Sharma S, Singh R, Goyal A, Kumar J, Gaur A, Tyagi AK, Khan H, Sinha MK, Balyan HS, Gupta PK (2008) A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute. Euphytica 161:413-427.