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Genetics and Biochemistry Profiles


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Michael Sehorn

Genetics and Biochemistry

Associate Professor

864-656-2572
Life Sciences Building 57A [Office]
Life Sciences Building 60F [Lab]

msehorn@clemson.edu
Website

 

Educational Background

Ph.D., Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center at Shreveport, 2002
B.S., Biology, New Mexico Tech, 1995

Profile/About Me

I am a member of The Center for Optical Materials Science and Engineering Technologies (COMSET) and a member of the Eukaryotic Pathogens Innovation Center (EPIC).

Research Interests

DNA double-strand breaks can result from exposure to endogenous sources such as free radicals and collapsed replication forks or through exposure to exogenous sources such as ionizing radiation. Efficient repair of these breaks is important to maintain genome stability. Homologous recombination (HR) is one of two major pathways that repairs DNA double-strand breaks. Homologous recombination is a major pathway that utilizes a template to mend DNA double-stranded breaks. This pathway contains numerous proteins including the RAD51 and DMC1 recombinases. My laboratory strives to understand the mechanism of homology-directed repair of DNA double-strand breaks mediated by RAD51 and DMC1 in Saccharomyces cerevisiae, Entamoeba histolytica, and humans.

Courses Taught

BCHM 2050: CURES Clinical undergraduate research in emergency and surgery
BCHM 2070: C-MED Shadowing experience for Pre-med
BCHM 3050: Essential Elements of Biochemistry
GEN/BCHM 4910: DNA Repair
GEN/BCHM 4910H: DNA Repair
BCHM 4360/6360/4360H: Molecular Biology: Genes to Proteins
BCHM 4930: Senior Seminar

Selected Publications

Waldvogel, S.M. *, Goodson, S.D., Sehorn, M.G. (2019) Stabilization of the human DMC1 nucleoprotein filament. Methods Mol. Biol. 1999:285-291.

Kelso, A.A. Goodson, S.D., Sehorn, M.G. (2019) In vitro assays for DNA branch migration. Methods Mol. Biol. 1999:271-284.

Goodson, S.D., Hawes, R.B. *, Waldvogel, S.M. *, Sehorn, M.G. (2019) In vitro assay for plasmid length DNA strand exchange by Human DMC1. Methods Mol. Biol. 1999:265-270.

Welter, B.H., Sehorn, M.G., Temesvari, L.A. (2017) Flow cytometric characterization of encystation in Entamoeba invadens. Mol. Biochem. Parasitol. 218:23-27. doi: 10.1016/j.molbiopara.2017.10.002.

Kelso, A.A., Waldvogel, S.M.*, Luthman, A.J.*, Sehorn, M.G. (2017) Homologous recombination in protozoan parasites and recombinase inhibitors. Front. Microbiol. 8:1716. doi: 10.3389/fmicb.2017.01716.

Kelso, A.A., Goodson, S.D., Temesvari, L.A., Sehorn, M.G. (2016) Data on Rad51 amino acid sequences from higher and lower eukaryotic model organisms and parasites. Data Brief. 10:364-368. doi: 10.1016/j.dib.2016.12.002.

Kelso, A.A., Goodson, S.D., Watts, L.E., Ledford, L.L., Waldvogel, S.M., Diehl, J.N., Shah, S.B., Say, A.F., White, J.D., Sehorn, M.G. (2016) The β-isoform of BCCIP promotes ADP release from the RAD51 presynaptic filament and enhances homologous DNA pairing. Nucleic Acids Res. 45(2):711-725. doi: 10.1093/nar/gkw877.

Kelso, A.A., Goodson, S.D., Chavan, S., Say, A.F., Turchick, A., Sharma, D., Ledford, L.L., Ratterman, E., Leskoske, K., King, A.V., Attaway, C.C., Bandera, Y.P., Foulger, S.H., Mazin, A.V., Temesvari, L.A., Sehorn, M.G. (2016) Characterization of the recombination activities of the Entamoeba histolytica Rad51 recombinase. Mol. Biochem. Parasitol. pii: S0166-6851(16)30124-4. doi: 10.1016/j.molbiopara.2016.09.001.

Jenkins, R., Bandera, Y.P., Daniele, M.A., Ledford, L.L., Tietje, A., Kelso, A.A., Sehorn, M.G., Wei, Y., Chakrabarti, M., Ray, S.K., Foulger, S.H. (2016) Sequestering survivin to functionalized nanoparticles: a strategy to enhance apoptosis in cancer cells. Biomater Sci. 4(4):614-26. doi: 10.1039/c5bm00580a.

Kelso, A.A., Say, A.F., Sharma, D., Ledford, L.A., Turchick, A., Saski, C.A., King A.V., Attaway, C.C., Temevari, L.A., Sehorn, M.G. Entamoeba histolytica Dmc1 catalyzes homologous DNA pairing and Strand exchange that is stimulated by Calcium and Hop2-Mnd1. (2015) PLoS One. Â? Sep 30;10(9):e0139399. doi: 10.1371/journal.pone.0139399.

Sharma, D., Say, A.F., Ledford, L.L., Hughes A.J., Sehorn, H.A., Dwyer, D.S., Sehorn, M.G. Role of the conserved lysine within the Walker A Motif of human Dmc1. (2013) DNA Repair. 12(1):53-62.

Daniele, M.A., Bandera, Y.P., Sharma, D., Rungta, P., Roeder, R., Sehorn, M.G., Foulger, S.H. (2012) Substrate-Baited Nanoparticles: A catch and release strategy for enzyme recognition and harvesting. Small. 8(13):2083-90.

Busygina, V., Saro, D., Williams, G., Leung, W.K., Say, A.F., Sehorn, M.G., Sung, P., and Tsubouchi, H. (2011) Novel attributes of Hed1 affect dynamics and activity of Rad51 presynaptic filament during meiotic recombination. J. Biol. Chem. 287(2):1566-75.

Sehorn, M.G., Sehorn, H. A. (2011) Visualization of human Dmc1 presynaptic filaments. Methods Mol. Biol. 745:485-96.

Say, A. F., Ledford, L.A., Sharma, D., Singh, A.K., Leung, W.K., Sehorn, H.A., Tsubouchi, H., Sung, P., Sehorn, M.G. (2011) The budding yeast Mei5-Sae3 complex interacts with Rad51 and preferentially binds a DNA fork structure. DNA Repair. 10(6):586-94.

Rungta, P., Bandera, Y.P., Roeder, R.D., Li, Y., Baldwin, W.S., Sharma, D., Sehorn, M.G., Luzinov, I., Foulger, S.H. (2011) Selective Imaging and Killing of Cancer Cells with Protein Activated Near-Infrared Fluorescing Nanoparticles. Macromol Biosci. 11(7):927-37.

Gaines, W.A., Sehorn, M.G., Marcotte, W.R. Jr. (2010) Spidroin N-terminal domain promotes a pH-dependent association of silk proteins during self-assembly. J. Biol. Chem. 285(52):40745-53.

Chi, P., Kwon, Y., Moses, D. N., Seong, C., Sehorn, M.G., Singh, A.K., Tsubouchi, H., Greene, E.C., Klein, H.L., Sung, P. (2009) Functional Interactions of Meiotic Recombination Factors Rdh54 and Dmc1. DNA Repair 8(2):279-84.

Sheridan, S. D., Yu, X., Roth, R., Heuser, J.E., Sehorn, M.G., Sung, P., Egelman, E.H., Bishop, D.K. (2008) A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments.

Nucleic Acids Res. 36(12):4057-66.

Busygina, V., Sehorn, M.G., Shi, I., Tsubouchi, H., Roeder, S., Sung, P. (2008) Hed1 regulates Rad51-mediated recombination via a novel mechanism. Genes Dev. 22(6):786-95.

Seong, C., Sehorn, M.G., Plate, I., Shi, I., Song, B., Chi, P, Mortensen, U., Sung, P., and Krejci, L. (2008) Molecular anatomy of the recombination mediator function of Saccharomyces cerevisiae Rad52. J. Biol. Chem. 283(18):12166-74.