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Creative Inquiry + Undergraduate Research

Improving Embryos in vitro

Improving Embryos in vitro

In recent years, there have been notable advancements in the field of in vitro fertilization (IVF) and related fertility procedures. In vitro fertilization is the process by which an egg is artificially fertilized in a lab, and then reimplanted into the mother’s uterus to increase the chances of a pregnancy. Many of these advances are the result of research that started with animal embryos. The In vitro Fertilization and Embryo Culture Creative Inquiry project, mentored by Drs. Celina Checura and Matthew Hersom from the Piedmont Research and Education Center, is working to contribute to this fast-advancing field.

Carolina Elgaressta, Logan Thomason, Hannah Culler, Christian Wilhelm, Katie Kirkman, Izabella Zaraca and Dr. Celina Checura pose with their award plaque
 The CI team signed their award plaque from the International Embryo Technology Society Conference
The CI team signed their award plaque from the International Embryo Technology Society Conference

This Creative Inquiry team, works with bovine embryos to enhance IVF procedures. First they investigated the effects of different growth media on developmental rates of the embryos. They also assessed the impact of photo biomodulation, the exposure to different wavelengths of light, on developmental rate. For this procedure the students exposed the embryos to LED red light for ten minutes at 21 hours post insemination. The effect on developmental rate was determined by looking at the blastocyst rates—the appearance of dividing cell clusters that lead to a pregnancy—in the embryos exposed to the light treatment. The team found that blastocyst rate was not affected by photo biomodulation.

The Creative Inquiry team presented their research at the Annual International Embryo Technology Society Conference in Denver were they were runner up in the undergraduate student poster competition. They also attended presentations and networking events with industry professionals—invaluable opportunities that will benefit their future careers and educational pursuits.

“Gaining all the experience through this lab will definitely give me a leg up in the master’s program,” Carolina Elgaressta, a junior genetics major, said.

Looking ahead, the team plans to repeat their previous photo biomodulation experiments at different time intervals after insemination. They hope to determine if the effects of the light will enhance the embryo development at specific times. This research holds potential for significant impacts on in vitro fertilization in bovines, and may pave the way for breakthroughs in human reproductive technologies.

Carolina Elgaressta

“Gaining all the experience through this lab will definitely give me a leg up in the master’s program.”

Carolina Elgaressta
Genetics

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Creative Inquiry + Undergraduate Research
Creative Inquiry + Undergraduate Research | Watt Family Innovation Center 405 S. Palmetto Blvd, Suite 303 Clemson, SC 29634-5733