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Kennedy Center

Projects

Overview

The Kennedy Center conducts original applied and basic research on waterfowl, wetlands, and other wetland-dependent wildlife. Our research addresses the issues managers face, and we provide solutions to improve the management of wetlands and waterfowl on public and private lands.

Quantifying Habitat Use by Secretive Marsh Birds in Antebellum Rice Fields Using Drones and Autonomous Recording Units in Coastal South Carolina

Akshit R. Suthar
Doctoral Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Secretive marsh birds, such as rails, bitterns, and gallinules, are notoriously elusive and well-camouflaged, presenting significant challenges for study using traditional methods. These birds inhabit dense vegetation in wetland habitats, making observation and study difficult. Antebellum (pre-U.S. Civil War) rice field impoundments provide essential habitat for many species of secretive marsh birds in coastal South Carolina. These rice fields, developed between the 1600s and 1800s, were primarily managed by the labor of enslaved people during the colonial era. Rice culture declined post-Civil War, and recent mapping efforts show it spans about 95,000 hectares. Currently, many of these fields are primarily managed for wintering waterfowl. Recent studies suggest constant water level management creates higher sites within an impoundment and can develop patches of saltgrass, clump cordgrass, and salt meadow cordgrass (Distichlis spicata, Sporobolus bakeri, and Sporobolus pumilus), providing suitable cover and nesting habitat for secretive marsh birds.

Our research aims to 1) quantify habitat use by these birds within the antebellum rice fields of coastal South Carolina and 2) Design innovative techniques for monitoring them. We employed Autonomous Recording Units (ARUs) for Passive Acoustic Monitoring (PAM) to capture the call activities of secretive marsh birds. ARUs are less invasive and reduce time and labor costs. We used Audiomoth recorders to monitor king rail (Rallus elegans), clapper rail (Rallus crepitans), Virginia rail (Rallus limicola), sora (Porzana carolina), black rail (Laterallus jamaicensis), American bittern (Botaurus lentiginosus) and least bittern (Ixobrychus exilis) during their breeding season from March to mid-June when they are notably more vocal. Our recording schedule involved capturing audio for one minute every three minutes from 5:45 PM to 9 AM for three days, aligning with the peak activity periods of the focal species.

We developed a low-cost methodology using drones to deploy and retrieve ARUs mounted on lightweight, multi-terrain floating platforms from inaccessible areas. This approach aimed to overcome the limitations of traditional deployment methods, such as data bias from unapproachable large managed and unmanaged rice field impoundments. We maintain a 200-meter distance between each ARU, effectively covering entire habitats with less time, effort, and human resources. This method allowed us to deploy ARUs in the center of impoundments and far, inaccessible and less disturbed areas, which are crucial for monitoring potential habitats of species like the black rail.

For data analysis, we utilized the open-source Artificial Intelligence model called BirdNet, developed by Cornell Lab. Data recorded in .wav format was fed into the model, which analyzed each three-second spectrogram against eBird checklists and BirdNet algorithms, providing species identification with confidence intervals. Last season, we deployed 130 ARUs, surveyed 29 rice field impoundments, recorded approximately 119,000 files, and logged around 2,000 hours of recordings. Moving forward, we plan to expand our survey efforts, double the number of impoundments surveyed, and analyze the collected data to understand better the habitat use and preferences of secretive marsh birds in these historically significant landscapes.
This research underscores the importance of integrating advanced technologies, like drones and ARUs, in secretive marsh bird monitoring and conservation. The innovative methodologies developed here enhance our understanding of secretive marsh bird populations and offer scalable habitat monitoring solutions in challenging environments.

  • Image 1: Deploying and retrieving ARUs mounted on top of a designed multi-terrain lightweight floating platform in inaccessible areas using a drone.
  • Image 2: Successfully deployed ARUs with a multi-terrain lightweight floating platform, ready to record marsh birds.
  • Image 3: ARUs deployed using kayaking and on foot-based traditional methods in approachable areas.
  • Image 4: Typical federally endangered black rail habitat with high marsh and dense vegetation cover within antebellum rice fields.
  • Image 5: King rail crossing impoundments via managed rice field dikes.
  • Image 6: Least bittern spotted in a managed rice field canal.
  • Image 7: Virginia rail image captured by drone in the middle of a managed rice field impoundment.
  • Image 8: Data analysis process flowchart using the BirdNET AI model.

Akshit with his recording equipment

marsh bird in a lake

Understanding Waterfowl Habitat Relations with Antebellum Rice Fields Using Drones in Coastal South Carolina

Akshit R. Suthar
Doctoral Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Coastal South Carolina’s antebellum (Pre-Civil War) rice field impoundments play a crucial role in waterfowl conservation. The cultivation of rice began in the 1600s, deeply intertwined with the history of enslavement and the transatlantic enslaved laborer trade. Enslaved Africans brought their knowledge and technologies of rice cultivation from West Africa to colonial South Carolina, constructing extensive dikes and canal systems by hand to create rice field impoundments. These transformations converted tidal swamps and hardwood bottomland forests into highly managed agricultural systems. Although rice cultivation declined post-Civil War, the loss of labor as former enslaved persons were freed, and subsequent hurricanes and tropical storms caused extensive damage to the agricultural rice areas. Attempts to rebuild were unsuccessful due to costs, other factors, and increasing market competition from rice produced in Texas, Louisiana, and Arkansas. Today, approximately 95,000 hectares of antebellum rice fields are mapped and categorized as Tidal Functional, Tidal Broken, and Inland (Figure 1), with many managed for waterfowl. This habitat faces significant threats from sea level rise and climate change, exacerbating the degradation of rice field infrastructure through increased tidal amplitudes and more frequent tropical storms (Figure 2). There is ongoing debate about the impact of impoundments on wetlands and waterfowl habitats. Some argue that managed impoundments are more beneficial, while others believe that tidal wetlands would suffice if impoundments didn't exist. This highlights the complexity of conservation planning. Recent research underscores the high value of antebellum rice field impoundments for waterfowl. Accurate quantification of waterfowl utilization, along with understanding the biological and environmental factors driving high abundance, is essential for efficient conservation, management, and restoration of these habitats.

Drones are increasingly utilized in wildlife surveys, providing an aerial perspective of the landscape and overcoming accessibility and visibility challenges, which can be particularly limiting in waterfowl surveys. Our objectives were to 1) estimate waterfowl abundance by species and guilds within these rice fields using drone 2) investigate the relationships between waterfowl and impoundment characteristics, such as average water depth and vegetation types, to understand habitat preferences 3) evaluate the efficacy of drones for future waterfowl research. We conducted preliminary surveys at the Tom Yawkey Wildlife Center, Georgetown, during February and December 2023, utilizing a DJI Mavic 3T enterprise drone (Figure 3). We conducted aerial surveys of Tidal Functional rice field impoundments (N=2) (Blackout and Penfold) to estimate waterfowl abundance and species richness and collected high-resolution images of impoundments for modeling habitat characteristics using an ArcGIS Deep Machine Learning model. We conducted a traditional ground-based survey to compare both methodologies to count waterbirds.

Our findings reveal that the total average waterbird counts obtained from drone surveys were significantly higher, with an average of 2,202 birds counted. This is 347.21% higher compared to the average of 492 birds counted during ground surveys (Figure 4). Additionally, drone surveys were able to identify an average of 12 species, which is 8.7% higher than the 11 species identified through ground surveys (Figure 5). Our study demonstrates the effectiveness of drones for accurately identifying and quantifying waterbirds compared to ground surveys, especially in areas with large bird populations. Using ArcGIS Deep Machine Learning model, we classified and categorized impoundments in emergent and submerged vegetation, open water, and dikes. Kernel density estimation was used to analyze waterbird habitat selection, while Poisson regression models determined the impact of impoundment’s hydrological variables on waterfowl abundance (Figure 6). We found % submerged vegetation has a strong positive impact on waterfowl abundance across all species. % open water has positive impact with varying significance, average water level (cm) has positive impact with varying significance, % emergent vegetation has small or non-significant impact and, impoundment size (ha) has less clear impact, with some species showing negative or non-significant effects (Figure 7)). Integrating thermal and RGB images improved detection probabilities (Figure 8), demonstrating the added benefits of thermal imaging for waterfowl surveys. Our study underscores the importance of antebellum rice field impoundments in maintaining waterbird habitats and provides essential insights for conserving, managing, and restoring antebellum rice fields by focusing on key habitat features crucial for supporting waterbird populations. emphasize the need for advanced management strategies to conserve these critical waterfowl habitats amidst ongoing environmental changes.

  • Figure 1: Map showing the location and geographic extent of identified antebellum rice fields in South Carolina.
  • Figure 2: Damaged earthen dike and wooden trunk due to sea level rise and tropical storm events.
  • Figure 3: DJI Mavic 3T drone with RGB and thermal camera.
  • Figure 4: Graph compares the average species-wise waterbird mean counts by drone-based aerial and traditional ground survey methods.
  • Figure 5: Graphs show mean total average waterbird counts and species richness obtained from drone-based aerial and traditional ground survey methods.
  • Figure 6: Maps shows impoundment habitat classification using deep machine learning and Kernel density estimation to understand waterbird habitat selection within impoundments.
  • Figure 7: Graph shows the impact of impoundment’s hydrological characteristics on selected species' abundance.
  • Figure 8: Integration of thermal images with RGB images to improve detection probability.

above view of waterfowl swimming in marsh

alt

density map of waterfowl

Biotic and Spatial Factors Driving Microplastic Abundance in the Gizzard of Green- winged Teal (Anas c. carolinensis) in Historic Antebellum Rice Fields in South Carolina

Aruã Yaym de Castro Ferreira
Master’s Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Cente

Derived from the polymerization of monomers, plastics are synthetic organic polymers whose large-scale production began in 1950. Improper plastic waste management has become a rising problem facing humanity as oceans, waterways, and terrestrial areas all become choked with plastic waste products. Microplastics are introduced into the environment via several anthropogenic activities, such as cloth washing, use of hygiene care products like exfoliants, and industrial by-products (Velis et al., 2017) (Figure 1). Consequently, there has been an increasing concern about microplastics as an emerging pollutant. Microplastics are plastic particles ranging from 1 μm – 5 mm in size, though the absolute lower size limits vary. These microplastics originate as primary or secondary plastics. Primary microplastics are manufactured and include products like exfoliating beads in cosmetic cleansers, plastic microspheres used in biomedical and life sciences research, and industrial abrasives. Secondary microplastics result from the deterioration and fragmentation of larger microplastic pieces into smaller fragments.

Sources, pathways, transformations and fate of marine litter

Microplastics are a significant threat to both wildlife and humans. The ingestion of microplastics by organisms can result in physiological and behavioral impairments. Additionally, microplastics can absorb and transport toxic compounds (i.e., PFAS), serving as a vector for these substances within food webs. Coastal ecosystems are particularly vulnerable to microplastics as they receive high input from urban runoff, wastewater discharge, and industrial effluents. Microplastic accumulation in coastal environments can threaten the biodiversity and function of these systems, which are crucial for migratory species and local communities.

Historic antebellum rice fields (HARF) in Coastal South Carolina (SC) with origins in the transatlantic slave trade in the 1600s are a crucial habitat for waterfowl. Nowadays, ~33,000 acres of tidal HARFs remain functional and provide habitat for a myriad of waterfowl species. According to Masto et al. (2023) and Hanks et al. (2021), these rice fields are highly utilized by wintering dabbling ducks. Approximately 30% of all dabbling ducks in the Atlantic Flyway winter in the South Atlantic region, particularly in coastal SC. Thus, tidal functional HARFs in SC are a crucial habitat for wintering dabbling ducks in the Atlantic Flyway.

Rice Field

Currently these critical wetlands are under threat from microplastics. Adjacent anthropogenic development has the potential to deposit microplastics in these unique systems, threatening the survival of waterfowl that depend on HARFs. Given these threats, I aim to investigate the presence and concentration of microplastics in the gizzard of Green-winged Teal (GWTE) as a bioindicator for microplastic contamination in SC’s HARFs.

The goal of this research is comprised of three objectives: 1) Assess the presence and concentration of microplastics in the gizzard of GWTE in SC’s HARFs. GWTE was the most harvested species (n=641) in the SC Department of Natural Resources Waterfowl Management Areas in the 2024-25 hunting season. Consequently, it is an optimal surrogate species, since hunters may be the terminal link in the microplastic trophic pathway. Microplastic presence and concentration will be assessed utilizing chemical digestion with potassium hydroxide (KOH) and hydrogen peroxide (H2O2) paired with the Agilent 8700 Laser Direct Infrared (LDIR) chemical imaging device (Figure 2). 2) Utilize a linear mixed effects model to determine whether microplastic concentration, microplastic type, and polymer type is affected by mass, sex, predominant species, location, watershed, urbanization, and population density. 3) Compare the performance of the Agilent 8700 LDIR to a light microscope for identifying microplastic types and measuring microplastic concentration.

Sample analysis methodology

Green-winged Teal (Anas carolinensis)

At this point in time, I am chemically digesting the gizzard contents and will begin chemical imaging shortly. Nonetheless, given the findings of Gray et al. (2018) and the tidal nature of coastal HARFs, I predict microplastics will be present in the gizzard of GWTEs. Additionally, I predict synthetic particle polymer types to be congruent with the findings of Boucher et al. (Unpublished), where rubber and polyamide are highly abundant. Furthermore, I predict sex, predominant prey species, urbanization, and population density will significantly influence microplastic abundance. Finally, I predict the light microscope will identify trends in the abundance of microplastics, but it will underestimate overall abundance.

This research effort would not have been possible without the support of the SC Department of Natural Resources, Southeast Mitigation, Mississippi State University, Nemours Wildlife Foundation, The Wildlife Society – Wetlands Working Group, and the Whitmire Laboratory..

References

  • Boucher, M. N. Unpublished data. Regional Alligator Diet and Ecotoxicology of Contaminant of Emerging Concern in American Alligator (Alligator mississippiensis). Clemson University, SC
  • Gray, A. D., Wertz, H., Leads, R. R., & Weinstein, J. E. 2018. Microplastic in two South Carolina Estuaries: Occurrence, distribution, and composition. Marine Pollution Bulletin, 128, 223-233.
  • Hanks, R. D., Baldwin, R. F., Folk, T. H., Wiggers, E. P., Coen, R. H., Gouin, M. L., ... & Fields-Black, E. L. 2021. Mapping antebellum rice fields as a basis for understanding human and ecological consequences of the era of slavery. Land, 10(8), 831.
  • Masto, N. M., Hsiung, A. C., Kaminski, R. M., Ross, B. E., Kneece, M. R., Wilkerson, G. L., ... & Anderson, J. T. 2023. Waterbird–habitat relationships in South Carolina: implications for protection, restoration, and management of coastal and inland wetlands. Restoration Ecology, 31(7), e13956.
  • Velis, C., Lerpiniere, D., & Tsakona, M. 2017. Prevent Marine Plastic Litter - Now! An ISWA facilitated partnership to prevent marine litter, with a global call to action for investing in sustainable waste and resources management worldwide. Report prepared on behalf of the International Solid Waste Association (ISWA). An output of ISWA Marine Litter Task Force. ISWA September 2017. Vienna, pp.75..

Will Oysters Improve Restoration Outcomes at a Little Edisto Island Salt Marsh?

Christopher Pettengill
Doctoral Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Oysters are one of the most prevalent sessile organisms on the eastern seaboard of the United States. Oyster beds can be deployed in areas experiencing rapid shoreline erosion, but they are not a permanent fixture of coastal mitigation banks. Stakeholders are interested in the contributions of oysters to meeting restoration goals in coastal habitats, such as improvements in water quality and providing habitat structure for wildlife. Research is needed to understand better the impacts of incorporating oysters into salt marsh restoration. Christopher is investigating how the addition of oyster structures (wooden stakes, wireframes) to salt marsh restoration sites can enhance habitat quality and provide economic benefits to the site. Examples of these ecosystem services include providing habitat for fish and invertebrates (which provides food for aquatic birds) and biofiltration (for which they are interested in nitrate and phosphate reduction).

Christopher’s primary research objectives are:

  1. Calculate rates of biofiltration of oysters at the restoration site and compare it with rates observed at reference sites (those with oyster reefs and those without)
  2. Determine the spatial extent across which we observe the benefits of oyster ecosystem services (biofiltration and habitat provision)
  3. Record changes in community composition of fish and invertebrates at the restoration site following the addition of oysters to the site. Compare these communities with those seen at reference sites with varying oyster density.
  4. Determine how oyster abundance at the restoration site impacts habitat usage from waterfowl, shorebirds, and wading birds.
  5. Compare the recruitment and growth rates of oysters on different substrate types introduced to the restoration site.

Christopher’s hypotheses for each research objective are:

  1. The concentration of phosphorus and nitrate is lower at reference sites with a higher density of oysters. The concentration of phosphate and nitrate will decrease following the addition of oysters to the restoration site.
  2. The spatial extent of oyster ecosystem services at the restoration site will be much lower when compared to sites with well-established oyster reefs within the time frame of the study. If the site were monitored for a more extended period (ten years or more), we would likely see a more significant overall effect of oyster addition.
  3. Fish and invertebrate community composition at reference sites with high oyster density will be different from sites with low oyster density. The current community assemblage of the restoration site will be closer in composition to reference sites with low oyster density. As they continue to monitor the impoundments following the addition of new oyster cultch (shell, stone, or other structures that support oyster recruitment), Christopher expects that the biotic communities (invertebrates and fish) and water quality parameters (nitrate and phosphate concentration) will more closely resemble reference sites with oysters.
  4. Bird habitat usage will increase at the restoration site over time due to changes outside of oyster habitat addition, such as hydrologic alteration. The effects of increased oyster habitat may not be significant.
  5. No significant differences between oyster growth rates on tested oyster cultch materials (wooden stakes, wireframe reef structure) is expected. Community composition is also not expected to be significantly different between structure types.

The restoration site where the study is being conducted is located at Little Edisto Island, South Carolina. Southeast Mitigation LLC (funding source and partner) is restoring natural tidal flux to a set of six saltwater impoundments at this location by removing sections of the surrounding berms.

Christopher is comparing the habitat conditions present at the restoration site currently with multiple reference sites also found on Little Edisto Island and Edisto Island, which he places into categories depending on the density of oysters and oyster beds present at the sites.

Methods:

Each month, Christopher records water quality parameters (water temperature, salinity, conductivity, pH, dissolved oxygen concentration). He takes water samples, which he filters using a Millex brand 45-um filter unit, and processes in the lab to record concentrations of phosphate and nitrate. Christopher monitors bird activity at each of the impoundments during monthly surveys. For fish sampling, Christopher uses minnow traps (six per impoundment), crab traps (one per impoundment), a fifty-foot seine net (five sweeps per impoundment), and throwing an eight-foot diameter cast net (ten cast net throws per impoundment). Invertebrate surveys involve taking sediment core samples using a PVC pipe sediment trap, as well as a D-net. Christopher samples invertebrates along a transect from the most upland position in the impoundment (that is covered by water at high tide) to the deepest point (that is accessible) of the impoundment. The same survey methods are also used at each reference site where surveys of that type are possible. In a separate experiment taking place at the restoration site, Christopher will be comparing the growth and colonization rates of oysters on wireframes and wooden stakes. The goal is to determine which of the oyster structure types provides the greatest oyster growth and recruitment relative to the cost of deploying the structures. Stakeholders from Southeast Mitigation will be using the more efficient of these two methods to deploy oysters at a much larger restoration site, where they plan on creating one hundred acres of oyster habitat.

Preliminary results – Prior to restoration actions

Pre-restoration conditions at the site show that wading birds, shorebirds, and wetland-associated songbirds use the impoundments at the Little Edisto Restoration site, mainly when large numbers of birds descend onto exposed mudflat habitat at low tide. Impoundments (and portions of impoundments) that have been unconnected to any tidal influence show few signs of bird activity outside of grassland songbirds. Waterfowl are currently essentially absent from the restoration site, outside of one male-female pair of hooded mergansers present in one of the impoundments with permanent water during a winter survey. Plant communities at the restoration site reflect what are traditionally considered salt marsh plant community assemblages, with Sporobolus alterniflorus, Salicornia depressa, and Distichlis spicata being the three dominant species. Water quality at the restoration site varies slightly across impoundments. Still, we are expecting to see a more significant effect of season on nitrate and phosphate concentrations, with higher concentrations being detected during summer than in winter.

marsh landscape

oyster map of marsh

Occurrence of Cavities and Cavities Suitable for Nesting Wood Ducks Across South Carolina Forest Types

Cindy L. Von Haugg
M.S. Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Wood ducks (Aix sponsa) are common year-round residents of wetlands throughout the Southeastern United States. Evidence suggests that >90% of the North American wood duck population nests in tree cavities rather than in artificial nest boxes. However, few studies exist on the occurrence of natural tree cavities, particularly across their southern breeding range. We aimed to determine forest and tree characteristics indicative of cavities suitable for nesting wood duck hens to direct future studies to areas most likely to have cavities. We surveyed 20-m radius plots within the five dominant forest types of South Carolina (n = 166) on private and federally managed land and measured and inspected 4,633 trees >22 cm diameter at breast height (DBH) for cavity presence and suitability. We identified 225 potential cavities, 156 cavities, and 31 cavities suitable for nesting wood ducks. We found total cavity and suitable cavity densities (no./ha) at our study sites were greatest in oak (Quercus spp.), gum (Nyssa spp.), and cypress (Taxodium spp.) (19.35 ± 20.27, 4.20 ± 5.12) stands, followed by oak and hickory (Carya spp.) (8.37 ± 11.42, 1.36 ± 2.98), oak and pine (Pinus spp.) (4.90 ± 7.15, 1.17 ± 2.80), loblolly pine (P. taeda) (2.10 ± 4.42, 0.23 ± 1.34), and longleaf pine (P. palustris) (1.40 ± 3.57, 0.23 ± 1.34), which was consistent with densities at both sites individually. The best-fit model for cavity presence showed a significant positive effect for site index, DBH, and stand age and a minor negative effect for tree density. Results for suitability cavity presence also showed a significant positive effect for DBH and stand age, a significant negative effect for tree density, and no effect for basal area. We used an optimized hot spot analysis in ArcGIS to narrow down our total sample area using our findings to areas with 90% (73 ha), 95% (56 ha), and 99% (732 ha) confidence for suitable cavity occurrence, which amounted to 0.001% (73 ha), 0.001% (56 ha), and 0.008% of our total sample area (89,559 ha), respectively. This understanding of the relative abundance of cavities and cavities suitable for wood duck nesting and the identification of tree and stand forest metrics that influence the occurrence of cavities and suitable cavities promotes efficient management of nesting wood ducks, forest, and timber harvest practices.

Figure 1. Densities of cavities (F4, 4624 = 22.83; P < 0.0001) and suitable cavities (F4, 4624 = 4.29; P = 0.0018) for wood ducks in 166 randomly selected 20-m plots by forest type (n = 5). Plot surveys were conducted in stands >50 years old at Hobcaw Barony and Francis Marion National Forest, South Carolina, USA. Error bars indicate 95% confidence intervals, and significant differences are represented with different letters.

Figure 2. Heat map using estimates of relative abundance to develop optimized hot spots for areas with >95% and >99% confidence intervals for highest and lowest cavity occurrence probabilities. The abundance estimates were derived from forest surveys at randomly selected 20-m plots, stratified by forest type, at Francis Marion National Forest (left) in Berkeley and Charleston Counties, and Hobcaw Barony (right) in Georgetown County, South Carolina, USA, from February through July 2022.

map of tree cavities in sc

students taking measurements in the forest

Using Camera Traps to Monitor Seasonal and Diet Activity Patterns of Aix Sponsa (Wood Duck) During the Breeding Season

Cindy L. Von Haugg
M.S Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

The reproductive ecology of cavity-nesting Aix sponsa (Wood Duck) has long gone understudied due to the secretive nature of this species and the inability to estimate the vital rates of the population. Trapping and monitoring individual activity has been successfully used to uncover the behavior and habitat selection of numerous species. However, migratory species add additional difficulty to diagnosing the proper timing of trapping efforts. Uncovering successful trapping techniques is required to maximize research efforts and increase our understanding of cavity-nesting Wood Ducks. Moreover, timing trapping effort is fundamental to successfully trapping locally nesting Wood Duck hens. Between 11 January and 30 June 2023, we monitored trap sites (n = 41). We collected trail camera images with Wood Ducks (n = 2,271 frames) to evaluate seasonal and diel activity patterns of hen, drake, and hatch-year Wood Ducks in coastal South Carolina. Our analysis of images of hen (n = 1,641), drake (n = 2,358), and hatch-year (n = 341) Wood Ducks showed that the occurrence of each differed among months, and the highest densities of hens occurred in January, February, and May. Our results suggest setting traps no earlier than 1 March to increase the chance of trapping a locally nesting hen. Our estimates also suggest the pre-breeding phase occurred from mid-January to late March; the first nesting attempts occurred from early March to late April, and the second nesting attempts began around late April. Wood Duck (96.5%) and hen-specific (96.3%) activity were highest during diurnal periods, suggesting trap site visits should be avoided at this time to minimize disturbance. Using these results as guidelines for future trapping efforts could greatly improve the trapping success of Wood Duck hens during the breeding season, thus increasing our understanding of the reproductive ecology of cavity-nesting Wood Ducks and best-informing management decisions.

Figure 1. The four trap phases, one week per phase, were used to accustom the wood duck (Aix sponsa) to the trap site over 28 days: 1) bait only; 2) the wire was positioned linear across the trap site between the observed avenue of approach and the bait; 3) the wire was positioned in a circle or rectangle, if it was a rectangular trap, with the bait placed inside; and 4) the trap was set. The trap entrance was configured into a funnel 20 cm at the narrowest point and 1.9 cm, heavy-duty nylon mesh, poultry netting, or top panels, if it was a rectangular trap, were zip-tied across the top (North and Hicks 2017). The set trap was approximately 2.5 m in diameter or length. Trapping efforts were conducted from 11 January to 30 June 2023 at Ernest F. Hollings ACE Basin National Wildlife Refuge in Colleton, Charleston, and Beaufort Counties, Waccamaw National Wildlife Refuge in Georgetown, Horry, and Marion counties, Santee National Wildlife Refuge in Clarendon County, Clemson University’s Pee Dee Research and Education Center in Darlington and Florence counties, and privately-owned Witherspoon Island in Darlington County, South Carolina, USA.

four camera feeds of a lake

graph of hatching time of different birds

Method for Evaluating and Measuring Cavity Suitability for Nesting Wood Ducks

Cindy L. Von Haugg
M.S Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

The challenges associated with climbing trees to measure cavity dimensions have limited the accumulation of knowledge regarding wood duck (Aix sponsa) nesting habitat and ecology. To overcome this issue, we developed a 2-person method to measure external and internal tree-cavity dimensions from the ground. Our approach uses a telescopic pole, wireless cavity inspection camera with a monitor, and reference scale, allowing an object of known length to be viewed and recorded inside the cavity. We tested our method using simulated cavities (n = 20), assessed accuracy by comparing the estimated and actual measurements, and evaluated precision between 2 observers. The average difference (±1 SE) between estimated and actual measurements (n = 40) for entrance width (0.9 ± 0.9 cm), entrance height (0.8 ± 1.1 cm), platform width (0.1 ± 3.7 cm), and platform length (1.0 ± 3.2 cm) were ≤1 cm. There was no significant difference between observer measurements for entrance width, entrance height, platform width, or platform length. Observers overestimated cavity depth by an average of 0.1 ± 1.6 cm and there was a significant difference (1.3 ± 2.2 cm) between observers for mean cavity depth. We applied the technique to naturally occurring cavities. The time to complete a natural-cavity survey in the field (n = 37) averaged 12.2 ± 6.9 min. Our method increases the practicality, accessibility, and safety of researchers conducting cavity surveys for wood ducks and other cavity-dependent wildlife using a cost-effective, cavity-measuring tool.

Figure 1. The assembled wireless cavity monitoring system capable of measuring internal dimensions of wood duck nesting cavities using a reference scale that can be 1) stored; 2) attached to the camera; and 3) lowered into a cavity.

Figure 2. Two-person operation of a wireless cavity monitoring system capable of measuring external and internal dimensions of wood duck nesting cavities.

close up view of tree cavity

students measuring cavities in trees

Empowering Historic Rice Field Descendant Communities Through Community Gardens and Pollinator Education

Crystal Anderson
Doctoral Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Wetlands and the surrounding landscapes provide habitat for at-risk and endangered pollinator species such as the monarch butterfly (Danaus plexippus), southern plains pine bumble bee (Bombus fraternus), the rusty patch bumblebee (Bombus affinis), and a plethora of pollinators critical for ensuring sustainable gardens, agriculture, and ecological diversity in South Carolina. Among the 31 historical populations of monarch butterflies, 15 are facing the threat of extinction due to rising sea levels and excessively high temperatures caused by climate change. South Carolina is a critical stopover area for migrating monarchs, but a population of monarchs also overwinter in our area. The southern plains pine bumble bee, frequently found in the South Carolina coastal region, has experienced a significant loss in range, with a resulting 24% population decline since 2010. The rusty patch bumble bee has seen similar declines, especially with increased development and community HOA regulations that require frequent mowing during the critical emergence of this species. Community education and participation in pollinator gardens may increase pollinator abundance and diversity.

Hampton Plantation is a historical site located in the community of Germanville, South Carolina, inhabited primarily by descendants of those enslaved to work the historic rice fields. Many of the families surrounding Hampton Plantation have lived in this region for hundreds of years and continue to bury their family members within an active cemetery located on the property. The community surrounding Hampton Plantation faces food scarcity and high poverty rates due to its remote rural setting. In the US, 12.8% of households faced food insecurity in 2014, with higher rates among Black, Latino, and low-income families. Limited access to healthy food leads to health issues like depressive symptoms, diabetes, and heart disease. Food-insecure populations struggle to afford fresh food due to poor food environments. Alternative programs like community gardens may address these challenges, especially where traditional supermarkets are lacking. With nearly 75% of agricultural plants relying on pollinators to produce, understanding community needs and using pollinator plants and associated pollinators provides not only ecological benefit, but can play a pivotal role in empowering underserved communities to take charge of their dietary needs.

Social surveys will be conducted to establish a baseline understanding of historically preferred foods, as well as the economic and physical barriers to gardening and the ecological knowledge of Germanville community members concerning the benefits of pollinators. Pollinator gardens will be established at both Hampton Plantation and within the Germanville community for educational purposes. Additionally, multiple food gardens will be installed in the Germanville community to facilitate agricultural education and community empowerment. Throughout the three-year study period, community education events will be organized to promote gardening concepts and actively solicit feedback on any challenges to gardening progress, enabling project adjustments to enhance the likelihood of success. A final survey will be used in year three to determine the knowledge gained, the number of participants still active in the program. If the program is deemed a success, we will work with other SC State Parks to create additional community projects around historical rice fields.

bee pollinating a flower

crystal giving a presentation

The Social Influence: Antebellum Rice Field Restoration and Waterfowl Management

Crystal Anderson
Doctoral Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Historical antebellum rice fields in the low country of South Carolina have been deeply ingrained in the culture since the early 1700s. Through the installation of rice trunks, a large wooden apparatus that allowed the control of water levels through the rise and fall of tides, large-scale rice production was possible. Sprawling plantations brought immense prosperity to the southeast and ultimately changed our coastal geography. We understand today that tidal wetlands deliver a multitude of ecosystem services crucial for ecological well-being, as well as for the cultural and emotional well-being of residents. They offer services including the provisioning of food, fiber, fuel, and biochemical materials; climate regulation via hydrological flows, water purification, and erosion control; cultural protection and recreational opportunities; and essential ecological functions such as soil formation and nutrient cycling. Since the early 1900s, many of the 236,000 acres of antebellum rice fields across South Carolina’s coast have been left to nature, reverting to natural marsh. However, many are still actively managed for a different sort of production – waterfowl populations.

Antebellum rice fields provide a critical stopover wintering habitat for many waterfowl and waterbird species. The restoration and protection of antebellum rice fields may be an essential factor in slowing the onslaught of climate change and associated ecosystem service losses, as well as ensuring adequate waterfowl habitat for future populations. However, restoration can be cost-prohibitive, and with rising sea levels, we must assess at what point the restoration and protection of these historical relics are viable.

Management decisions that fail to consider the history, knowledge, and perceptions of the community can create resistance in community support, underscoring the need to understand human knowledge and perceptions to effectively address both environmental and social dimensions of resilience. Stakeholder surveys play an instrumental part in bridging the gap between policy and people. We are addressing the human dimensions of waterfowl management in antebellum rice fields by creating an in-depth survey that evaluates stakeholder knowledge of historic rice fields, cultural influence, and perceptions of associated infrastructure, and restoration. Out stakeholders are defined as state and private waterfowl managers, area residents, and culturally significant populations.

We anticipate that state and private waterfowl managers will have a strong knowledge of the ecological significance of waterfowl management. Area residents and culturally sensitive populations will have little understanding of ecological significance. State and private waterfowl managers and culturally significant stakeholders will have a strong desire to continue current rice field management strategies for waterfowl populations. However, we anticipate those with cultural ties to the area wishing to maintain traditional irrigation management through historical rice trunk technology. In contrast, state and private waterfowl managers and area residents may seek less cost-prohibitive methods for irrigation control.

Surveys will run from October 2024 through December 2024. The analysis will begin in January, with anticipated results by May 2025. These results will then be used in conjunction with aerial drone and acoustic surveys to help develop a decision support tool for future antebellum rice field management to promote coastal resiliency and future waterfowl populations.

birds in a rice field

map of historic rice fields in sc

Waterbird Habitat Selection in Georgetown County, South Carolina

Jordan McCall
M.S. Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Wetland habitats are ecologically valuable due to their high biological diversity and productivity, with many avian species depending on them. Understanding how waterfowl select habitats for use is essential to successful wetland management. The southeastern coastal plain of the United States contains 27% of the wetlands within the lower 48 states. However, only about 9% of these wetlands are of high or moderate value to waterfowl and other waterbirds. The region is comprised of 7 main wetland type classifications: (1) estuarine deepwater, (2) estuarine wetland, (3) freshwater emergent, (4) freshwater forested and shrub, (5) freshwater ponds, (6) lakes, and (7) riverine wetlands. Despite the significance of the Hobcaw Barony in a region historically known as a waterfowl hunting paradise, the long history of the Belle W. Baruch Institute of Coastal Ecology and Forest Science on Hobcaw Barony and the establishment of the James C. Kennedy Waterfowl and Wetlands Conservation Center at the Baruch Institute in 2014, no comprehensive study of waterbirds has occurred on site. Thus, this study aims to form a fundamental baseline of wetland and waterbird data as the beginning of a long-term dataset. Our specific objectives were to 1) determine which wetland type is hosting the most waterbirds in terms of species diversity and abundance and 2) determine what environmental factor(s) are contributing to the most productive wetland type at Hobcaw Barony and DeBordieu Colony.

This study along the South Carolina coast began in January of 2022, encompassing extensive, conserved lands and highly developed and altered landscapes. Two field seasons have since been completed from February-July of 2022 and January-July of 2023. Research sites were at the Hobcaw Barony (~7,000 ha) and the DeBordieu Colony (~1,000 ha).

In 2022, we performed point count surveys at 95 randomly selected wetlands, varying by type, and secretive marshbird surveys at 10 emergent wetlands to estimate occupancy rates, species diversity, species abundance, and migration chronology. In 2023, we performed point counts in 98 randomly selected wetlands and secretive marshbird surveys in 8 emergent wetlands. In year 2, we readjusted and maximized sampling effort on wetland types where waterbirds were most abundant and decreased on the remaining types (based on the availability of time to survey) where few birds were observed, surveying in 98 wetlands. Wetland-level data (e.g., water quality, water regimes, vegetation, macroinvertebrates) were also collected to model waterbird use and selection of wetlands. To determine which environmental factor(s) were driving waterbird attraction, we will perform a generalized linear mixed model with a Poisson distribution for each species guild and our top 12 detected species.

We detected 4,099 waterbirds over our two field seasons. We observed 56 species, with 34 of those species seen in both field seasons, 8 species unique to 2022 and 13 species unique to 2023. We observed 1,518 wading birds (10 species), 744 shorebirds (16 species), 844 waterfowl (13 species), 347 marshbirds (6 species), 161 gulls-terns (6 species), 445 anhingas-cormorants-pelicans (4 species), and 40 grebes (1 species). Preliminary results from the generalized linear model for waterfowl reveal that the following environmental factors have a significant effect on their presence: location, wetland classification, vegetation distribution pattern, water regime, wind direction, distance to nearest wetland, wetland area, macroinvertebrate species richness, and percent vegetation cover. Waterfowl had a significant positive relationship with DeBordieu, specifically, lacustrine limnetic unconsolidated bottom wetlands (i.e., lakes) and permanently flooded open-water wetlands. These results are no surprise as DeBordieu contains primarily this wetland type, and historically, this wetland type highly supports waterfowl species.

This study would not have been possible without funding support from the DeBordieu Colony and the James C. Kennedy Waterfowl and Wetlands Conservation Center, and logistical support from the Nemours Wildlife Foundation, Belle W. Baruch Foundation, and Clemson’s Department of Forestry and Environmental Conservation. We also appreciate Jack Corbin, Anna Koon, Carly Sprott, and Blair Abernathy for their assistance in the field.

map of a waterbird habitats in SC

goes through a fisheye lens

Waterbird Spring Migration Chronology in Georgetown County, South Carolina

Jordan McCall
M.S. Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

The South Coastal Plain of South Carolina is essential for migrating, wintering, and breeding waterfowl and other waterbirds. Historically, 30 to 50% of Atlantic Flyway green-winged teal (Anas crecca), northern shoveler (Spatula clypeatea), mallard (A. platyrhynchos), northern pintail (A. acuta), American wigeon (Mareca americana), and gadwall (M. strepera) wintered in the region. However, recently, waterfowl have been wintering further north, possibly due to climate change and warming temperatures. Waterfowl often migrate due to changes in habitat conditions, resource availability, nesting location, breeding season, and other factors. Spring migration for waterfowl, specifically, is an essential phase of their annual cycle and can provide critical information when it comes to wetland management. Despite the significance of the Hobcaw Barony in a region historically known as a waterfowl hunting paradise, the long history of the Belle W. Baruch Institute of Coastal Ecology and Forest Science on Hobcaw Barony and the establishment of the James C. Kennedy Waterfowl and Wetlands Conservation Center at the Baruch Institute in 2014, no comprehensive study of waterfowl or waterbirds has occurred on site. Thus, this study aims to form a fundamental baseline of wetland and waterbird data as the beginning of a long-term dataset. Our primary objectives were to 1) document winter and spring temporal trends and spring migration chronology of waterbirds using wetlands on Hobcaw Barony and DeBordieu Colony (an adjacent development) and 2) determine which species have similar migration patterns.

This study along the South Carolina coast began in January of 2022, encompassing extensive, conserved lands and highly developed and altered landscapes. Two field seasons have since been completed from February-July of 2022 and January-July of 2023. Research sites were at the Hobcaw Barony (~7,000 ha) and the DeBordieu Colony (~1,000 ha).

In 2022, we performed point count surveys at 95 randomly selected wetlands, varying by type, and secretive marshbird surveys at 10 emergent wetlands to estimate occupancy rates, species diversity, species abundance, and migration chronology. In 2023, we performed point counts in 98 randomly selected wetlands and secretive marshbird surveys in 8 emergent wetlands. In year 2, we readjusted and maximized sampling effort on wetland types where waterbirds were most abundant and decreased on the remaining types (based on the availability of time to survey) where few birds were observed. To document temporal trends, we averaged species densities in each wetland and graphed them over two-week periods. We also performed a complete linkage hierarchical cluster analysis to identify which species had similar migration patterns.

We detected 4,099 waterbirds over our two field seasons. We observed 56 species, with 34 of those species seen in both field seasons, 8 species unique to 2022 and 13 species unique to 2023. We observed 1,518 wading birds (10 species), 744 shorebirds (16 species), 844 waterfowl (13 species), 347 marshbirds (6 species), 161 gulls-terns (6 species), 445 anhingas-cormorants-pelicans (4 species), and 40 grebes (1 species). On average, wading birds peaked in late March at 2.92 ± 1.09 birds/ha; shorebirds peaked in mid-May at 3.37 ± 1.35 birds/ha; waterfowl peaked in late March at 1.91 ± 1.42 birds/ha; secretive marshbirds peaked in late April at 0.83 ± 0.29 birds/ha; pelicans, anhingas, and cormorants peaked in late March at 0.89 ± 0.31 birds/ha, as well as gulls and terns at 0.43 ± 0.27 birds/ha. The cluster analysis revealed two large species clusters, cluster A, more frequently occurring from April to July, and cluster B, more regularly occurring from January to March, including almost all waterfowl species.

Of the 56 species detected, 14.3% occurred throughout our survey period, with most being herons, egrets, and anhingas. The remaining 85.7% exhibited patterns of increasing or decreasing in the study area due to rainfall, tide levels, and simply using the property as a stopover site during migration. Our study identified a very low overall density for most species, which could have been due to weather during our field seasons, such as a lack of rainfall or overall low habitat quality. When Hurricane Hugo hit these properties in 1989, it caused a decline in the total number of vegetation species present at Hobcaw, with Phragmites rapidly colonizing these areas. Further vegetation declines occurred between 2013 and 2015, likely due to rising sea levels and an increase in water salinity, possibly contributing to the low species densities. We recommend that future managers of these and surrounding properties use these data to know when and how to manipulate their wetlands. Future research could include performing point-count surveys during low or high tide only to standardize conditions, using drone technology to increase counts further than just the eye can see, and collecting macroinvertebrate data more often (e.g., monthly) to analyze how their population densities align with waterfowl.

This study would not have been possible without funding support from the DeBordieu Colony and the James C. Kennedy Waterfowl and Wetlands Conservation Center, and logistical support from the Nemours Wildlife Foundation, Belle W. Baruch Foundation, and Clemson’s Department of Forestry and Environmental Conservation. We also appreciate Jack Corbin, Anna Koon, Carly Sprott, and Blair Abernathy for their assistance in the field.  

researcher collecting measurements near a marsh

a collection of waterbirds in a lake

Development of a Decision Support Tool for Managing Antebellum Rice Fields in South Carolina, United States of America

Oluwatobi Emmanuel OLANIYI
Doctoral Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Antebellum rice fields along South Carolina's coast, once key for rice farming, today provide critical habitat for bird species like warblers, shorebirds, and waterfowl. Planters used to maintain these fields for economic advantage, but they are now prized for their more comprehensive environmental benefits. With increasing sea levels threatening these places, there is an urgent need for novel management solutions that fulfill both ecological preservation and community needs. This study aims to develop a decision-support tool for managing these historic rice fields. It will involve collecting and integrating data on environmental and social aspects, assessing restoration methods, and creating a risk-benefit matrix profile to evaluate the impacts of various management actions.

This research uses a mixed-methods strategy that combines quantitative and qualitative methodologies to assess the restoration of antebellum rice fields. It uses geospatial analysis, field assessments, and socioeconomic evaluations to understand restoration results comprehensively. The Analytical Hierarchy Process (AHP) is used in the study to make multi-criteria decisions, which will aid in assessing numerous success variables and prioritizing restoration activities. The study engages a diverse group of stakeholders, including Gulla Geechee, the public, and plantation managers, focusing on ten restoration sites across key river basins. Data collection methods include GIS and remote sensing for tracking land use and cover changes, field-based ecological assessments, and socioeconomic surveys through questionnaires and focus group discussions. For data analysis, AHP will assist in evaluating and prioritizing success criteria, while geospatial analysis, machine learning models, generalized linear modeling, Bayesian Belief Networks, and Python libraries will be employed to conduct scenario analysis and develop risk-benefit matrix profiles and decision support tools.

The findings are expected to reveal the effectiveness of restoration efforts, focusing on key indicators such as ecological dominance, diversity, hydrological connectivity, and socioeconomic impacts. The study will also provide insights into the risks and benefits associated with ecological and societal factors. Also, the development of a decision support tool will facilitate informed management decisions by integrating various data and analysis scenarios. Results will be summarized in tables showing restoration success and socioeconomic impacts, and figures illustrating risk-benefit analyses and the decision support tool's interface.

A presentation about ecology

graph explaining the concepts of socioecological systems

Baseline Ecological Data of a Shrimp Farm Slated for Restoration in Coastal South Carolina 

Rene Brown
M.S Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservations Center

One of the most prevalent substances on Earth is water, which is made up of hydrogen and oxygen and is essential to all living things. It is the main component of the majority of living things, including people. Water availability is a major limiting factor for plant productivity in terrestrial ecosystems globally, even though it is abundant on Earth's surface. This emphasizes how vital water is to the survival of ecosystems in general and wetlands in particular.

Wetlands are ecosystems distinguished by soil, vegetation, and hydrological characteristics. These include certain soil types, plants that do well in damp environments, and the existence of standing water for a portion of the growth season. Another noteworthy quality of wetlands is their capacity to filter metals out of surface and groundwater, improving the quality of the environment. These characteristics highlight their significance as natural filtration systems that preserve water quality as well as biodiversity hotspots.

The Eastern oyster, or Crassostrea virginica, is a keystone species that offers a multitude of ecological functions and is one of the species that are essential to wetland and estuary environments. As a commercial product, oysters support coastal economies and provide food and building materials. Oysters have a significant ecological impact in addition to being economically valuable since they filter water, enhance its quality, and provide habitat for other species. Particularly in regions where anthropogenic activities have an influence, their existence increases the resilience of the ecosystem.

Plant litter decomposition in estuarine wetlands is crucial for short-term carbon storage, nutrient cycling, and coastal trophodynamics. Aquatic ecosystems depend on the decomposition of organic matter to recycle nutrients and other chemical elements, maintain vital food chains, and support primary production. Although the coastal ecosystem of Little Edisto Island is still mostly intact, problems with water quality could endanger the oyster habitats, plant communities, and fish populations. One method for developing living shorelines and stabilizing coastal systems is the restoration of native oyster populations. Oysters are essential to these habitats because they grow in unique formations like "oyster flats" along creeks and rivers or on the edges of marshes. The former shrimp farm site S-161 on Little Edisto Island offers a chance for oyster farming and wetland restoration. Impoundments will be converted into oyster beds, new buildings will be erected, and water filtration will be improved to promote oyster development at this site.

The goal of this study is to clarify the relationships between environmental variables and the condition of these essential ecosystems. The research will improve conservation and restoration methods targeted at strengthening the resilience and sustainability of these ecosystems in the face of anthropogenic and climatic stressors by gaining an understanding of the factors that affect oyster and plant populations.

The breakdown of plant litter, an essential process for the nutrient cycle and overall health of salt marsh ecosystems, is a major area of attention for this research. In an impounded salt marsh, the decomposition rates of leaf litter from Juncus roemerianus and Sporobolus virginicus are investigated in this study. The study quantifies the decomposition process and determines the environmental conditions causing these changes by tracking mass loss over time using litter bags. When compared to natural marshes, the peculiarities of impounded marshes may change the dynamics of decomposition, affecting the availability of nutrients and the general health of the ecosystem. These results will help create better management strategies to preserve the ecological balance and productivity of reclaimed salt marshes. By integrating these different aspects of wetland ecosystems, the research aims to provide insights into ecosystem functioning and restoration, contributing to the broader goals of wetland conservation

Rene near a lake collecting data

Rene and another student looking through binoculars

Developing Anuran, Avian, Macroinvertebrate, and Vegetative Indices of Biotic Integrity for Isolated Carolina Bays

Scott Binger
Doctoral Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Wetlands are highly impactful ecosystems, providing ecosystem services including water quality and flooding regulation, carbon sequestration, and harvestable resource provisioning (Mitsch et al. 2015). However, wetlands are also some of the least protected ecosystems in many areas (Barbier 2011). Additionally, wetlands that are isolated (lacking direct connections to rivers, streams, estuaries, or the ocean) face increased vulnerability to loss and extensive disturbance (McCauley et al. 2013). Carolina Bays are unique isolated wetlands found throughout coastal South Carolina that provide important habitat for rare species but have faced extensive loss due to urbanization and agriculture (Sharitz 2003). Conservation efforts in these areas require the ability to quickly assess the biotic and abiotic conditions of these sites, and the ability to gauge how these conditions respond to human disturbance and environmental change. Indices of Biotic Integrity (hereafter referred to as IBIs) are useful tools that allow these assessments to be made using data on biological communities (Veselka and Anderson 2013). Anuran, avian, macroinvertebrate, and vegetative community compositions each respond to changes in conditions differently, and data on these compositions can be used to derive a variety of metrics that can be included in IBIs, allowing for indices that capture a range of important biotic responses to disturbance that can be easily applied across sites and used to inform wetland management decisions. This study seeks to create IBIs for Carolina Bay wetlands, with the goal of assessing how their biotic communities respond to human disturbance and creating actionable, easily interpreted research tools to assist management efforts.

This study will use a mixed-method approach, sampling 60 Carolina Bay wetlands over 2 years from 13 counties in South Carolina. For the biotic data in each wetland, we will quantify the abundance and biomass of macroinvertebrates by family or finer resolution, collect quantitative and qualitative measures of plant species presence and cover, determine relative abundances of anuran species via call surveys, and determine the abundance of avian species via point count surveys. These sampling efforts will occur seasonally throughout the year, repeated as necessary. We will develop a disturbance gradient based on land use, hydrological alteration, and habitat alteration/development. This gradient will be used to score each site. In addition, we will measure soil and water chemistry and hydroperiod to determine abiotic variables influencing community composition. Sites will be divided into reference and stressed sites by degree of disturbance, and we will conduct comparisons of potential IBI metrics between reference and stressed sites. We will test and grade metrics by the level of overlap in the interquartile ranges of values for reference and stressed sites, and the highest-graded metrics for each taxon will be kept. We will use generalized linear models to evaluate the response of these metrics to human impairment, which will inform which metrics are combined to create IBIs for each taxonomic group and cumulatively across taxonomic groups.

We anticipate that the best performing IBIs, with regard to their response to human impairment, will use combinations of metrics that respond to a wide range of stressors, and that these IBIs will help to identify 1). high-risk taxonomic groups 2). sites of high vulnerability and 3). highly impactful stressors. We hope that, by identifying these, we can create tools that will streamline the assessment of sites and improve potential management outcomes in isolated wetlands.

References

A collection of Venus flytrap plants

A collection of trees in a field

Mapping Climate Vulnerability Risk: PFAS and Microplastics in the South Carolina Coastal Plain

Dorothy Aldridge
Master’s Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Introduction

Coastal wetlands serve as transitional systems that filter contaminated stormwater runoff and industrial discharges, often sequestering and transforming harmful pollutants through microbial processes and plant uptake. However, contaminants of emerging concern that resist degradation, such as per- and polyfluoroalkyl substances (PFAS) and microplastics, persist and accumulate in wetland sediments, threatening wildlife and fisheries. Despite heightened climate influence on these low-elevation systems, research is limited to the integrated effect of climate and landscape changes on the distribution of these contaminants. The objective of this study is to synthesize meteorological and environmental drivers of PFAS and microplastic presence in South Carolina’s coastal plain, together with indicators of resilience, to map watershed vulnerability. The resulting risk assessment will reveal which coastal watersheds are most susceptible to contaminant exposure, and how that vulnerability varies under multiple greenhouse gas emission scenarios.

Methodology

We will compile publicly available PFAS and microplastic data from the South Carolina Department of Environmental Services’ (SCDES) Ambient Surface Water PFAS Monitoring project, open access data repositories, and collected surface water samples. To illustrate current contamination levels, we will generate predictive hotspot maps for each contaminant using the GIS Pro Universal Kriging toolkit to produce rasterized surface water concentration values within the South Carolina coastal plain and nearshore marine waters. We will calculate vulnerability scores for each Hydrologic Unit Code (HUC10) watershed (~200 mi²) using the Intergovernmental Panel on Climate Change (IPCC) framework, which defines vulnerability as a function of exposure, sensitivity, and adaptive capacity (IPCC 2001; IPCC 2007). The project team will select quantitative exposure indicators within categories of hydrology, precipitation, and sea level rise. To model meteorological variables under two greenhouse gas emission scenarios, Representative Concentration Pathways (RCP) 4.5 and 8.5, we will use the Hydrologic and Water Quality System (HAWQSv2.0) online tool, which statistically downscales multiple global climate models. The analysis aggregates projected changes from the historical baseline (1986–2005) to the midcentury future (2040–2059) into composite exposure scores. These values are compared to quantitative measures of a watershed's capacity to adapt to contaminant exposure such as landcover change, wetland type, stormwater pond density, and percent impervious surfaces. The project team will weigh these indicators of resilience and exposure and synthesize them into a vulnerability calculation.

Anticipated Results

We expect indicators of increased anthropogenic influence, measured by population density, land use alteration, and percent impervious surfaces, to have a positive relationship with vulnerability scores. Environmental characteristics such as high soil organic matter and low energy hydrodynamics are also expected to increase PFAS exposure scores. We expect to see greater vulnerability scores under the higher RCP 8.5 emissions scenario compared to RCP 4.5, due to higher sea levels inundating novel contaminant sources and increased deposition of pollutant runoff into low-lying wetlands. However, we predict the difference between emission scenarios will be lower in watersheds implementing climate mitigation infrastructure, such as stormwater ponds, living shorelines, and seawalls.

Discussion

By integrating complex climate and contaminant variables, this approach provides land managers and decision makers with actionable information that is otherwise difficult to synthesize. Evaluating vulnerability under multiple emission scenarios captures the dynamic nature of coastal systems and supports adaptive management strategies that incorporate future climatic conditions. Comparing species’ life histories with the location and drivers of contaminant hotspots in South Carolina’s coastal plain can help to distinguish species most at risk of exposure such as long-lived waterfowl reliant on aquatic food sources.

Acknowledgments

I acknowledge the support of Miriam Boucher, Dr. Stefanie Whitmire, Dr. Thomas Rainwater, and Dr. Jim Anderson, as well as South Carolina Sea Grant, for providing funding for this research (NOAA award No. NA24OARX417C0591 – CFDA#11.417).

References

  • Intergovernmental Panel on Climate Change. (2001). Climate change 2001: Impacts, adaptation, and vulnerability (J. J. McCarthy, O. F. Canziani, N. A. Leary, D. J. Dokken, & K. S. White, Eds.). Cambridge University Press.
  • Intergovernmental Panel on Climate Change. (2007). Climate change 2007: Impacts, adaptation and vulnerability (M. L. Parry, O. F. Canziani, J. P. Palutikof, P. J. van der Linden, & C. E. Hanson, Eds.). Cambridge University Press.

Dorothy on a boat

Dorothy collecting samples

a lakeside

ducks in lake

Investigating the presence and health implications of four PFAS in wood duck (Aix sponsa) populations across South Carolina

Jake Shurba
Doctoral Student, Clemson University
James C. Kennedy Waterfowl and Wetlands Conservation Center

Introduction

Per- and polyfluoroalkyl substances (PFAS), or “forever chemicals,” are synthetic compounds
used in industrial and commercial products for their resistance to heat, water, and oil. Their
chemical stability has led to environmental persistence, especially in aquatic systems, where they
accumulate in water, soil, and wildlife. In South Carolina, PFAS has been detected in over 100
water bodies, with notable concentrations near military installations that use aqueous film-
forming foams. Our research aims to determine how widespread PFAS are in coastal wetlands
near military and non-military lands in South Carolina, and what the potential health and
ecological risks associated with PFAS accumulation in waterfowl.

Objectives:

  1. Establish a baseline dataset of four PFAS (PFOS, PFOA, PFHxS, PFHxA) in soil and
    water.
  2. Assess PFAS exposure in wood ducks by age and sex.
  3. Quantify PFAS in plasma, feces, and muscle tissue.
  4. Investigate links between PFAS and duck health indicators.
  5. Determine maternal transfer via egg albumin.
  6. Evaluate potential human health risks from waterfowl consumption.

Methodology:

We will use quantitative, field-based contaminant monitoring and physiological health
assessment. Samples will be collected from six coastal sites (military, state, federal, and private
lands) and from wood ducks captured via nest boxes (Figure 1), live-trapping, and hunter harvest
(Figure 2).

Data Collection:

  • Environmental: Soil (~100 g) and water (500 mL) samples collected at five random points per site (30 total samples/year for each medium).
  • Waterfowl:
    • Live capture: Blood, feces, and morphometrics collected; individuals banded.
    • Hunter-harvested: Breast muscle biopsy, blood, feces collected.
    • Reproduction: Two eggs per nest collected for PFAS in albumin (Figure 3).

PFAS concentrations will be analyzed in all samples. Blood will be evaluated for packed cell
volume (PCV), total protein (TP), and immune response via red/white blood cell smears. Plasma
will be separated via centrifugation. Data will be analyzed using standard contaminants and
hematological assessment protocols.

Anticipated Results:

  • PFAS are expected to be present in all sample types, with higher concentrations near military sites.
  • Wood ducks are likely accumulating PFAS in tissues, plasma, feces, and potentially transferring them to eggs.
  • Differences in PFAS levels are anticipated across age and sex classes.
  • Health assessments (PCV/TP and blood smears) may show correlations between PFAS burden and immune or chronic stress indicators.
  • Muscle tissue concentrations will inform human exposure risk through consumption.

Interpretation:

This study will provide the first comprehensive look at PFAS contamination in wood ducks and
their coastal habitats in South Carolina. It addresses critical data gaps related to environmental
exposure, reproductive transfer, and health risks for both wildlife and humans. The findings will
inform land managers, public health officials, and the hunting community about potential risks
associated with PFAS in wetland ecosystems and waterfowl.

Dorothy on a boat

Dorothy collecting samples

a lakeside

ducks in lake

Comparative Genetics and Movement Patterns of Wild and Game-Farm Mallards in the Southeastern United States

James C. Kennedy Waterfowl and Wetlands Conservation Center

Project Overview

Mallards (Anas platyrhynchos) are one of the most widely distributed ducks in the world, long
valued by people for food, hunting, and conservation. In North America, their numbers rose
sharply in the 20th century, and they now rank among the most harvested ducks in the Atlantic
Flyway. Part of this increase stemmed from large-scale releases of captive-raised mallards, with
hundreds of thousands released annually along the eastern seaboard during the last century.
While these introductions bolstered hunting opportunities, they also came with trade-offs:
interbreeding between farm-raised and wild mallards has blurred genetic distinctions, potentially
reducing the adaptability of wild populations and threatening their long-term resilience.

In response, the James C. Kennedy Waterfowl and Wetlands Conservation Center at Clemson
University, working with the South Carolina Waterfowl Association, Palmetto Waterfowl, and
the University of Texas at El Paso, launched a multi-year research effort to investigate how
released mallards interact with their wild counterparts. This work combines genetic testing,
movement ecology, and predator-prey observations to answer a fundamental conservation
question: what are the ecological and genetic consequences of mixing farm-raised and wild
mallards in South Carolina?

Progress to Date

Fieldwork began in summer 2025 with banding of farm-raised mallards. Every bird received a
USGS leg band, establishing long-term tracking through national recovery networks.

This fall, the project is expanding into several new phases:

  • Nanotags: Lightweight tags will allow researchers to detect the presence or absence of
    marked birds at release sites through automated receiver networks.
  • GPS-GSM Transmitters: Twenty-five mallards will carry satellite transmitters that record
    and relay fine-scale movements, allowing the team to examine flight distances, dispersal,
    and habitat use across the flyway.
  • Genetic Analysis: All birds handled in fall will be bled to compare genetic signatures of
    wild, farm-raised, and hybrid individuals, providing baseline measures of genetic
    integrity.

Next Steps

In spring 2026, trapping will focus on wild mallards to expand the dataset. Captured birds will be
banded, sampled for DNA, and fitted with GPS transmitters to track seasonal and long-distance
movements. Blood samples collected across refuges and hunt club properties will be sequenced
in partnership with the Lavretsky Lab at UTEP, offering high-resolution insight into the
proportion of pure versus hybrid lineages.

Anticipated Outcomes

This study is expected to highlight key contrasts among mallard groups:

  • Movement Ecology: Captive-origin birds may show shorter, less consistent movement patterns compared to wild birds.
  • Genetics: Farm-raised mallards are predicted to carry lower genetic diversity, a warning sign for adaptability.

Data will be analyzed using a combination of statistical approaches (e.g., ANOVA, regression) to
compare group differences in behavior and movement, and nesting success.

Broader Impact

The implications extend far beyond South Carolina. If released mallards consistently dilute the
genetic integrity of wild populations or perform poorly in the wild, conservation strategies may
need to adapt, potentially incorporating breeding programs that restore pure North American
mallard lineages. By linking genetic studies with ecological data, this research will provide
managers with the knowledge needed to safeguard healthy and resilient waterfowl populations in
the Atlantic Flyway.

tracking leg band

funnel trap

mallard in lake

motus tower