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Analysis

Molecular characterization and quantification of metabolites and other chemical compounds is a vital technique to all disciplines whose emphasis ranges from genes to ecosystems. Along with the routine quantitation and characterization of compounds that are of natural and synthetic origins, MUAL undertakes method-development for improved and more sensitive quantitation of compounds from biological and environmental matrices through various sample purification/concentration and analytical approaches. 

Some of the routine analysis we have and can perform are listed below along with the data that were collected in our lab.  We closely work with researchers to optimize other procedures for which we have the equipment capacity.  Please contact us to determine feasibility of specific analytical techniques.

Targeted and global metabolomic workflows that we adopt

Flow

 

Data reduction and pathway analysis to contextualize metabolomics data 

Heat MapPathway

 

Extended coverage (red dots) of plant secondary metabolism (eg. flavonoid biosynthesis) using non-targeted metabolomics
 FlavenoidsBiomass
Isobaric Analysis

Isobaric

Following are excerpts of some of the research projects lab we have undertaken in the recent past:
  • Separation and quantification of geometric and positional isomers of fatty acids from milk, meat, and feed samples
  • Stable Isotope Resolved Metabolomic Analysis to trace the amino acid fluxes in plants
  • Analysis of volatile compounds from various biological and environmental matrices
  • Analysis of neurotransmiters from fish brain as a measure of toxicological response
  • Profiling of more than 150 glucosinolates in brassica species
  • Leaching of antibiotics from implants 
  • Quantification of creatine and synthetic analogues from fish brain
  • Amino acid composition of spider silk 
  • Measuement of neonicotenoides in nectar, pollen, honey, and plant tissues
  • Antibiotics in rabbit blood samples
  • Non-targeted metabolomics to characterize the cellular physiology of herbicide resistance in weeds
  • Characterization of plant/microbe biomarkers in soils 
  • Metabolomics of C3 and C4 grasses exposed to various climate treatments
  • Response of plant hormones to various chilling treatments in medicinal plants
  • Runoff potential of pesticides in ornamental nursery operations with respect to pulse irrigation systems
  • Analysis of fatty acids extracted from soils associated with different crop rotation schemes for determination of microbial community associated with soils
  • Analysis of ground beef from various strains of cattle on different feeding regimes for the determination of their effect on saturated and unsaturated fatty acid content
  • Analysis of bacteriocin isolated from Propionibacterium jensenii
  • Analysis of water samples containing various pesticides related to the development of new procedures for extraction of pesticides in water - associated with the Southern Regional Project
  • Analysis of fatty acid utilizing mass spectrometry for the determination of enzyme specificity related to double bond incorporation
  • Identification of microbial isolates associated with the plant problem clinic using gas chromatograph/microbial identification system
  • Analysis of fatty acid from fungi for the development of the usage of Microbial Identification software/system for the identification of species/subspecies of Phytophthora spp
  • Extraction and analysis of fluoranthene and phenanthrene degradation products from bacterial cultures
  • Quantitation/comparison of fatty acids isolated from bacterial cultures grown at various cultural conditions
  • Analysis of berberine from plants grown in tissue culture
  • Identification of various pathogenic isolates of Propionibacterium acnes, P. avidum and P. granulosum utilizing fatty acid profiles which are being tested for the effect of the bactericin produced by Propionibacterium jensenii
  • Analysis of ginkolides from plant tissue associated with tissue cultured plantlets
  • Identification of bacterial aerobes and anaerobes associated with the bacterial wetwood disease complex of oak
  • Identification of microbial degradation products of the fungicide metalaxyl
  • Analysis of gibberellins and its relationship of phytochrome and gibberellins in rice
  • Identification of bacterial isolates associated with wood samples from Chili containing green stain phenomenon
  • Identification of microbial isolates from gravel remediation beds in which isoxaben was applied to determine the change in microbial populations as affected by the herbicide
  • Identification of microbial isolates from cotton bolls affected with lock boll syndrome
  • Assessment of microbial communities associated with solar pasteurization of peach tree short life sites
  • Assessment of microbial communities associated with potting mix which contain a rhizosphere community compared with no rhizosphere after treatment with herbicide