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Biological Sciences Profiles

Wilbur (Wil) Milhous

Lecturer in Microbiology and Infectious Disease
Biological Sciences Department

Office: G19 Jordon
Phone: 813-778-3439


 Educational Background

PhD Public Health Microbiology & Parasitology
UNC Chapel Hill 1983

MS College of Agr & BioSci
Clemson 1972

BS Biological Sciences
Clemson 1970

 Courses Taught

Teaching Experience at Clemson
BIOL 4560 - Medical and Veterinary Parasitology Fall 2017-18
MICR 4560 - Medical and Veterinary Parasitology Fall 2017-18
BIOL 6560 - Medical and Veterinary Parasitology Fall 2017-18
MICR 6560 - Medical and Veterinary Parasitology Fall 2017-18
MICR 4110 - Pathogenic Bacteriology (HON) Spring 2018-19
MICR 4120 - Bacterial Physiology Spring 2017
MICR 6110 - Pathogenic Bacteriology Spring 2017-18
MICR 6120 - Bacterial Physiology Spring 2017

Teaching Experience at University of South Florida as the Associate Dean for Research, College of Public Health and Director of the Global Health Infectious Disease Research Program (GHID)
HSC4631 Undergraduate: Critical Issues in Public Health Fall 2013 Spring 2014
PHC 7931 Ethical Decision Making in Public Health Research, Fall 2015
PHC 7931 and CHM Drug Discovery for Tropical Diseases Spring Semester 2014-2016
PHC7931 Advanced Interdisciplinary Seminar in Public Health Summer 2010, 2011, 2012
PHC7931 Global Health Infectious Diseases Seminar Fall 2007-2017
PHC6510 Global Emerging Infectious Diseases Fall 2008-2017
PHC6945 Global Health Field Experience Fall 2008-2017
PHC6115 Global Health Principles & Contemporary Issues Fall 2008-2017
PHC7931 Global Health Drug Discovery for Tropical Diseases Spring 2009-201)
PHC6934 Infectious Disease Epidemiology-Vaccines (Summer 2009)
CHE6936 Medicinal Chemistry Colloquium (Spring 2009-2017)

Previous Teaching Experience at the Walter Reed Army Institute of Research (WRAIR)
2007-2008 Rollins School of Public Health, Emory University, Atlanta, Georgia
GH 590R- Malaria: A Global Public Health Concern

1985-2007 WRAIR Formal accredited training programs
Preventive Medicine Residency
Laboratory Animal Medicine Residency
Clinical Pharmacology Fellowship
1985-2007 WRAIR Postdoctoral programs
Medical Research Fellowship
National Research Council Post-Doctoral Associateships
1985-2007 WRAIR Community Outreach Programs
Gains in the Education of Mathematics and Science, ages 13-18
Science and Engineering Apprenticeship Program-SEAP for high school students
College Student Leadership Program-CQL for college students

1990-2007 Adjunct Associate Professor (Assistant 1984-1990) Uniformed Services University of the Health Sciences, Bethesda, MD.
Medical Microbiology/Parasitology for Medical Students (16 semesters)
Chemotherapy/Pharmacology for MPH/PhD Students (5 semesters)
Scientific Mentor, Clinical Pharmacology Fellowship Training Program


College of Biological Sciences
G19 Jordon Hall Email:
Professor Wil Milhous is currently a Lecturer in Infectious Disease Microbiology & Parasitology and comes to Clemson as subject matter expert in the field of Global Health and Antimicrobial Chemotherapy. He received his PhD in 1983 from the UNC Gillings School of Global Health in a combined training with industry program at Burroughs Wellcome (now GSK) and has over thirty years of drug development experience with a primary focus in malaria and emerging diseases. After UNC, Wil joined the Walter Reed Army Institute of Research (WRAIR) working in Experimental Therapeutics for discovery, lead optimization, translational research and clinical trials supporting the Military Infectious Disease Research Program with FDA approval of Intravenous Artesunate, Malarone, Arakoda and Krintafel. Wil obtained specialized infectious disease training from NIH, CDC and Duke and served as the Consultant to the Army Surgeon General in Medical Microbiology. He is a Professor Emeritus at WRAIR, which the oldest school of Public Health and Preventive Medicine in the Nation. In 2007, Wil joined the faculty of the USF GHIDR program and became a member of the Medicines for Malaria Expert Scientific Advisory Committee in Geneva. He was founding co-chair of the Committee on Global Health of the American Society of Tropical Medicine and Hygiene (ASTMH). Milhous has been a member of the American Society of Microbiology (ASM) for over forty years and is a Fellow of the American Academy of Microbiology and Diplomate in Medical and Public Health of the American Board of Medical Microbiology. Milhous was the former Associate Dean for Research and Director of the Center Global Health Infectious Disease Research (GHIDR), College of Public Health, University of South Florida, Tampa, Florida, where he served for the ten years. The College was ranked 16th nationally and 2nd in the South. Prof Milhous had active affiliations and leadership roles in managing the College’s diverse multimillion ($50M in 2016/17) research portfolio. He held a joint appointment with the College of Medicine, Department of Internal Medicine. Milhous has received numerous honors and awards to include the ASTMH-LePrince Medal in Malariology, the Gorgas Medal in Preventive Medicine, ASTMH-Ashford Medal, Distinguished Alumnus of UNC, USF Research Achievement Award as well as numerous military awards during 30 years of active federal service. Milhous has over 170 publications with over 11,000 citations including 14 books/book chapters and 10 therapeutic patents. Milhous taught medical parasitology to 1st year medical students at the Uniformed Service University in Bethesda, Maryland, while assigned to the WRAIR with frequent short and long-term deployments to Military Infectious Disease overseas labs in Brazil, Peru, Thailand, Kenya, Ghana, Australia and Indonesia for drug and vaccine development. Colonel (ret) Milhous is a native of South Carolina with a BS/MS and ROTC commission from Clemson.

 Research Interests

Drug Discovery and Development for Neglected Diseases with special emphasis in Malaria
Drug Target Identification, Validation, Lead Optimizations, Candidate selection, Advanced Pre-Clinical, Clinical Phase 1,2,3 and Postmarketing Surveillance
Global Health Infectious Disease, Health Disparities


1. Obaldia N, Milhous WK, Kyle DE. 2018. Reversal of chloroquine resistance of Plasmodium vivax in Aotus monkeys. Antimicrob Agents Chemother. Aug 27;62(9). pii: e00582-18. doi: 10.1128/AAC.00582-18.
2. Gargano LM, Engel J, Gray GC, Howell K, Jones TF, Milhous WK, Morris JG, Mead, DG, Rubin C, Unnasch TR, Woods CW and Hughes JM. 2013. Arbovirus Diseases. Southeastern United States. Emerg Infect Dis Volume 19, Number 11—November 2013 [Internet].
3. Serebrennikova YM, Patel J, Milhous WK, Garcia-Rubio LH, Huffman DE, Smith JM.
Spectrophotometric detection of susceptibility to anti-malarial drugs. Malar J.
2013 Aug 30;12:305. doi: 10.1186/1475-2875-12-305. PubMed PMID: 23992478; PubMed
Central PMCID: PMC3849014.
4. Perić M, Fajdetić A, Rupčić R, Alihodžić S, Ziher D, Bukvić Krajačić M, Smith
KS, Ivezić-Schönfeld Z, Padovan J, Landek G, Jelić D, Hutinec A, Mesić M, Ager A,
Ellis WY, Milhous WK, Ohrt C, Spaventi R. Antimalarial activity of 9a-N
substituted 15-membered azalides with improved in vitro and in vivo activity over
azithromycin. J Med Chem. 2012 Feb 9;55(3):1389-401. Epub 2012 Jan 25. PubMed
PMID: 22148880.
5. Gargano LM, Gallagher PF, Freeman AS, Morris JG Jr, Temporado Cookson S, Greenwald I, Milhous WK, Williams-Johnson M, Wolfe C, Isakov A, Woods CW and Hughes, JM Academic consortia: untapped resources for preparedness, response, and recovery—examining the cholera outbreak in Haiti. Emerg Infect Dis [serial on the Internet] Vol 17 No. 11. 2011
6. Dow GS, Gettayacamin M, Hansukjariya P, Imerbsin R, Komcharoen S, Sattabongkot
J, Kyle D, Milhous W, Cozens S, Kenworthy D, Miller A, Veazey J, Ohrt C. Radical
curative efficacy of tafenoquine combination regimens in Plasmodium
cynomolgi-infected Rhesus monkeys (Macaca mulatta). Malar J. 2011 Jul 29;10:212.
PubMed PMID: 21801400; PubMed Central PMCID: PMC3161915.
7. Bukvić Krajačić M, Perić M, Smith KS, Schönfeld ZI, Žiher D, Fajdetić A,
Kujundžić N, Schönfeld W, Landek G, Padovan J, Jelić D, Ager A, Milhous WK, Ellis
W, Spaventi R, Ohrt C. Synthesis, structure-activity relationship, and
antimalarial activity of ureas and thioureas of 15-membered azalides. J Med Chem.
2011 May 26;54(10):3595-605. Epub 2011 May 4. PubMed PMID: 21476508.
8. Hutinec A, Rupčić R, Ziher D, Smith KS, Milhous W, Ellis W, Ohrt C, Schönfeld
ZI. An automated, polymer-assisted strategy for the preparation of urea and
thiourea derivatives of 15-membered azalides as potential antimalarial
chemotherapeutics. Bioorg Med Chem. 2011 Mar 1;19(5):1692-701. Epub 2011 Jan 22.
PubMed PMID: 21316974.
9. Kazura JW, Ripp J, Milhous WK. Back to the future: the American Society of Tropical Medicine and Hygiene, its journal and the continuing commitment to global health. Am J Trop Med Hyg. 2011 Jan;84(1):3-4. PubMed PMID: 21212193; PubMed Central PMCID: PMC3005509.
10. Gutteridge CE, Hoffman MM, Bhattacharjee AK, Milhous WK, Gerena L. In vitro efficacy of 7-benzylamino-1-isoquinolinamines against Plasmodium falciparum related to the efficacy of chalcones. Bioorg Med Chem Lett. 2011 Jan 15;21(2):786-9. Epub 2010 Nov 26. PubMed PMID: 21168330.
11. Chattopadhyay R, Velmurugan S, Chakiath C, Andrews Donkor L, Milhous W, Barnwell JW, Collins WE, Hoffman SL. Establishment of an in vitro assay for assessing the effects of drugs on the liver stages of Plasmodium vivax malaria. PLoS One. 2010 Dec 9;5(12):e14275. PubMed PMID: 21151554; PubMed Central PMCID: PMC3000326.
12. Serebrennikova YM, Patel J, Milhous WK, García-Rubio LH. Quantitative analysis of morphological alterations in Plasmodium falciparum infected red blood cells through theoretical interpretation of spectral measurements. J Theor Biol. 2010 Jun 1. [Epub ahead of print] PubMed PMID: 20685319.
13. Whitman TJ, Coyne PE, Magill AJ, Blazes DL, Green MD, Milhous WK, Burgess TH, Freilich D, Tasker SA, Azar RG, Endy TP, Clagett CD, Deye GA, Shanks GD, Martin GJ. An outbreak of Plasmodium falciparum malaria in U.S. Marines deployed to Liberia. Am J Trop Med Hyg. 2010 Aug;83(2):258-65. PubMed PMID: 20682864; PubMed Central PMCID: PMC2911167.
14. Milhous WK and Weina PJ. 2010. Speeding the botanical solution to malaria. Science. 2010 Jan 15;327(5963):279-80.
15. Obaldia N, Milhous W, Kyle D. 2009. Adaptation of a Multidrug Resistant C2A Clone of Plasmodium falciparum from Thailand to Aotus Monkeys and its Preliminary in vivo Antimalarial Drug Resistance Profile. Am J Trop Med Hyg. 2009 Oct;81(4):587-94.PMID: 19815871
16. Happi CT, Gbotosho GO, Folarin OA, Sowunmi A, Hudson T, O'Neil M, Milhous W, Wirth DF, Oduola AM. Selection of Plasmodium falciparum multidrug resistance gene 1 alleles in asexual stages and gametocytes by artemether-lumefantrine in Nigerian children with uncomplicated falciparum malaria. Antimicrob Agents Chemother. 2009 Mar;53(3):888-95. PubMed PMID: 19075074
17. Mui EJ, Schiehser GA, Milhous WK, Hsu H, Roberts CW, Kirisits M, Muench S, Rice D, Dubey JP, Fowble JW, Rathod PK, Queener SF, Liu SR, Jacobus DP, McLeod R. 2009. Target Validation and Efficacy of a Novel Third Generation Antifolate Antimalarial Against Toxoplasmosis. Memorias do Instituto Oswaldo Cruz
18. Šolaja BA, Opsenica D, Smith KS, Milhous WK, Terzić N, Opsenica I, Burnett JC, Nuss J, Gussio R, Bavari S. Novel 4-Aminoquinolines Active against Chloroquine-Resistant and Sensitive P. falciparum Strains that also Inhibit Botulinum Serotype A. J Med Chem. 51:4388-4391 2008 Jul 19. [Epub ahead of print]
19. Opsenica I, Opsenica D, Lanteri CA, Anova L, Milhous WK, Smith KS, Solaja BA. New chimeric antimalarials with 4-aminoquinoline moiety linked to a tetraoxane skeleton. J Med Chem. 2008 Oct 9;51(19):6216-9. Epub 2008 Sep 6. PMID: 18774792 [PubMed - indexed for MEDLINE]
20. Opsenica I, Opsenica D, Smith KS, Milhous WK, Solaja BA. Chemical Stability of the Peroxide Bond Enables Diversified Synthesis of Potent Tetraoxane Antimalarials. J Med Chem. 2008 Apr 10;51(7):2261-6. Epub 2008 Mar 11.
21. Mui EJ, Schiehser GA, Milhous WK, Hsu H, Roberts CW, Kirisits M, Muench S, Rice D, Dubey JP, Fowble JW, Rathod PK, Queener SF, Liu SR, Jacobus DP, McLeod R. Novel Triazine JPC-2067-B Inhibits Toxoplasma gondii In Vitro and In Vivo. PLoS Negl Trop Dis. 2008 Mar 5;2(3):e190. PMID: 18320016
22. Li Q, Weina PJ and Milhous WK. 2007. Pharmacokinetic and pharmacodynamic profiles of rapid-acting astemisinins in antimalarial therapy. Current Drug Therapy 2:210-223
23. Guan J, Wang X, Smith K, Ager A, Gettayacamin M, Kyle DE, Milhous WK, Kozar MP, Magill AJ, Lin AJ. Malaria causal prophylactic activity of imidazolidinedione derivatives.J Med Chem. 2007 Nov 29;50(24):6226-31. Epub 2007 Oct 30. PMID: 17967003
24. Opsenica I, Opsenica D, Jadranin M, Smith K, Milhous WK, Stratakis M and Šolaja B. 2007. On peroxide antimalarials. J Serb Chem Soc 72: 1181–1190.
25. Diaz DS, Kozar MP, Smith KS, Asher CO, Sousa JC, Schiehser GA, Jacobus DP, Milhous WK, Skillman DR, Shearer TW. The role of specific P450 isoforms in the conversion of phenoxypropoxybiguanide analogs in human liver microsomes to potent antimalarial dihydrotriazines. Drug Metab Dispos. 2007 Drug Metab Dispos. 2008 Feb;36(2):380-5. Epub 2007 Nov 15.


Fellows of the Amer Soc Trop Med Hyg
Fellows of the Amer Academy Microbiology
Diplomates of the Amer Board of Med Micro
Antimicrobial Agents & Chemotherapy Editors