Lisa J. Bain
- Post-doctoral Associate, Fox Chase Cancer Center, Medical Oncology, 1998
- Ph.D., North Carolina State University, Toxicology, 1997.
- B.S., University of Georgia, Environmental Health Sciences, 1992.
- Understanding the mechanisms by which arsenic causes developmental toxicity
- Determining how ATP-dependent transport proteins alter the metabolism, disposition, and elimination of drugs and toxicants
Research in my laboratory focuses on the mechanisms by which cells respond to toxicants, such as arsenic, chromium, and pharmaceuticals.
The first project investigates how arsenic delays cellular and organismal differentiation and development. Arsenic is a contaminant in drinking water in many parts of the world, and has been found at appreciable levels in rice and juices. Arsenic readily crosses the placental barrier and exposure is correlated with adverse developmental outcomes such as stillbirths, spontaneous abortions, neonatal mortality, low birth weight, delays in the use of musculature, and altered neuronal function. We are examining arsenic’s effects on development and cellular differentiation in both cell lines and fish embryos using microarrays, qPCR, and immunohistochemical techniques.
The second project examines ATP-dependent transport proteins, specifically Mrp1 and Mrp2, which are involved in the elimination of compounds from the liver, kidney, and intestine. This active elimination lowers both the concentration and the toxicity of the compound to the organism. We are examining how these transporters are regulated, what their normal physiological roles are, elucidating toxicants that may interfere with these protein pumps, and whether co-regulation exists between phase I and phase II enzyme systems and transporters.
- Liu JT and Bain LJ (2014) Arsenic inhibits hedgehog signaling during P19 cell differentiation., Toxicol Appl Pharmacol. 2014 Dec 15;281(3):243-53. doi: 10.1016/j.taap.2014.10.007. Epub 2014 Oct 30.
- Hong G-M and Bain LJ (2012) Arsenic exposure inhibits myogenesis and neurogenesis in P19 stem cells through repression of the b-catenin signaling pathway, Toxicological Sciences, 129:146-156.
- Sivils JC, Ancrum TM, and Bain LJ (2012) Loss of Mrp1 alters detoxification enzyme expression in a tissue- and hormonal-status specific manner, Journal of Applied Toxicology, Epub, PMID: 22522787.
- Green BR and Bain LJ (2012) Mrp2 is involved in the efflux and disposition of fosinopril, Journal of Applied Toxicology, Epub, PMID: 22095822.
- Hong G-M and Bain LJ (2012) Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1, Toxicology and Applied Pharmacology, 260:250-259.
- Gaworecki KM, Chapman RW, Neely MG, D’Amico AR, and Bain LJ (2012) Arsenic exposure in killifish during embryogenesis alters muscle development and structure, Toxicological Sciences, 125:522-531.
- Steffens AA, Hong G-M, and Bain LJ (2011) Sodium arsenite delays the differentiation of C2C12 mouse myoblast cells and alters methylation patterns on the transcription factor myogenin, Toxicology and Applied Pharmacology, 250:154-161.
- Gonzalez HO, Hu J, Gaworecki KM, Roling JA, Baldwin WS, Gardea-Torresdey JL, and Bain LJ (2010) Dose-responsive gene expression changes in juvenile and adult mummichogs (Fundulus heteroclitus) after arsenic exposure, Marine Environmental Research, 70:133-141.
- Sivils JC, Gonzalez I, and Bain LJ (2010) Mice lacking MRP1 have reduced testicular steroid hormone levels and alterations in steroid biosynthetic enzymes, General and Comparative Endocrinology, 167:51-59.
- Burnett KG, Bain LJ, Baldwin WS, Callard GV, Cohen S, Di Giulio RT, Evans DH, Comez-Chiarri M, Hahn ME, Hoover CA, Karchner SI, Katoh F, MacLatchy DL, Marshall WS, Meyer JN, Nacci DE, Oleksiak MF, Rees BB, Singer TP, Stegeman JJ, Towle DW, Van Veld PA, Vogelbein WK, Whitehead A, Winn RN, and Crawford DL (2007) Fundulus as the premier teleost model in environmental biology: opportunities for new insights using genomics, Comparative Biochemistry and Physiology part D 2:257-286.
- ENTOX 430/630 Toxicology
- BIOSCI 461/661 Cell Biology
Previous Courses Taught
- Environmental Stressors in the Ecosystem
- General Biology
- Society of Toxicology
- Society of Environmental Toxicology and Chemistry