Sachin Rustgi

Assistant Professor of Molecular Breeding and Faculty Scholar in Clemson University School of Health Research
Adjunct Assistant Professor in Dept. of Crop & Soil Sciences at Wash. State Univ., Pullman
Plant and Environmental Sciences Department, PeeDee Research and Education Center

Office: John B. Pitner Center, Pee Dee REC
Phone: 843-519-0475
Email: srustgi@clemson.edu
Vita: Download CV
Personal Website: https://www.clemson.edu/cafls/research/peedee/molecularpltbrd.html

 

 Educational Background

Ph.D. Crop Biotechnology
C.C.S. University, Meerut 2006

M.S. Botany
M.J.P. Rohilkhand University, Bareilly 2001

B.S. Biology and Chemistry
M.J.P. Rohilkhand University, Bareilly 1999

D.I.S.M. Diploma in Information Systems Management
Aptech, Chandausi 2000

 Courses Taught

PES 8900 Crop Biotechnology Journal Club
PLPA 8020 Molecular Plant Pathology Journal Club

 Profile

Dr. Sachin Rustgi is currently affiliated as Assistant Professor in Molecular Breeding with the Department of Plant and Environmental Sciences at Clemson University, and Adjunct Assistant Professor with the Department of Crop and Soil Sciences at the Washington State University, Pullman. He holds a master’s degree in Botany from MJP Rohilkhand University, Bareilly and a Ph.D. degree in Crop Biotechnology from CCS University, Meerut. Dr. Rustgi’s research largely focuses on the improvement of nutritional value in the small grain cereals and legumes especially by elimination or detoxification of antinutritional elements and enhancement of health-promoting factors. In order to achieve these objectives, he has developed a multidisciplinary team of experts across different Colleges of the Clemson University, the Medical University of South Carolina and the Greenville Health System. His research has resulted in a number of high impact publications in internationally recognized journals including the Proceedings of the National Academy of Sciences of the United States of America, the Journal of Experimental Botany, Plant Molecular Biology, Heredity, PLoS One, and Nutrients, to name a few. In addition to it, Dr. Rustgi authored two monographic books on his research. In view of his academic contributions, he was awarded with the 2014 Faculty Excellence in Research Award of the Washington State University College of Agricultural, Human, and Natural Resource Sciences and invited to join as a member of the Wheat Initiative Expert Working Group on Wheat Quality for Processing and Health and the American Society for Nutrition.

 Research Interests

My current research focus is bipartite: On one hand, I am involved in the understanding of basic plant processes such as transport of proteins and transfer RNA from the cytoplasm to the endosymbiotic organelles (mitochondria and chloroplast) in the plant cells, and the molecular mechanism of insect pest deterrence in plants. And on the other hand, I focus on the development of novel dietary therapies for the ‘gluten syndrome’ and other two major food allergens, peanuts, and soybean, as well as the development of novel RNA interference based pesticides for major insect pests in the Southeast US.

 Publications

Total publications 76; Citations 3004; h-index 22; i10-index 31

[A] Refereed publications
1. Rustgi, S., Shewry, P., Brouns, F., Deleu, L., And Delcour, J.A., “Wheat seed proteins - factors influencing their content, composition, and technological properties, and strategies to reduce adverse reactions,” Comprehensive Reviews in Food Science and Food, (2019), in press.
2. Brouns, F., Van Rooy, G., Shewry, P., Rustgi, S., and Jonkers, D., “Adverse reactions to wheat or wheat components,” Comprehensive Reviews in Food Science and Food Safety, (2019). In press
3. Rustgi, S., Shewry, P., and Brouns, F., “Health hazards associated with gluten consumption in susceptible individuals and current status of research on the dietary therapies,” In: Igrejas, G. (ed.), ‘Wheat Quality for Improving Processing and Human Health’, Springer, (2019). In press
4. Cong, X., Miao, Y., Xu, L., Zhang, Y., Yuan, C., Wang, J., Zhuang, T., Lin, X., Jiang, L., Wang, N., Ma, J., Sanguinet, K.A., Liu, B., Rustgi, S., and Ou, X., “Transgenerational memory of gene expression changes induced by heavy metal stress in rice (Oryza sativa L.),” BMC Plant Biology (2019). In press
5. Yang, M., Liu, Y., Dong, J., Zhao, W., Kashyap, S., Gao, X., Rustgi, S., and Wen, S., “Probing early wheat grain development via transcriptomic and proteomic approaches,” Functional & Integrative Genomics, (2019). In press
6. Rustgi, S., Springer, A., Kang, C., von Wettstein, D., Reinbothe, C., Reinbothe S., and Pollmann, S, “Two non-canonical cytochrome P450s in Arabidopsis thaliana and their different roles in plant defense,” International Journal of Molecular Sciences, 20, 3064 (2019).
7. Pollmann, S., Springer, A., Rustgi, S., von Wettstein, D., Kang, C., Reinbothe, C., and Reinbothe, S., “Substrate channeling in oxylipin biosynthesis through a protein complex in the plastid envelope of Arabidopsis thaliana,” Journal of Experimental Botany, 70, 1483-1495 (2019).
8. Osorio, C.E., Wen, N., Mejias, J.H., Liu, B., Reinbothe, S., von Wettstein, D., and Rustgi, S., “Development of wheat genotypes expressing a glutamine-specific endoprotease from barley and a prolyl endopeptidase from Flavobacterium meningosepticum or Pyrococcus furiosus as a potential remedy to celiac disease,” Functional & Integrative Genomics, 19, 123-136 (2019).
9. Pollmann, S., Springer, A., Rustgi, S., von Wettstein, D., Kang, C., Reinbothe, C., and Reinbothe, S., “Substrate channeling in oxylipin biosynthesis through a protein complex in the plastid envelope of Arabidopsis thaliana,” bioRxiv, doi: https://doi.org/10.1101/286864 (2018).
10. Gill, U.S., Sun, L., Rustgi, S., Tang, Y., von Wettstein, D., and Mysore, K.S., “Transcriptome-based analyses of phosphite-mediated suppression of rust pathogens Puccinia emaculata and Phakopsora pachyrhizi and functional characterization of selected fungal target genes,” The Plant Journal, 93, 894-904 (2018).
11. Rustgi, S., Boex-Fontvieille, E., Reinbothe, C., von Wettstein, D., and Reinbothe, S., “The complex world of plant protease inhibitors: Insights into a Kunitz-type cysteine protease inhibitor of Arabidopsis thaliana,” Communicative & Integrative Biology, 11, 1, e1368599 (2018).
12. Rossig, C., Gray, J., Valdes, O., Rustgi, S., von Wettstein, D., Reinbothe, C., and Reinbothe, S., “HP30-2, a mitochondrial PRAT protein for import of signal sequence-less precursor proteins in Arabidopsis thaliana,” Journal of Integrative Plant Biology, 59, 535-551 (2017).
13. Yang, M.M., Gao, X., Dong, J., Gandhi, N., Cai, H., von Wettstein, D., Rustgi, S., and Wen, S., “Pattern of protein expression in developing wheat grains identified through proteomic analysis,” Frontiers in Plant Science, 8, 962 (2017).
14. Buhr, F., Lahroussi, A., Springer, A., Rustgi, S., von Wettstein, D., Reinbothe, C., and Reinbothe, S., “NADPH:protochlorophyllide oxidoreductase B (PORB) action in Arabidopsis thaliana revisited through transgenic expression of engineered barley PORB mutant proteins,” Plant Molecular Biology, 94, 45-59 (2017).
15. Rustgi, S., Boex-Fontvieille, E., Reinbothe, C., von Wettstein, D., and Reinbothe, S., “Serpin 1 and the water-soluble chlorophyll binding Kunitz protease inhibitor WSCP differentially regulate the activity of the cysteine protease RD21 (RESPONSIVE TO DESICCATION) during plant development in Arabidopsis thaliana,” Proceedings of the National Academy of Sciences, the United States of America, 114, 2212–2217 (2017).
16. Rustgi, S., Ankrah, N., Brew-Appiah, R.A.T., Sun, Y., Liu, W., and von Wettstein, D., “Doubled haploid transgenic wheat lines by microspore transformation,” Methods in Molecular Biology, 1679, 213-234 (2017).
17. Boex-Fontvieille, E., Rustgi, S., von Wettstein, D., Pollmann, S., Reinbothe, S., and Reinbothe, C., “An ethylene-protected Achilles’ heel of etiolated seedlings for arthropod deterrence,” Frontiers in Plant Science 7, 1246 (2016).
18. Boex-Fontvieille, E., Rustgi, S., von Wettstein, D., Pollmann, S., Reinbothe, S., and Reinbothe, C., “Jasmonic acid protects etiolated seedlings of Arabidopsis thaliana against herbivorous arthropods,” Plant Signaling and Behavior 11, e1214349 (2016).
19. Springer, A., Kang, C., Rustgi, S., von Wettstein, D., Reinbothe, C., Pollmann, S., and Reinbothe, S., “Programmed chloroplast destruction during leaf senescence involves 13-lipoxygenase (13-LOX),” Proceedings of the National Academy of Sciences, the United States of America, 113, 3383–3388 (2016).
20. Gray, J., Rustgi, S., von Wettstein, D., Reinbothe, C., and Reinbothe, S., “Common functions of the chloroplast and mitochondrial co-chaperones cpDnaJL (CDF1) and mtDnaJ (PAM16) in protein import and ROS scavenging in Arabidopsis thaliana,” Communicative & Integrative Biology, 9, e1119343 (2015).
21. Boex-Fontvieille, E., Rustgi, S., Reinbothe, S., and Reinbothe, C., “A Kunitz-type protease inhibitor regulates programmed cell death during flower development in Arabidopsis thaliana,” Journal of Experimental Botany, 66, 6119-6135 (2015).
22. Boex-Fontvieille, E., Rustgi, S., von Wettstein, D., Reinbothe, S., and Reinbothe, C., “Water-Soluble chlorophyll protein (WSCP) is involved in light-dependent herbivore resistance activation during greening of Arabidopsis thaliana,” Proceedings of the National Academy of Sciences, the United States of America, 112, 7303–7308 (2015).
23. Rustgi, S., and von Wettetsin, D., “Breeding barley ornamented with the novel agronomical attributes,” Medicinal & Aromatic Plants, 4, e158 (2015).
24. Reinbothe, S., Gray, J., Rustgi, S., von Wettstein, D., and Reinbothe, C., “Cell growth defect factor 1 is crucial for the plastid import of NADPH:protochlorophyllide oxidoreductase A in Arabidopsis thaliana,” Proceedings of the National Academy of Sciences, the United States of America, 112, 838–5843 (2015).
25. Ou, X., Zhuang, T., Yin, W., Miao, Y., Zhang, Y., Lin, X., Xu, C., von Wettstein, D., Rustgi, S., and Liu, B., “DNA methylation changes induced in rice by exposure to high concentrations of the nitric oxide modulator, sodium nitroprusside,” Plant Molecular Biology Reporter, 33, 1428-1440 (2015).
26. Rustgi, S., Pollmann, S., Buhr, F,. Springer, A,. Reinbothe, C., von Wettstein, D., and Reinbothe. S., “JIP60-mediated, jasmonate- and senescence-induced molecular switch in translation toward stress and defense protein synthesis,” Proceedings of the National Academy of Sciences, the United States of America, 111:14181-14186 (2014).
27. Bhullar, R., Nagarajan, R., Bennypaul, H., Sidhu, G.K., Sidhu, G., Rustgi, S., von Wettstein, D., and Gill, K.S., “Silencing of a metaphase I-specific gene results in a phenotype similar to that of the Pairing homeologous 1 (Ph1) gene mutations,” Proceedings of the National Academy of Sciences, the United States of America, 111, 14187-14192 (2014).
28. Yang, C., Zhao, L., Yang, Z., Xu, C., Zhang, H., Wen, S., Zhang, C., Rustgi, S., von Wettstein, D., and Liu, B., “Evolution of physiological responses to salt stress in hexaploid wheat,” Proceedings of the National Academy of Sciences, the United States of America, 111, 11882-11887 (2014).
29. Hu, L., Li, N., Xu, C., Zhong, S., Lin, X., Yang, J., Zhou, T., Yuliang, A., Cao, X., Zemach, A., Rustgi, S., von Wettstein, D., and Liu, B., “Mutation of a major CG methylase in rice causes genome-wide hypomethylation, dysregulated genome expression, and seedling lethality,” Proceedings of the National Academy of Sciences, the United States of America, 111, 10642-10647 (2014).
30. Rustgi, S., Matanguihan, J., Mejías, J., Gemini, R., Osorio, C., Wen, N., Ankrah, N., Brew-Appiah, R., Murphy, K.M., and von Wettstein, D., “Assessment of Genetic Diversity among Barley Cultivars and Breeding Lines Adapted to the US Pacific Northwest, and Its Implications in Breeding Barley for Imidazolinone-Resistance,” PLOS One, 9, e100998 (2014).
31. Mejias, J.H., Lu, X., Osorio, C., Ullman, J.L., von Wettstein, D., and Rustgi, S., “Analysis of wheat prolamins, the causative agents of celiac sprue, using reversed phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS),” Nutrients, 6, 1578–1597 (2014).
32. Zhang, H., Bian, Y., Gou, X., Zhu, B., Xu, C., Qi, B., Li, N., Rustgi, S., Zhou, H., Han, F., Jiang, J., von Wettstein, D., and Liu, B., “Persistent whole-chromosome aneuploidy is generally associated with nascent allohexaploid wheat,” Proceedings of the National Academy of Sciences, the United States of America, 110, 3447-3452 (2013).
33. Gupta, P.K., Rustgi, S., and Mir, R.R., “Array-based high-throughput DNA markers for cereal genetics and genomics,” In: Gupta, P.K., and Varshney, R.K., editors, Cereal Genomics II, Springer, Dordrecht, pp 11-55 (2013).
34. Kumar, N., and Rustgi, S., “Agronomically relevant traits transferred to major crop plants by alien introgressions,” In: Pratap, A., and Kumar, J., editors, Alien Gene Transfer in Crop Plants, Volume 1, Springer, New York, pp 211-245.
35. Rustgi, S., Shafqat, M.N., Kumar, N., Ali, M.L., Dweikat, I., Baenziger, P., and Gill, K.S., “Genetic dissection of yield and its component traits using high-density composite map of wheat chromosome 3A: Bridging gaps between QTLs and underlying genes,” PLoS ONE 8, e70526 (2013).
36. Rustgi, S., “Engineering wheat genotypes compatible for gluten sensitive, allergenic and intolerant individuals,” International Journal of Plant Biology & Research, 1, 1003 (2013).
37. Brew-Appiah, R.A.T., Ankrah, N., Liu, W., Konzak, C.F., von Wettstein, D., and Rustgi, S., “Generation of doubled haploid transgenic wheat lines by microspore transformation,” PLoS ONE, 8, e80155 (2013).
38. Zhang, H., Bian, Y., Gou, X., Dong, Y., Rustgi, S., Zhang, B., Xu, C., Li, N., Qi, B., Han, F., von Wettstein, D., and Liu, B., “Intrinsic karyotype stability and gene copy number variations may have laid the foundation for tetraploid wheat formation,” Proceedings of the National Academy of Sciences, the United States of America, 110, 19466-19471 (2013).
39. Rustgi, S., “Bringing barley back in crop rotation by breeding for imidazolinone resistance,” Medicinal & Aromatic Plants, 2, e148 (2013).
40. Bennypaul, H.S., Mutti, J.S., Rustgi, S., Kumar, N., Okubara, P.A., and Gill, K.S., “Virus-induced gene silencing (VIGS) of genes expressed in root, leaf and meiotic tissues of wheat,” Functional & Integrative Genomics, 12, 143-156 (2012).
41. Osorio, C., Wen, N., Gemini, R., Zemetra, R., von Wettstein, D., and Rustgi, S. “Targeted modification of wheat grain protein to reduce the content of celiac causing epitopes,” Functional & Integrative Genomics, 12, 417-438 (2012).
42. Wen, S., Wen, N., Pang, J., Langen, G., Brew-Appiah, R.A.T., Mejias, J.H., Osorio, C., Yang, M.M., Gemini, R., Moehs, C.P., Zemetra, R.S., Kogel, K.H., Liu, B., Wang, X., von Wettstein, D., and Rustgi, S., “Structural genes of wheat and barley 5-methylcytosine DNA glycosylases and their potential applications for human health,” Proceedings of the National Academy of Sciences, the United States of America, 109, 20543-20548 (2012).
43. Riar, D.S., Rustgi, S., Burke, I.C., Gill, K.S., and Yenish, J.P., “EST-SSR development from 5 Lactuca species and their use in studying genetic diversity among L. serriola biotypes,” Journal of Heredity, 102, 17-28 (2011).
44. Lee, H., Rustgi, S., Kumar, N., Burke, I.C., Yenish, J.P., Gill, K.S., von Wettstein, D., and Ullrich, S.E., “Single nucleotide mutation in the barley acetohydroxy acid synthase (AHAS) gene confers resistance to imidazolinone herbicides,” Proceedings of the National Academy of Sciences, the United States of America, 108, 8909-8913 (2011).
45. Bandopadhyay, R., Rustgi, S., Chaudhuri, R.K., Khurana, P., Khurana, J.P., Tyagi, A.K., Balyan, H.S., Houben, A., and Gupta, P.K., “Use of methylation filtration and C0t fractionation for analysis of genome composition and comparative genomics in bread wheat,” Journal of Genetics & Genomics, 38, 315-325 (2011).
46. Rustgi, S., Balyan, H.S., and Gupta, P.K., “MSL and SNPL: Softwares for in silico mining of SSRs and SNPs,” International Journal of Biotechnology & Biosciences, 1, 353-355 (2011).
47. Baenziger, P.S., Dweikat, I., Gill, K., Eskridge, K., Berke, T., Shah, M., Campbell, B.T., Ali, M.L., Mengistu, N., Mahmood, A., Auvuchanon, A., Yen, Y., Rustgi, S., Moreno-Sevilla, B., Mujeeb-Kazi, A., and Morris, R.M., “Understanding grain yield: It is a journey, not a destination,” Czech Journal of Genetics and Plant Breeding, 47, S77-S84 (2011).
48. Rustgi, S., Bandopadhyay, R., Balyan, H.S., and Gupta, P.K., “EST-SNPs in bread wheat: Discovery, validation, genotyping and haplotype structure,” Czech Journal of Genetics and Plant Breeding, 45, 106-116 (2009).
49. Sidhu, G.K., Rustgi, S., Shafqat, M.N., von Wettstein, D., and Gill, K.S., “Fine structure mapping of a gene-rich region of wheat carrying Ph1, a suppressor of crossing over between homoeologous chromosomes,” Proceedings of the National Academy of Sciences, the United States of America, 105, 5815-5820 (2008).
50. Gupta, P.K., Rustgi, S., and Mir, R.R., “Array-based high-throughput DNA markers for crop improvement,” Heredity, 101, 5-18 (2008).
51. Mir, R.R., Rustgi, S., Sharma, S., Singh, R., Goyal, A., Kumar, J., Gaur, A., Tyagi, A.K., Khan, H., Sinha, M.K., Balyan, H.S., and Gupta, P.K., “A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute,” Euphytica, 161, 413-427 (2008).

[B] Books and Monographs
1. Rustgi, S., In silico development and use of SSR and SNP markers in bread wheat: Gene derived markers and genome fractionation a glimpse in the wheat genome, 1st edition (2011), VDM Verlag Dr. Mueller GmbH & Co. KG, Saarbrücken, Germany.
2. Rustgi, S., Wen, N., Osorio, C., Brew-Appiah, R.A.T., Wen, S., Gemini, R., Mejias, J.H., Ankrah, N., Moehs, C.P., and von Wettstein, D., Natural dietary therapies for the ‘gluten syndrome’, 1st edition (2014), The Royal Danish Academy of Sciences and Letters, Copenhagen, Denmark.

[C] Other Scholarly Publications
1. Rustgi, S., Kashyap, S., Gandhi, N., von Wettstein, D., Ankrah, N., Gemini, R., and Reisenauer, P., “Novel wheat genotypes designed to meet the future needs for safe and surplus food,” Annual Wheat Newsletter 64, 56-61 (2018).
2. Rustgi, S., Gandhi, N., von Wettstein, D., Ankrah, N., Gemini, R., and Reisenauer, P., “Celiac-safe wheat genotypes: A target not beyond reach,” Annual Wheat Newsletter 63, 85-88 (2017).
3. Rustgi, S., von Wettstein, D., Ankrah, N., Ou, X., Sun, Y., and Gemini, R., “Quest for celiac-safe wheat,” Annual Wheat Newsletter, 62, 89-92 (2016).
4. Balyan, H.S., Varshney, R.K., Roy, J.K., Prasad, M., Kulwal, P.L., Kumar, N., Rustgi, S., Kumar, A., Mir, R.R., and Jaiswal, V., “From development of molecular markers to marker-assisted breeding: A successful journey,” The International Conference on Genomics and Translational Research in Crop Improvement, C.C.S. University, Meerut (December 2016).
5. Rustgi, S., von Wettstein, D., Ankrah, N., Brew-Appiah, R.A.T., Wen, N., Mitchell, S.M., Gemini, R., and Reisenauer, P., “Celiac-safe wheat genotypes: A dietary solution to the gluten-induced disorders,” Annual Wheat Newsletter, 61, 99-103 (2015).
6. Rustgi, S., von Wettstein, D., Ankrah, N., Brew-Appiah, R.A.T., Wen, N., Mitchell, S.M., Gemini, R., Reisenauer, P., and Brabb, I., “Breeding celiac-safe wheat cultivars a future market class of wheat,” Annual Wheat Newsletter, 60, 143-146 (2014).
7. Rustgi, S., “Discussion with Assistant Research Professor Sachin Rustgi on the genetic modification of wheat to make it safe for celiacs,” Journal of Gluten Sensitivity, 13,11-14 (2014).
8. Rustgi, S., von Wettstein, D., Ankrah, N., Mejias, J.H., Brew-Appiah, R.A.T., Wen, S., Wen, N., Osorio, C., Gemini, R., Reisenauer, P., Mohan, J., and Brabb, I., “A natural dietary-therapy for gluten intolerance, sensitivity and allergenicity,” Annual Wheat Newsletter, 59, 151-155 (2013).
9. Rustgi, S., von Wettstein, D., Ankrah, N., Brew-Appiah, R.A.T., Wen, S., Wen, N., Osorio, C., Gemini, R., Reisenauer, P., Lu, X., and Mejias, J.H., “Engineering wheat for celiac patients,” Annual Wheat Newsletter, 58, 248-253 (2012).
10. von Wettstein, D., Rustgi, S., Kannangara, C.G., Ankrah, N., Brew-Appiah, R.A.T., Wen, S., Wen, N., Gemini, R., Brueggeman, R., Reisenauer, P., Gill, K.S., Moehs, C.P., Liu, B., Pang, J., Wang, X., Langen, G., and Kogel, K.H., “Multipronged approach to develop nutritionally improved celiac safe wheat varieties,” Annual Wheat Newsletter, 56, 261-264 (2010).

 Links

Faculty Scholar CU School of Health Research
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Member CU Advanced Plant Technology Program